The bacterial biocontrol agent Paenibacillus alvei K165 confers inherited resistance to Verticillium dahliae

The biocontrol agent Paenibacillus alvei K165 was previously shown to protect Arabidopsis thaliana plants against Verticillium dahliae. Here we show that K165 also confers inherited immune resistance to V. dahliae. By performing a histone acetyltransferases mutant screen, ChIP assays, and transcript...

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Autores principales: Gkizi, Danai, González Gil, Anna, Pardal, Alonso J, Piquerez, Sophie J M, Sergaki, Chrysi, Ntoukakis, Vardis, Tjamos, Sotirios E
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Research Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163062/
https://www.ncbi.nlm.nih.gov/pubmed/33829257
http://dx.doi.org/10.1093/jxb/erab154
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author Gkizi, Danai
González Gil, Anna
Pardal, Alonso J
Piquerez, Sophie J M
Sergaki, Chrysi
Ntoukakis, Vardis
Tjamos, Sotirios E
author_facet Gkizi, Danai
González Gil, Anna
Pardal, Alonso J
Piquerez, Sophie J M
Sergaki, Chrysi
Ntoukakis, Vardis
Tjamos, Sotirios E
author_sort Gkizi, Danai
collection PubMed
description The biocontrol agent Paenibacillus alvei K165 was previously shown to protect Arabidopsis thaliana plants against Verticillium dahliae. Here we show that K165 also confers inherited immune resistance to V. dahliae. By performing a histone acetyltransferases mutant screen, ChIP assays, and transcriptomic experiments, we were able to show that histone acetylation significantly contributes to the K165 biocontrol activity and establishment of inheritable resistance to V. dahliae. K165 treatment primed the expression of immune-related marker genes and the cinnamyl alcohol dehydrogenase gene CAD3 through the function of histone acetyltransferases. Our results reveal that offspring of plants treated with K165 have primed immunity and enhanced lignification, both contributing towards the K165-mediated inherited immune resistance. Thus, our study paves the way for the use of biocontrol agents for the establishment of inheritable resistance to agronomically important pathogens.
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spelling pubmed-81630622021-06-02 The bacterial biocontrol agent Paenibacillus alvei K165 confers inherited resistance to Verticillium dahliae Gkizi, Danai González Gil, Anna Pardal, Alonso J Piquerez, Sophie J M Sergaki, Chrysi Ntoukakis, Vardis Tjamos, Sotirios E J Exp Bot Research Papers The biocontrol agent Paenibacillus alvei K165 was previously shown to protect Arabidopsis thaliana plants against Verticillium dahliae. Here we show that K165 also confers inherited immune resistance to V. dahliae. By performing a histone acetyltransferases mutant screen, ChIP assays, and transcriptomic experiments, we were able to show that histone acetylation significantly contributes to the K165 biocontrol activity and establishment of inheritable resistance to V. dahliae. K165 treatment primed the expression of immune-related marker genes and the cinnamyl alcohol dehydrogenase gene CAD3 through the function of histone acetyltransferases. Our results reveal that offspring of plants treated with K165 have primed immunity and enhanced lignification, both contributing towards the K165-mediated inherited immune resistance. Thus, our study paves the way for the use of biocontrol agents for the establishment of inheritable resistance to agronomically important pathogens. Oxford University Press 2021-04-08 /pmc/articles/PMC8163062/ /pubmed/33829257 http://dx.doi.org/10.1093/jxb/erab154 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biology. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Papers
Gkizi, Danai
González Gil, Anna
Pardal, Alonso J
Piquerez, Sophie J M
Sergaki, Chrysi
Ntoukakis, Vardis
Tjamos, Sotirios E
The bacterial biocontrol agent Paenibacillus alvei K165 confers inherited resistance to Verticillium dahliae
title The bacterial biocontrol agent Paenibacillus alvei K165 confers inherited resistance to Verticillium dahliae
title_full The bacterial biocontrol agent Paenibacillus alvei K165 confers inherited resistance to Verticillium dahliae
title_fullStr The bacterial biocontrol agent Paenibacillus alvei K165 confers inherited resistance to Verticillium dahliae
title_full_unstemmed The bacterial biocontrol agent Paenibacillus alvei K165 confers inherited resistance to Verticillium dahliae
title_short The bacterial biocontrol agent Paenibacillus alvei K165 confers inherited resistance to Verticillium dahliae
title_sort bacterial biocontrol agent paenibacillus alvei k165 confers inherited resistance to verticillium dahliae
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163062/
https://www.ncbi.nlm.nih.gov/pubmed/33829257
http://dx.doi.org/10.1093/jxb/erab154
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