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Molecular Epidemiology of Human Papillomaviruses, Neisseria gonorrhoeae, Chlamydia trachomatis and Mycoplasma genitalium among Female Sex Workers in Burkina Faso: Prevalence, Coinfections and Drug Resistance Genes

Viral and bacterial infections represent an occupational risk for female sex workers. This study aimed at determining HPV coinfection with genital pathogens among female sex workers in West and Central Africa and identifying antibiotic resistance genes. A total of 182 samples from female sex workers...

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Autores principales: Tovo, Sessi Frida, Zohoncon, Théodora Mahoukèdè, Dabiré, Amana Metuor, Ilboudo, Régine, Tiemtoré, Rahimatou Yasmine, Obiri-Yeboah, Dorcas, Yonli, Albert Théophane, Dovo, Essi Etonam, Ouédraogo, Rogomenoma Alice, Ouattara, Abdoul Karim, Sorgho, Pegdwende Abel, Ouermi, Djénéba, Djigma, Florencia Wendkuuni, Ouédraogo, Charlemagne, Sangaré, Lassana, Simpore, Jacques
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163177/
https://www.ncbi.nlm.nih.gov/pubmed/34072200
http://dx.doi.org/10.3390/tropicalmed6020090
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author Tovo, Sessi Frida
Zohoncon, Théodora Mahoukèdè
Dabiré, Amana Metuor
Ilboudo, Régine
Tiemtoré, Rahimatou Yasmine
Obiri-Yeboah, Dorcas
Yonli, Albert Théophane
Dovo, Essi Etonam
Ouédraogo, Rogomenoma Alice
Ouattara, Abdoul Karim
Sorgho, Pegdwende Abel
Ouermi, Djénéba
Djigma, Florencia Wendkuuni
Ouédraogo, Charlemagne
Sangaré, Lassana
Simpore, Jacques
author_facet Tovo, Sessi Frida
Zohoncon, Théodora Mahoukèdè
Dabiré, Amana Metuor
Ilboudo, Régine
Tiemtoré, Rahimatou Yasmine
Obiri-Yeboah, Dorcas
Yonli, Albert Théophane
Dovo, Essi Etonam
Ouédraogo, Rogomenoma Alice
Ouattara, Abdoul Karim
Sorgho, Pegdwende Abel
Ouermi, Djénéba
Djigma, Florencia Wendkuuni
Ouédraogo, Charlemagne
Sangaré, Lassana
Simpore, Jacques
author_sort Tovo, Sessi Frida
collection PubMed
description Viral and bacterial infections represent an occupational risk for female sex workers. This study aimed at determining HPV coinfection with genital pathogens among female sex workers in West and Central Africa and identifying antibiotic resistance genes. A total of 182 samples from female sex workers were analyzed by real-time PCR and classic PCR. For the molecular diagnosis of HPV, the real-time multiplex amplification kit “HPV Genotypes 14 Real-TM Quant” from SACACE Biotechnologies(®), detecting 14 high-risk HPV genotypes, was used, while for other pathogens, the real-time multiplex amplification kit N. gonorrhoeae/C. trachomatis/M. genitalium/T. vaginalis Real-TM, allowing their simultaneous detection, was used. The women were aged 17–50 years with an average age of 27.12 ± 6.09 years. The pathogens identified were HPV 54.94% (100/120), Neisseria gonorrhoeae (13.74%), Chlamydia trachomatis (11.54%) and Mycoplasma genitalium (11.54%). The most common HPV genotypes were HPV68, HPV38 and HPV52. The antibiotic resistance genes identified were bla (QNR B) 24.00%, bla (GES) 22.00%, bla (SHV) 17.00%, bla (CTX-M) 13.00% and bla (QNR S) 1.00%. This study revealed the presence of various HPV genotypes associated with other pathogens with problems of antibiotic resistance among sex workers of West and Central African origin working in Ouagadougou.
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spelling pubmed-81631772021-05-29 Molecular Epidemiology of Human Papillomaviruses, Neisseria gonorrhoeae, Chlamydia trachomatis and Mycoplasma genitalium among Female Sex Workers in Burkina Faso: Prevalence, Coinfections and Drug Resistance Genes Tovo, Sessi Frida Zohoncon, Théodora Mahoukèdè Dabiré, Amana Metuor Ilboudo, Régine Tiemtoré, Rahimatou Yasmine Obiri-Yeboah, Dorcas Yonli, Albert Théophane Dovo, Essi Etonam Ouédraogo, Rogomenoma Alice Ouattara, Abdoul Karim Sorgho, Pegdwende Abel Ouermi, Djénéba Djigma, Florencia Wendkuuni Ouédraogo, Charlemagne Sangaré, Lassana Simpore, Jacques Trop Med Infect Dis Article Viral and bacterial infections represent an occupational risk for female sex workers. This study aimed at determining HPV coinfection with genital pathogens among female sex workers in West and Central Africa and identifying antibiotic resistance genes. A total of 182 samples from female sex workers were analyzed by real-time PCR and classic PCR. For the molecular diagnosis of HPV, the real-time multiplex amplification kit “HPV Genotypes 14 Real-TM Quant” from SACACE Biotechnologies(®), detecting 14 high-risk HPV genotypes, was used, while for other pathogens, the real-time multiplex amplification kit N. gonorrhoeae/C. trachomatis/M. genitalium/T. vaginalis Real-TM, allowing their simultaneous detection, was used. The women were aged 17–50 years with an average age of 27.12 ± 6.09 years. The pathogens identified were HPV 54.94% (100/120), Neisseria gonorrhoeae (13.74%), Chlamydia trachomatis (11.54%) and Mycoplasma genitalium (11.54%). The most common HPV genotypes were HPV68, HPV38 and HPV52. The antibiotic resistance genes identified were bla (QNR B) 24.00%, bla (GES) 22.00%, bla (SHV) 17.00%, bla (CTX-M) 13.00% and bla (QNR S) 1.00%. This study revealed the presence of various HPV genotypes associated with other pathogens with problems of antibiotic resistance among sex workers of West and Central African origin working in Ouagadougou. MDPI 2021-05-27 /pmc/articles/PMC8163177/ /pubmed/34072200 http://dx.doi.org/10.3390/tropicalmed6020090 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tovo, Sessi Frida
Zohoncon, Théodora Mahoukèdè
Dabiré, Amana Metuor
Ilboudo, Régine
Tiemtoré, Rahimatou Yasmine
Obiri-Yeboah, Dorcas
Yonli, Albert Théophane
Dovo, Essi Etonam
Ouédraogo, Rogomenoma Alice
Ouattara, Abdoul Karim
Sorgho, Pegdwende Abel
Ouermi, Djénéba
Djigma, Florencia Wendkuuni
Ouédraogo, Charlemagne
Sangaré, Lassana
Simpore, Jacques
Molecular Epidemiology of Human Papillomaviruses, Neisseria gonorrhoeae, Chlamydia trachomatis and Mycoplasma genitalium among Female Sex Workers in Burkina Faso: Prevalence, Coinfections and Drug Resistance Genes
title Molecular Epidemiology of Human Papillomaviruses, Neisseria gonorrhoeae, Chlamydia trachomatis and Mycoplasma genitalium among Female Sex Workers in Burkina Faso: Prevalence, Coinfections and Drug Resistance Genes
title_full Molecular Epidemiology of Human Papillomaviruses, Neisseria gonorrhoeae, Chlamydia trachomatis and Mycoplasma genitalium among Female Sex Workers in Burkina Faso: Prevalence, Coinfections and Drug Resistance Genes
title_fullStr Molecular Epidemiology of Human Papillomaviruses, Neisseria gonorrhoeae, Chlamydia trachomatis and Mycoplasma genitalium among Female Sex Workers in Burkina Faso: Prevalence, Coinfections and Drug Resistance Genes
title_full_unstemmed Molecular Epidemiology of Human Papillomaviruses, Neisseria gonorrhoeae, Chlamydia trachomatis and Mycoplasma genitalium among Female Sex Workers in Burkina Faso: Prevalence, Coinfections and Drug Resistance Genes
title_short Molecular Epidemiology of Human Papillomaviruses, Neisseria gonorrhoeae, Chlamydia trachomatis and Mycoplasma genitalium among Female Sex Workers in Burkina Faso: Prevalence, Coinfections and Drug Resistance Genes
title_sort molecular epidemiology of human papillomaviruses, neisseria gonorrhoeae, chlamydia trachomatis and mycoplasma genitalium among female sex workers in burkina faso: prevalence, coinfections and drug resistance genes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163177/
https://www.ncbi.nlm.nih.gov/pubmed/34072200
http://dx.doi.org/10.3390/tropicalmed6020090
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