A single amino acid Gly-tag enables metal-free protein purification

Analytically pure proteins are indispensable for diverse applications, including therapeutics. Here, we report a methodology where a single amino acid, glycine, enables metal-free protein purification. This robust platform is enabled by a Gly-tag resin for site-specific capture, enrichment, and rele...

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Detalles Bibliográficos
Autores principales: Purushottam, Landa, V. B., Unnikrishnan, Rawale, Dattatraya Gautam, Gujrati, Mansi, Mishra, Surya Dev, T. K., Sajeev, Reddy, Neelesh C., Adusumalli, Srinivasa Rao, Mishra, Ram Kumar, Rai, Vishal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163197/
https://www.ncbi.nlm.nih.gov/pubmed/34094495
http://dx.doi.org/10.1039/d0sc01153c
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author Purushottam, Landa
V. B., Unnikrishnan
Rawale, Dattatraya Gautam
Gujrati, Mansi
Mishra, Surya Dev
T. K., Sajeev
Reddy, Neelesh C.
Adusumalli, Srinivasa Rao
Mishra, Ram Kumar
Rai, Vishal
author_facet Purushottam, Landa
V. B., Unnikrishnan
Rawale, Dattatraya Gautam
Gujrati, Mansi
Mishra, Surya Dev
T. K., Sajeev
Reddy, Neelesh C.
Adusumalli, Srinivasa Rao
Mishra, Ram Kumar
Rai, Vishal
author_sort Purushottam, Landa
collection PubMed
description Analytically pure proteins are indispensable for diverse applications, including therapeutics. Here, we report a methodology where a single amino acid, glycine, enables metal-free protein purification. This robust platform is enabled by a Gly-tag resin for site-specific capture, enrichment, and release through chemically triggered C–C bond dissociation by resonance-assisted electron density polarization.
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spelling pubmed-81631972021-06-04 A single amino acid Gly-tag enables metal-free protein purification Purushottam, Landa V. B., Unnikrishnan Rawale, Dattatraya Gautam Gujrati, Mansi Mishra, Surya Dev T. K., Sajeev Reddy, Neelesh C. Adusumalli, Srinivasa Rao Mishra, Ram Kumar Rai, Vishal Chem Sci Chemistry Analytically pure proteins are indispensable for diverse applications, including therapeutics. Here, we report a methodology where a single amino acid, glycine, enables metal-free protein purification. This robust platform is enabled by a Gly-tag resin for site-specific capture, enrichment, and release through chemically triggered C–C bond dissociation by resonance-assisted electron density polarization. The Royal Society of Chemistry 2020-10-26 /pmc/articles/PMC8163197/ /pubmed/34094495 http://dx.doi.org/10.1039/d0sc01153c Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Purushottam, Landa
V. B., Unnikrishnan
Rawale, Dattatraya Gautam
Gujrati, Mansi
Mishra, Surya Dev
T. K., Sajeev
Reddy, Neelesh C.
Adusumalli, Srinivasa Rao
Mishra, Ram Kumar
Rai, Vishal
A single amino acid Gly-tag enables metal-free protein purification
title A single amino acid Gly-tag enables metal-free protein purification
title_full A single amino acid Gly-tag enables metal-free protein purification
title_fullStr A single amino acid Gly-tag enables metal-free protein purification
title_full_unstemmed A single amino acid Gly-tag enables metal-free protein purification
title_short A single amino acid Gly-tag enables metal-free protein purification
title_sort single amino acid gly-tag enables metal-free protein purification
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163197/
https://www.ncbi.nlm.nih.gov/pubmed/34094495
http://dx.doi.org/10.1039/d0sc01153c
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