A single amino acid Gly-tag enables metal-free protein purification

Analytically pure proteins are indispensable for diverse applications, including therapeutics. Here, we report a methodology where a single amino acid, glycine, enables metal-free protein purification. This robust platform is enabled by a Gly-tag resin for site-specific capture, enrichment, and rele...

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Detalles Bibliográficos
Autores principales: Purushottam, Landa, V. B., Unnikrishnan, Rawale, Dattatraya Gautam, Gujrati, Mansi, Mishra, Surya Dev, T. K., Sajeev, Reddy, Neelesh C., Adusumalli, Srinivasa Rao, Mishra, Ram Kumar, Rai, Vishal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163197/
https://www.ncbi.nlm.nih.gov/pubmed/34094495
http://dx.doi.org/10.1039/d0sc01153c

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