Cargando…

Development and application of ubiquitin-based chemical probes

Protein ubiquitination regulates almost every process in eukaryotic cells. The study of the many enzymes involved in the ubiquitination system and the development of ubiquitination-associated therapeutics are important areas of current research. Synthetic tools such as ubiquitin-based chemical probe...

Descripción completa

Detalles Bibliográficos
Autores principales: Sui, Xin, Wang, Yu, Du, Yun-Xiang, Liang, Lu-Jun, Zheng, Qingyun, Li, Yi-Ming, Liu, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163311/
https://www.ncbi.nlm.nih.gov/pubmed/34123237
http://dx.doi.org/10.1039/d0sc03295f
_version_ 1783700884832649216
author Sui, Xin
Wang, Yu
Du, Yun-Xiang
Liang, Lu-Jun
Zheng, Qingyun
Li, Yi-Ming
Liu, Lei
author_facet Sui, Xin
Wang, Yu
Du, Yun-Xiang
Liang, Lu-Jun
Zheng, Qingyun
Li, Yi-Ming
Liu, Lei
author_sort Sui, Xin
collection PubMed
description Protein ubiquitination regulates almost every process in eukaryotic cells. The study of the many enzymes involved in the ubiquitination system and the development of ubiquitination-associated therapeutics are important areas of current research. Synthetic tools such as ubiquitin-based chemical probes have been making an increasing contribution to deciphering various biochemical components involved in ubiquitin conjugation, recruitment, signaling, and deconjugation. In the present minireview, we summarize the progress of ubiquitin-based chemical probes with an emphasis on their various structures and chemical synthesis. We discuss the utility of the ubiquitin-based chemical probes for discovering and profiling ubiquitin-dependent signaling systems, as well as the monitoring and visualization of ubiquitin-related enzymatic machinery. We also show how the probes can serve to elucidate the molecular mechanism of recognition and catalysis. Collectively, the development and application of ubiquitin-based chemical probes emphasizes the importance and utility of chemical protein synthesis in modern chemical biology.
format Online
Article
Text
id pubmed-8163311
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher The Royal Society of Chemistry
record_format MEDLINE/PubMed
spelling pubmed-81633112021-06-11 Development and application of ubiquitin-based chemical probes Sui, Xin Wang, Yu Du, Yun-Xiang Liang, Lu-Jun Zheng, Qingyun Li, Yi-Ming Liu, Lei Chem Sci Chemistry Protein ubiquitination regulates almost every process in eukaryotic cells. The study of the many enzymes involved in the ubiquitination system and the development of ubiquitination-associated therapeutics are important areas of current research. Synthetic tools such as ubiquitin-based chemical probes have been making an increasing contribution to deciphering various biochemical components involved in ubiquitin conjugation, recruitment, signaling, and deconjugation. In the present minireview, we summarize the progress of ubiquitin-based chemical probes with an emphasis on their various structures and chemical synthesis. We discuss the utility of the ubiquitin-based chemical probes for discovering and profiling ubiquitin-dependent signaling systems, as well as the monitoring and visualization of ubiquitin-related enzymatic machinery. We also show how the probes can serve to elucidate the molecular mechanism of recognition and catalysis. Collectively, the development and application of ubiquitin-based chemical probes emphasizes the importance and utility of chemical protein synthesis in modern chemical biology. The Royal Society of Chemistry 2020-08-04 /pmc/articles/PMC8163311/ /pubmed/34123237 http://dx.doi.org/10.1039/d0sc03295f Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Sui, Xin
Wang, Yu
Du, Yun-Xiang
Liang, Lu-Jun
Zheng, Qingyun
Li, Yi-Ming
Liu, Lei
Development and application of ubiquitin-based chemical probes
title Development and application of ubiquitin-based chemical probes
title_full Development and application of ubiquitin-based chemical probes
title_fullStr Development and application of ubiquitin-based chemical probes
title_full_unstemmed Development and application of ubiquitin-based chemical probes
title_short Development and application of ubiquitin-based chemical probes
title_sort development and application of ubiquitin-based chemical probes
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163311/
https://www.ncbi.nlm.nih.gov/pubmed/34123237
http://dx.doi.org/10.1039/d0sc03295f
work_keys_str_mv AT suixin developmentandapplicationofubiquitinbasedchemicalprobes
AT wangyu developmentandapplicationofubiquitinbasedchemicalprobes
AT duyunxiang developmentandapplicationofubiquitinbasedchemicalprobes
AT lianglujun developmentandapplicationofubiquitinbasedchemicalprobes
AT zhengqingyun developmentandapplicationofubiquitinbasedchemicalprobes
AT liyiming developmentandapplicationofubiquitinbasedchemicalprobes
AT liulei developmentandapplicationofubiquitinbasedchemicalprobes