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Development and application of ubiquitin-based chemical probes
Protein ubiquitination regulates almost every process in eukaryotic cells. The study of the many enzymes involved in the ubiquitination system and the development of ubiquitination-associated therapeutics are important areas of current research. Synthetic tools such as ubiquitin-based chemical probe...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163311/ https://www.ncbi.nlm.nih.gov/pubmed/34123237 http://dx.doi.org/10.1039/d0sc03295f |
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author | Sui, Xin Wang, Yu Du, Yun-Xiang Liang, Lu-Jun Zheng, Qingyun Li, Yi-Ming Liu, Lei |
author_facet | Sui, Xin Wang, Yu Du, Yun-Xiang Liang, Lu-Jun Zheng, Qingyun Li, Yi-Ming Liu, Lei |
author_sort | Sui, Xin |
collection | PubMed |
description | Protein ubiquitination regulates almost every process in eukaryotic cells. The study of the many enzymes involved in the ubiquitination system and the development of ubiquitination-associated therapeutics are important areas of current research. Synthetic tools such as ubiquitin-based chemical probes have been making an increasing contribution to deciphering various biochemical components involved in ubiquitin conjugation, recruitment, signaling, and deconjugation. In the present minireview, we summarize the progress of ubiquitin-based chemical probes with an emphasis on their various structures and chemical synthesis. We discuss the utility of the ubiquitin-based chemical probes for discovering and profiling ubiquitin-dependent signaling systems, as well as the monitoring and visualization of ubiquitin-related enzymatic machinery. We also show how the probes can serve to elucidate the molecular mechanism of recognition and catalysis. Collectively, the development and application of ubiquitin-based chemical probes emphasizes the importance and utility of chemical protein synthesis in modern chemical biology. |
format | Online Article Text |
id | pubmed-8163311 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-81633112021-06-11 Development and application of ubiquitin-based chemical probes Sui, Xin Wang, Yu Du, Yun-Xiang Liang, Lu-Jun Zheng, Qingyun Li, Yi-Ming Liu, Lei Chem Sci Chemistry Protein ubiquitination regulates almost every process in eukaryotic cells. The study of the many enzymes involved in the ubiquitination system and the development of ubiquitination-associated therapeutics are important areas of current research. Synthetic tools such as ubiquitin-based chemical probes have been making an increasing contribution to deciphering various biochemical components involved in ubiquitin conjugation, recruitment, signaling, and deconjugation. In the present minireview, we summarize the progress of ubiquitin-based chemical probes with an emphasis on their various structures and chemical synthesis. We discuss the utility of the ubiquitin-based chemical probes for discovering and profiling ubiquitin-dependent signaling systems, as well as the monitoring and visualization of ubiquitin-related enzymatic machinery. We also show how the probes can serve to elucidate the molecular mechanism of recognition and catalysis. Collectively, the development and application of ubiquitin-based chemical probes emphasizes the importance and utility of chemical protein synthesis in modern chemical biology. The Royal Society of Chemistry 2020-08-04 /pmc/articles/PMC8163311/ /pubmed/34123237 http://dx.doi.org/10.1039/d0sc03295f Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Chemistry Sui, Xin Wang, Yu Du, Yun-Xiang Liang, Lu-Jun Zheng, Qingyun Li, Yi-Ming Liu, Lei Development and application of ubiquitin-based chemical probes |
title | Development and application of ubiquitin-based chemical probes |
title_full | Development and application of ubiquitin-based chemical probes |
title_fullStr | Development and application of ubiquitin-based chemical probes |
title_full_unstemmed | Development and application of ubiquitin-based chemical probes |
title_short | Development and application of ubiquitin-based chemical probes |
title_sort | development and application of ubiquitin-based chemical probes |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163311/ https://www.ncbi.nlm.nih.gov/pubmed/34123237 http://dx.doi.org/10.1039/d0sc03295f |
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