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Triazole-based osmium(ii) complexes displaying red/near-IR luminescence: antimicrobial activity and super-resolution imaging

Cellular uptake, luminescence imaging and antimicrobial activity against clinically relevant methicillin-resistant S. aureus (MRSA) bacteria are reported. The osmium(ii) complexes [Os(N^N)(3)](2+) (N^N = 1-benzyl-4-(pyrid-2-yl)-1,2,3-triazole (1(2+)); 1-benzyl-4-(pyrimidin-2-yl)-1,2,3-triazole (2(2+...

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Detalles Bibliográficos
Autores principales: Smitten, Kirsty L., Scattergood, Paul A., Kiker, Charlotte, Thomas, Jim A., Elliott, Paul I. P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163367/
https://www.ncbi.nlm.nih.gov/pubmed/34123147
http://dx.doi.org/10.1039/d0sc03563g
Descripción
Sumario:Cellular uptake, luminescence imaging and antimicrobial activity against clinically relevant methicillin-resistant S. aureus (MRSA) bacteria are reported. The osmium(ii) complexes [Os(N^N)(3)](2+) (N^N = 1-benzyl-4-(pyrid-2-yl)-1,2,3-triazole (1(2+)); 1-benzyl-4-(pyrimidin-2-yl)-1,2,3-triazole (2(2+)); 1-benzyl-4-(pyrazin-2-yl)-1,2,3-triazole (3(2+))) were prepared and isolated as the chloride salts of their meridional and facial isomers. The complexes display prominent spin-forbidden ground state to triplet metal-to-ligand charge transfer ((3)MLCT) state absorption bands enabling excitation as low as 600 nm for fac/mer-3(2+) and observation of emission in aqueous solution in the deep-red/near-IR regions of the spectrum. Cellular uptake studies within MRSA cells show antimicrobial activity for 1(2+) and 2(2+) with greater toxicity for the meridional isomers in each case and mer-1(2+) showing the greatest potency (32 μg mL(−1) in defined minimal media). Super-resolution imaging experiments demonstrate binding of mer- and fac-1(2+) to bacterial DNA with high Pearson's colocalisation coefficients (up to 0.95 using DAPI). Phototoxicity studies showed the complexes exhibited a higher antimicrobial activity upon irradiation with light.