Super-resolution RESOLFT microscopy of lipid bilayers using a fluorophore-switch dyad

Dyads consisting of a photochromic switch covalently linked to a fluorescent dye allow the emission from the dye to be controlled by reversible photoisomerization of the switch; one form of the switch quenches fluorescence by accepting energy from the dye. Here we investigate the use of dyads of thi...

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Detalles Bibliográficos
Autores principales: Frawley, Andrew T., Wycisk, Virginia, Xiong, Yaoyao, Galiani, Silvia, Sezgin, Erdinc, Urbančič, Iztok, Vargas Jentzsch, Andreas, Leslie, Kathryn G., Eggeling, Christian, Anderson, Harry L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163400/
https://www.ncbi.nlm.nih.gov/pubmed/34123149
http://dx.doi.org/10.1039/d0sc02447c
Descripción
Sumario:Dyads consisting of a photochromic switch covalently linked to a fluorescent dye allow the emission from the dye to be controlled by reversible photoisomerization of the switch; one form of the switch quenches fluorescence by accepting energy from the dye. Here we investigate the use of dyads of this type for super-resolution imaging of lipid bilayers. Giant unilamellar vesicles stained with the dyads were imaged with about a two-fold resolution-enhancement compared with conventional confocal microscopy. This was achieved by exciting the fluorophore at 594 nm, using a switch activated by violet and red light (405/640 nm).

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