Super-resolution RESOLFT microscopy of lipid bilayers using a fluorophore-switch dyad

Dyads consisting of a photochromic switch covalently linked to a fluorescent dye allow the emission from the dye to be controlled by reversible photoisomerization of the switch; one form of the switch quenches fluorescence by accepting energy from the dye. Here we investigate the use of dyads of thi...

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Autores principales: Frawley, Andrew T., Wycisk, Virginia, Xiong, Yaoyao, Galiani, Silvia, Sezgin, Erdinc, Urbančič, Iztok, Vargas Jentzsch, Andreas, Leslie, Kathryn G., Eggeling, Christian, Anderson, Harry L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163400/
https://www.ncbi.nlm.nih.gov/pubmed/34123149
http://dx.doi.org/10.1039/d0sc02447c
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author Frawley, Andrew T.
Wycisk, Virginia
Xiong, Yaoyao
Galiani, Silvia
Sezgin, Erdinc
Urbančič, Iztok
Vargas Jentzsch, Andreas
Leslie, Kathryn G.
Eggeling, Christian
Anderson, Harry L.
author_facet Frawley, Andrew T.
Wycisk, Virginia
Xiong, Yaoyao
Galiani, Silvia
Sezgin, Erdinc
Urbančič, Iztok
Vargas Jentzsch, Andreas
Leslie, Kathryn G.
Eggeling, Christian
Anderson, Harry L.
author_sort Frawley, Andrew T.
collection PubMed
description Dyads consisting of a photochromic switch covalently linked to a fluorescent dye allow the emission from the dye to be controlled by reversible photoisomerization of the switch; one form of the switch quenches fluorescence by accepting energy from the dye. Here we investigate the use of dyads of this type for super-resolution imaging of lipid bilayers. Giant unilamellar vesicles stained with the dyads were imaged with about a two-fold resolution-enhancement compared with conventional confocal microscopy. This was achieved by exciting the fluorophore at 594 nm, using a switch activated by violet and red light (405/640 nm).
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spelling pubmed-81634002021-06-11 Super-resolution RESOLFT microscopy of lipid bilayers using a fluorophore-switch dyad Frawley, Andrew T. Wycisk, Virginia Xiong, Yaoyao Galiani, Silvia Sezgin, Erdinc Urbančič, Iztok Vargas Jentzsch, Andreas Leslie, Kathryn G. Eggeling, Christian Anderson, Harry L. Chem Sci Chemistry Dyads consisting of a photochromic switch covalently linked to a fluorescent dye allow the emission from the dye to be controlled by reversible photoisomerization of the switch; one form of the switch quenches fluorescence by accepting energy from the dye. Here we investigate the use of dyads of this type for super-resolution imaging of lipid bilayers. Giant unilamellar vesicles stained with the dyads were imaged with about a two-fold resolution-enhancement compared with conventional confocal microscopy. This was achieved by exciting the fluorophore at 594 nm, using a switch activated by violet and red light (405/640 nm). The Royal Society of Chemistry 2020-08-10 /pmc/articles/PMC8163400/ /pubmed/34123149 http://dx.doi.org/10.1039/d0sc02447c Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Frawley, Andrew T.
Wycisk, Virginia
Xiong, Yaoyao
Galiani, Silvia
Sezgin, Erdinc
Urbančič, Iztok
Vargas Jentzsch, Andreas
Leslie, Kathryn G.
Eggeling, Christian
Anderson, Harry L.
Super-resolution RESOLFT microscopy of lipid bilayers using a fluorophore-switch dyad
title Super-resolution RESOLFT microscopy of lipid bilayers using a fluorophore-switch dyad
title_full Super-resolution RESOLFT microscopy of lipid bilayers using a fluorophore-switch dyad
title_fullStr Super-resolution RESOLFT microscopy of lipid bilayers using a fluorophore-switch dyad
title_full_unstemmed Super-resolution RESOLFT microscopy of lipid bilayers using a fluorophore-switch dyad
title_short Super-resolution RESOLFT microscopy of lipid bilayers using a fluorophore-switch dyad
title_sort super-resolution resolft microscopy of lipid bilayers using a fluorophore-switch dyad
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163400/
https://www.ncbi.nlm.nih.gov/pubmed/34123149
http://dx.doi.org/10.1039/d0sc02447c
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