Cargando…

[Gly14]-Humanin Ameliorates High Glucose-Induced Apoptosis by Inhibiting the Expression of MicroRNA-155 in Endothelial Microparticles

BACKGROUND: Humanin, a newly emerging endogenously expressed cytoprotective peptide, has been shown to have anti-apoptotic properties effects by protecting neuronal cells injury. Endothelial microparticles (EMPs) are considered as vital mediators in intercellular communication. EMPs may regulate var...

Descripción completa

Detalles Bibliográficos
Autores principales: Shen, Meng-Yuan, Wang, Miao, Liu, Zhihua, Wang, Shurong, Xie, Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163639/
https://www.ncbi.nlm.nih.gov/pubmed/34079312
http://dx.doi.org/10.2147/DMSO.S306026
_version_ 1783700947924418560
author Shen, Meng-Yuan
Wang, Miao
Liu, Zhihua
Wang, Shurong
Xie, Ying
author_facet Shen, Meng-Yuan
Wang, Miao
Liu, Zhihua
Wang, Shurong
Xie, Ying
author_sort Shen, Meng-Yuan
collection PubMed
description BACKGROUND: Humanin, a newly emerging endogenously expressed cytoprotective peptide, has been shown to have anti-apoptotic properties effects by protecting neuronal cells injury. Endothelial microparticles (EMPs) are considered as vital mediators in intercellular communication. EMPs may regulate various physiological and pathological processes by transferring mRNAs and microRNAs (miRNAs) to recipient cells. METHODS: EMPs were isolated from human umbilical vein endothelial cells (HUVECs) by ultracentrifugation. EMPs were characterized by transmission electron microscopy and nanoparticle tracking analyses. Observation of EMPs uptake into HUVECs and the number of EMPs were realized by confocal microscopy. The expression of miR-155 was examined using real-time PCR. Cell apoptosis was examined by flow cytometry assay. RESULTS: We found that high glucose (HG) increased the number of EMPs and upregulated the expression of miR-155 contained within EMPs, which was mitigated by HNG pretreatment. miR-155 overexpression in EMPs reversed the effects of HNG pretreatment and increased apoptosis of target cells. Effects of HNG pretreatment on HG-treated endothelial cells (ECs) were mitigated after miR-155 mimic transfection into HUVECs while were augmented after miR-155 inhibitor transfection into HUVECs. CONCLUSION: HNG inhibited HG-induced apoptosis of ECs and the effect of HNG may be mediated by inhibiting the transfer of EMPs miR-155 from HG-induced HUVECs to normal cells. This study provides a new direction for biological products related to humanin to treat vascular complications associated with all forms of diabetes mellitus.
format Online
Article
Text
id pubmed-8163639
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Dove
record_format MEDLINE/PubMed
spelling pubmed-81636392021-06-01 [Gly14]-Humanin Ameliorates High Glucose-Induced Apoptosis by Inhibiting the Expression of MicroRNA-155 in Endothelial Microparticles Shen, Meng-Yuan Wang, Miao Liu, Zhihua Wang, Shurong Xie, Ying Diabetes Metab Syndr Obes Original Research BACKGROUND: Humanin, a newly emerging endogenously expressed cytoprotective peptide, has been shown to have anti-apoptotic properties effects by protecting neuronal cells injury. Endothelial microparticles (EMPs) are considered as vital mediators in intercellular communication. EMPs may regulate various physiological and pathological processes by transferring mRNAs and microRNAs (miRNAs) to recipient cells. METHODS: EMPs were isolated from human umbilical vein endothelial cells (HUVECs) by ultracentrifugation. EMPs were characterized by transmission electron microscopy and nanoparticle tracking analyses. Observation of EMPs uptake into HUVECs and the number of EMPs were realized by confocal microscopy. The expression of miR-155 was examined using real-time PCR. Cell apoptosis was examined by flow cytometry assay. RESULTS: We found that high glucose (HG) increased the number of EMPs and upregulated the expression of miR-155 contained within EMPs, which was mitigated by HNG pretreatment. miR-155 overexpression in EMPs reversed the effects of HNG pretreatment and increased apoptosis of target cells. Effects of HNG pretreatment on HG-treated endothelial cells (ECs) were mitigated after miR-155 mimic transfection into HUVECs while were augmented after miR-155 inhibitor transfection into HUVECs. CONCLUSION: HNG inhibited HG-induced apoptosis of ECs and the effect of HNG may be mediated by inhibiting the transfer of EMPs miR-155 from HG-induced HUVECs to normal cells. This study provides a new direction for biological products related to humanin to treat vascular complications associated with all forms of diabetes mellitus. Dove 2021-05-24 /pmc/articles/PMC8163639/ /pubmed/34079312 http://dx.doi.org/10.2147/DMSO.S306026 Text en © 2021 Shen et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Shen, Meng-Yuan
Wang, Miao
Liu, Zhihua
Wang, Shurong
Xie, Ying
[Gly14]-Humanin Ameliorates High Glucose-Induced Apoptosis by Inhibiting the Expression of MicroRNA-155 in Endothelial Microparticles
title [Gly14]-Humanin Ameliorates High Glucose-Induced Apoptosis by Inhibiting the Expression of MicroRNA-155 in Endothelial Microparticles
title_full [Gly14]-Humanin Ameliorates High Glucose-Induced Apoptosis by Inhibiting the Expression of MicroRNA-155 in Endothelial Microparticles
title_fullStr [Gly14]-Humanin Ameliorates High Glucose-Induced Apoptosis by Inhibiting the Expression of MicroRNA-155 in Endothelial Microparticles
title_full_unstemmed [Gly14]-Humanin Ameliorates High Glucose-Induced Apoptosis by Inhibiting the Expression of MicroRNA-155 in Endothelial Microparticles
title_short [Gly14]-Humanin Ameliorates High Glucose-Induced Apoptosis by Inhibiting the Expression of MicroRNA-155 in Endothelial Microparticles
title_sort [gly14]-humanin ameliorates high glucose-induced apoptosis by inhibiting the expression of microrna-155 in endothelial microparticles
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163639/
https://www.ncbi.nlm.nih.gov/pubmed/34079312
http://dx.doi.org/10.2147/DMSO.S306026
work_keys_str_mv AT shenmengyuan gly14humaninameliorateshighglucoseinducedapoptosisbyinhibitingtheexpressionofmicrorna155inendothelialmicroparticles
AT wangmiao gly14humaninameliorateshighglucoseinducedapoptosisbyinhibitingtheexpressionofmicrorna155inendothelialmicroparticles
AT liuzhihua gly14humaninameliorateshighglucoseinducedapoptosisbyinhibitingtheexpressionofmicrorna155inendothelialmicroparticles
AT wangshurong gly14humaninameliorateshighglucoseinducedapoptosisbyinhibitingtheexpressionofmicrorna155inendothelialmicroparticles
AT xieying gly14humaninameliorateshighglucoseinducedapoptosisbyinhibitingtheexpressionofmicrorna155inendothelialmicroparticles