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DNA barcodes of birds from northern Colombia
DNA barcode datasets are a useful tool for conservation and aid in taxonomic identification, particularly in megadiverse tropical countries seeking to document and describe its biota, which is dropping at an alarming rate during recent decades. Here we report the barcodes for several low elevation b...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Pensoft Publishers
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163714/ https://www.ncbi.nlm.nih.gov/pubmed/34084068 http://dx.doi.org/10.3897/BDJ.9.e64842 |
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author | Pulgarín-R, Paulo Cesar Olivera-Angel, Martha Ortíz, Luisa Nanclares, Duván Velásquez-Restrepo, Sara Díaz-Nieto, Juan Fernando |
author_facet | Pulgarín-R, Paulo Cesar Olivera-Angel, Martha Ortíz, Luisa Nanclares, Duván Velásquez-Restrepo, Sara Díaz-Nieto, Juan Fernando |
author_sort | Pulgarín-R, Paulo Cesar |
collection | PubMed |
description | DNA barcode datasets are a useful tool for conservation and aid in taxonomic identification, particularly in megadiverse tropical countries seeking to document and describe its biota, which is dropping at an alarming rate during recent decades. Here we report the barcodes for several low elevation bird species from northern Colombia with the goal to provide tools for species identification in this region of South America. We blood-sampled birds in a lowland tropical forest with various degrees of intervention using standard 3 × 12 m mist-nets. We extracted DNA and sequenced the COI barcode gene using standard primers and laboratory methods. We obtained 26 COI sequences from 18 species, 10 families and three orders and found that barcodes largely matched (but not always) phenotypic identification (> 90%) and they also facilitated the identification of several challenging passerine species. Despite our reduced sampling, our study represents the first attempt to document COI barcodes for birds (from blood samples) in this part of Colombia, which fills a considerable gap of sampling in this part of South America. |
format | Online Article Text |
id | pubmed-8163714 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Pensoft Publishers |
record_format | MEDLINE/PubMed |
spelling | pubmed-81637142021-06-02 DNA barcodes of birds from northern Colombia Pulgarín-R, Paulo Cesar Olivera-Angel, Martha Ortíz, Luisa Nanclares, Duván Velásquez-Restrepo, Sara Díaz-Nieto, Juan Fernando Biodivers Data J Research Article DNA barcode datasets are a useful tool for conservation and aid in taxonomic identification, particularly in megadiverse tropical countries seeking to document and describe its biota, which is dropping at an alarming rate during recent decades. Here we report the barcodes for several low elevation bird species from northern Colombia with the goal to provide tools for species identification in this region of South America. We blood-sampled birds in a lowland tropical forest with various degrees of intervention using standard 3 × 12 m mist-nets. We extracted DNA and sequenced the COI barcode gene using standard primers and laboratory methods. We obtained 26 COI sequences from 18 species, 10 families and three orders and found that barcodes largely matched (but not always) phenotypic identification (> 90%) and they also facilitated the identification of several challenging passerine species. Despite our reduced sampling, our study represents the first attempt to document COI barcodes for birds (from blood samples) in this part of Colombia, which fills a considerable gap of sampling in this part of South America. Pensoft Publishers 2021-05-21 /pmc/articles/PMC8163714/ /pubmed/34084068 http://dx.doi.org/10.3897/BDJ.9.e64842 Text en Paulo Cesar Pulgarín-R, Martha Olivera-Angel, Luisa Ortíz, Duván Nanclares, Sara Velásquez-Restrepo, Juan Fernando Díaz-Nieto https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Pulgarín-R, Paulo Cesar Olivera-Angel, Martha Ortíz, Luisa Nanclares, Duván Velásquez-Restrepo, Sara Díaz-Nieto, Juan Fernando DNA barcodes of birds from northern Colombia |
title | DNA barcodes of birds from northern Colombia |
title_full | DNA barcodes of birds from northern Colombia |
title_fullStr | DNA barcodes of birds from northern Colombia |
title_full_unstemmed | DNA barcodes of birds from northern Colombia |
title_short | DNA barcodes of birds from northern Colombia |
title_sort | dna barcodes of birds from northern colombia |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163714/ https://www.ncbi.nlm.nih.gov/pubmed/34084068 http://dx.doi.org/10.3897/BDJ.9.e64842 |
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