Amino acid residue at the 165th position tunes EYFP chromophore maturation. A structure-based design

For the whole GFP family, a few cases, when a single mutation in the chromophore environment strongly inhibits maturation, were described. Here we study EYFP-F165G – a variant of the enhanced yellow fluorescent protein – obtained by a single F165G replacement, and demonstrated multiple fluorescent s...

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Detalles Bibliográficos
Autores principales: Pletneva, Nadya V., Maksimov, Eugene G., Protasova, Elena A., Mamontova, Anastasia V., Simonyan, Tatiana R., Ziganshin, Rustam H., Lukyanov, Konstantin A., Muslinkina, Liya, Pletnev, Sergei, Bogdanov, Alexey M., Pletnev, Vladimir Z.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Research Network of Computational and Structural Biotechnology 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163865/
https://www.ncbi.nlm.nih.gov/pubmed/34136094
http://dx.doi.org/10.1016/j.csbj.2021.05.017

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163865/
https://www.ncbi.nlm.nih.gov/pubmed/34136094
http://dx.doi.org/10.1016/j.csbj.2021.05.017

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