Cargando…

Effects of cofD gene knock-out on the methanogenesis of Methanobrevibacter ruminantium

This study aimed to investigate the effects of cofD gene knock-out on the synthesis of coenzyme F(420) and production of methane in Methanobrevibacter ruminantium (M. ruminantium). The experiment successfully constructed a cofD gene knock-out M. ruminantium via homologous recombination technology. T...

Descripción completa

Detalles Bibliográficos
Autores principales: Ma, Jian, Wang, Xueying, Zhou, Ting, Hu, Rui, Zou, Huawei, Wang, Zhisheng, Tan, Cui, Zhang, Xiangfei, Peng, Quanhui, Xue, Bai, Wang, Lizhi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163928/
https://www.ncbi.nlm.nih.gov/pubmed/34047886
http://dx.doi.org/10.1186/s13568-021-01236-2
_version_ 1783701007076687872
author Ma, Jian
Wang, Xueying
Zhou, Ting
Hu, Rui
Zou, Huawei
Wang, Zhisheng
Tan, Cui
Zhang, Xiangfei
Peng, Quanhui
Xue, Bai
Wang, Lizhi
author_facet Ma, Jian
Wang, Xueying
Zhou, Ting
Hu, Rui
Zou, Huawei
Wang, Zhisheng
Tan, Cui
Zhang, Xiangfei
Peng, Quanhui
Xue, Bai
Wang, Lizhi
author_sort Ma, Jian
collection PubMed
description This study aimed to investigate the effects of cofD gene knock-out on the synthesis of coenzyme F(420) and production of methane in Methanobrevibacter ruminantium (M. ruminantium). The experiment successfully constructed a cofD gene knock-out M. ruminantium via homologous recombination technology. The results showed that the logarithmic phase of mutant M. ruminantium (12 h) was lower than the wild-type (24 h). The maximum biomass and specific growth rate of mutant M. ruminantium were significantly lower (P < 0.05) than those of wild-type, and the maximum biomass of mutant M. ruminantium was approximately half of the wild-type; meanwhile, the proliferation was reduced. The synthesis amount of coenzyme F(420) of M. ruminantium was significantly decreased (P < 0.05) after the cofD gene knock-out. Moreover, the maximum amount of H(2) consumed and CH(4) produced by mutant were 14 and 2% of wild-type M. ruminantium respectively. In conclusion, cofD gene knock-out induced the decreased growth rate and reproductive ability of M. ruminantium. Subsequently, the synthesis of coenzyme F(420) was decreased. Ultimately, the production capacity of CH(4) in M. ruminantium was reduced. Our research provides evidence that cofD gene plays an indispensable role in the regulation of coenzyme F(420) synthesis and CH(4) production in M. ruminantium. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13568-021-01236-2.
format Online
Article
Text
id pubmed-8163928
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Springer Berlin Heidelberg
record_format MEDLINE/PubMed
spelling pubmed-81639282021-06-17 Effects of cofD gene knock-out on the methanogenesis of Methanobrevibacter ruminantium Ma, Jian Wang, Xueying Zhou, Ting Hu, Rui Zou, Huawei Wang, Zhisheng Tan, Cui Zhang, Xiangfei Peng, Quanhui Xue, Bai Wang, Lizhi AMB Express Original Article This study aimed to investigate the effects of cofD gene knock-out on the synthesis of coenzyme F(420) and production of methane in Methanobrevibacter ruminantium (M. ruminantium). The experiment successfully constructed a cofD gene knock-out M. ruminantium via homologous recombination technology. The results showed that the logarithmic phase of mutant M. ruminantium (12 h) was lower than the wild-type (24 h). The maximum biomass and specific growth rate of mutant M. ruminantium were significantly lower (P < 0.05) than those of wild-type, and the maximum biomass of mutant M. ruminantium was approximately half of the wild-type; meanwhile, the proliferation was reduced. The synthesis amount of coenzyme F(420) of M. ruminantium was significantly decreased (P < 0.05) after the cofD gene knock-out. Moreover, the maximum amount of H(2) consumed and CH(4) produced by mutant were 14 and 2% of wild-type M. ruminantium respectively. In conclusion, cofD gene knock-out induced the decreased growth rate and reproductive ability of M. ruminantium. Subsequently, the synthesis of coenzyme F(420) was decreased. Ultimately, the production capacity of CH(4) in M. ruminantium was reduced. Our research provides evidence that cofD gene plays an indispensable role in the regulation of coenzyme F(420) synthesis and CH(4) production in M. ruminantium. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13568-021-01236-2. Springer Berlin Heidelberg 2021-05-28 /pmc/articles/PMC8163928/ /pubmed/34047886 http://dx.doi.org/10.1186/s13568-021-01236-2 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Ma, Jian
Wang, Xueying
Zhou, Ting
Hu, Rui
Zou, Huawei
Wang, Zhisheng
Tan, Cui
Zhang, Xiangfei
Peng, Quanhui
Xue, Bai
Wang, Lizhi
Effects of cofD gene knock-out on the methanogenesis of Methanobrevibacter ruminantium
title Effects of cofD gene knock-out on the methanogenesis of Methanobrevibacter ruminantium
title_full Effects of cofD gene knock-out on the methanogenesis of Methanobrevibacter ruminantium
title_fullStr Effects of cofD gene knock-out on the methanogenesis of Methanobrevibacter ruminantium
title_full_unstemmed Effects of cofD gene knock-out on the methanogenesis of Methanobrevibacter ruminantium
title_short Effects of cofD gene knock-out on the methanogenesis of Methanobrevibacter ruminantium
title_sort effects of cofd gene knock-out on the methanogenesis of methanobrevibacter ruminantium
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163928/
https://www.ncbi.nlm.nih.gov/pubmed/34047886
http://dx.doi.org/10.1186/s13568-021-01236-2
work_keys_str_mv AT majian effectsofcofdgeneknockoutonthemethanogenesisofmethanobrevibacterruminantium
AT wangxueying effectsofcofdgeneknockoutonthemethanogenesisofmethanobrevibacterruminantium
AT zhouting effectsofcofdgeneknockoutonthemethanogenesisofmethanobrevibacterruminantium
AT hurui effectsofcofdgeneknockoutonthemethanogenesisofmethanobrevibacterruminantium
AT zouhuawei effectsofcofdgeneknockoutonthemethanogenesisofmethanobrevibacterruminantium
AT wangzhisheng effectsofcofdgeneknockoutonthemethanogenesisofmethanobrevibacterruminantium
AT tancui effectsofcofdgeneknockoutonthemethanogenesisofmethanobrevibacterruminantium
AT zhangxiangfei effectsofcofdgeneknockoutonthemethanogenesisofmethanobrevibacterruminantium
AT pengquanhui effectsofcofdgeneknockoutonthemethanogenesisofmethanobrevibacterruminantium
AT xuebai effectsofcofdgeneknockoutonthemethanogenesisofmethanobrevibacterruminantium
AT wanglizhi effectsofcofdgeneknockoutonthemethanogenesisofmethanobrevibacterruminantium