A dual cardiomyocyte reporter model derived from human pluripotent stem cells

Cardiovascular diseases (CVD) remain the leading cause of death in the USA. Cardiomyocytes (CMs) derived from human pluripotent stem cells (hPSCs) provide a valuable cell source for regenerative therapy, disease modeling, and drug screening. Here, we established a hPSC line integrated with a mCherry...

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Detalles Bibliográficos
Autores principales: Jiang, Yuqian, Bao, Xiaoping, Lian, Xiaojun Lance
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164304/
https://www.ncbi.nlm.nih.gov/pubmed/34051863
http://dx.doi.org/10.1186/s13287-021-02341-6
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author Jiang, Yuqian
Bao, Xiaoping
Lian, Xiaojun Lance
author_facet Jiang, Yuqian
Bao, Xiaoping
Lian, Xiaojun Lance
author_sort Jiang, Yuqian
collection PubMed
description Cardiovascular diseases (CVD) remain the leading cause of death in the USA. Cardiomyocytes (CMs) derived from human pluripotent stem cells (hPSCs) provide a valuable cell source for regenerative therapy, disease modeling, and drug screening. Here, we established a hPSC line integrated with a mCherry fluorescent protein driven by the alpha myosin heavy chain (aMHC) promoter, which could be used to purify CMs based on the aMHC promoter activity in these cells. Combined with a fluorescent voltage indicator, ASAP2f, we achieved a dual reporter CM platform, which enables purification and characterization of CM subtypes and holds great potential for disease modeling and drug discovery of CVD. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13287-021-02341-6.
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spelling pubmed-81643042021-06-01 A dual cardiomyocyte reporter model derived from human pluripotent stem cells Jiang, Yuqian Bao, Xiaoping Lian, Xiaojun Lance Stem Cell Res Ther Short Report Cardiovascular diseases (CVD) remain the leading cause of death in the USA. Cardiomyocytes (CMs) derived from human pluripotent stem cells (hPSCs) provide a valuable cell source for regenerative therapy, disease modeling, and drug screening. Here, we established a hPSC line integrated with a mCherry fluorescent protein driven by the alpha myosin heavy chain (aMHC) promoter, which could be used to purify CMs based on the aMHC promoter activity in these cells. Combined with a fluorescent voltage indicator, ASAP2f, we achieved a dual reporter CM platform, which enables purification and characterization of CM subtypes and holds great potential for disease modeling and drug discovery of CVD. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13287-021-02341-6. BioMed Central 2021-05-29 /pmc/articles/PMC8164304/ /pubmed/34051863 http://dx.doi.org/10.1186/s13287-021-02341-6 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Short Report
Jiang, Yuqian
Bao, Xiaoping
Lian, Xiaojun Lance
A dual cardiomyocyte reporter model derived from human pluripotent stem cells
title A dual cardiomyocyte reporter model derived from human pluripotent stem cells
title_full A dual cardiomyocyte reporter model derived from human pluripotent stem cells
title_fullStr A dual cardiomyocyte reporter model derived from human pluripotent stem cells
title_full_unstemmed A dual cardiomyocyte reporter model derived from human pluripotent stem cells
title_short A dual cardiomyocyte reporter model derived from human pluripotent stem cells
title_sort dual cardiomyocyte reporter model derived from human pluripotent stem cells
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164304/
https://www.ncbi.nlm.nih.gov/pubmed/34051863
http://dx.doi.org/10.1186/s13287-021-02341-6
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