Cargando…

SARS-CoV-2 RNA Quantification Using Droplet Digital RT-PCR

Quantitative viral load assays have transformed our understanding of viral diseases. They hold similar potential to advance COVID-19 control and prevention, but SARS-CoV-2 viral load tests are not yet widely available. SARS-CoV-2 molecular diagnostic tests, which typically employ real-time RT-PCR, y...

Descripción completa

Detalles Bibliográficos
Autores principales:	Kinloch, Natalie N., Ritchie, Gordon, Dong, Winnie, Cobarrubias, Kyle D., Sudderuddin, Hanwei, Lawson, Tanya, Matic, Nancy, Montaner, Julio S.G., Leung, Victor, Romney, Marc G., Lowe, Christopher F., Brumme, Chanson J., Brumme, Zabrina L.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Association for Molecular Pathology and American Society for Investigative Pathology. Published by Elsevier Inc. 2021
Materias:	Regular Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164350/ https://www.ncbi.nlm.nih.gov/pubmed/34062285 http://dx.doi.org/10.1016/j.jmoldx.2021.04.014

Ejemplares similares

Evaluation of Nasopharyngeal Swab Collection Techniques for Nucleic Acid Recovery and Participant Experience: Recommendations for COVID-19 Diagnostics
por: Kinloch, Natalie N, et al.
Publicado: (2020)

Rapid Detection of SARS-CoV-2 Variants of Concern, Including B.1.1.28/P.1, British Columbia, Canada
por: Matic, Nancy, et al.
Publicado: (2021)

Longitudinal within-host evolution of HIV Nef-mediated CD4, HLA and SERINC5 downregulation activity: a case study
por: Sudderuddin, Hanwei, et al.
Publicado: (2020)

Proviral Turnover During Untreated HIV Infection Is Dynamic and Variable Between Hosts, Impacting Reservoir Composition on ART
por: Brooks, Kelsie, et al.
Publicado: (2021)

Detection of HIV transmission hotspots in British Columbia, Canada: A novel framework for the prioritization and allocation of treatment and prevention resources
por: McLaughlin, Angela, et al.
Publicado: (2019)

High Levels of Dual-Class Drug Resistance in HIV-Infected Children Failing First-Line Antiretroviral Therapy in Southern Ethiopia
por: Tadesse, Birkneh Tilahun, et al.
Publicado: (2018)

Effects of COVID-19 mRNA vaccination on HIV viremia and reservoir size
por: DUNCAN, Maggie C., et al.
Publicado: (2023)

HIV reservoirs are dominated by genetically younger and clonally enriched proviruses
por: Kinloch, Natalie N., et al.
Publicado: (2023)

Concordance of HIV transmission risk factors elucidated using viral diversification rate and phylogenetic clustering
por: McLaughlin, Angela, et al.
Publicado: (2021)

HIV Proviral Burden, Genetic Diversity, and Dynamics in Viremic Controllers Who Subsequently Initiated Suppressive Antiretroviral Therapy
por: Omondi, F. Harrison, et al.
Publicado: (2021)

Estimation of measurement error in plasma HIV-1 RNA assays near their limit of quantification
por: Lima, Viviane D., et al.
Publicado: (2017)

Weak humoral immune reactivity among residents of long-term care facilities following one dose of the BNT162b2 mRNA COVID-19 vaccine
por: Brockman, Mark A., et al.
Publicado: (2021)

Serial infection with SARS-CoV-2 Omicron BA.1 and BA.2 following three-dose COVID-19 vaccination
por: Lapointe, Hope R., et al.
Publicado: (2022)

HIV proviral genetic diversity, compartmentalization and inferred dynamics in lung and blood during long-term suppressive antiretroviral therapy
por: Shahid, Aniqa, et al.
Publicado: (2022)

SARS-CoV-2 live virus neutralization after four COVID-19 vaccine doses in people with HIV receiving suppressive antiretroviral therapy
por: Cheung, Peter K., et al.
Publicado: (2023)

Lack of a significant impact of Gag-Protease-mediated HIV-1 replication capacity on clinical parameters in treatment-naive Japanese individuals
por: Sakai, Keiko, et al.
Publicado: (2015)

Erratum to: Lack of a significant impact of Gag-Protease-mediated HIV-1 replication capacity on clinical parameters in treatment-naive Japanese individuals
por: Sakai, Keiko, et al.
Publicado: (2015)

Extensive host immune adaptation in a concentrated North American HIV epidemic
por: Brumme, Zabrina L., et al.
Publicado: (2018)

Impact of Age and Severe Acute Respiratory Syndrome Coronavirus 2 Breakthrough Infection on Humoral Immune Responses After Three Doses of Coronavirus Disease 2019 mRNA Vaccine
por: Mwimanzi, Francis, et al.
Publicado: (2023)

Sociodemographic correlates of HIV drug resistance and access to drug resistance testing in British Columbia, Canada
por: Rocheleau, Genevieve, et al.
Publicado: (2017)

Rates and Correlates of Short Term Virologic Response among Treatment-Naïve HIV-Infected Children Initiating Antiretroviral Therapy in Ethiopia: A Multi-Center Prospective Cohort Study
por: Tadesse, Birkneh Tilahun, et al.
Publicado: (2019)

1776. Step-Wise Algorithm for the Detection of Respiratory Viruses: Integrating a Rapid Influenza A/B and RSV PCR with a Multiplex Respiratory Virus Panel to Target High-Risk Patient Populations
por: Matic, Nancy, et al.
Publicado: (2019)

Older adults mount less durable humoral responses to two doses of COVID-19 mRNA vaccine, but strong initial responses to a third dose
por: Mwimanzi, Francis, et al.
Publicado: (2022)

Detection of low levels of SARS-CoV-2 RNA from nasopharyngeal swabs using three commercial molecular assays
por: Lowe, Christopher F., et al.
Publicado: (2020)

Automated molecular testing of saliva for SARS-CoV-2 detection
por: Matic, Nancy, et al.
Publicado: (2021)

T-cell responses to sequentially emerging viral escape mutants shape long-term HIV-1 population dynamics
por: Akahoshi, Tomohiro, et al.
Publicado: (2020)

Omicron (B.1.1.529) SARS-CoV-2 viral load among nasopharyngeal and oral samples compared to other variants of concern and impact on diagnostic testing strategy
por: Matic, Nancy, et al.
Publicado: (2022)

Tracking the culprit: HIV-1 evolution and immune selection revealed by single-genome amplification
por: Brumme, Zabrina L., et al.
Publicado: (2009)

Molecular Epidemiology of HIV-1 in Ghana: Subtype Distribution, Drug Resistance and Coreceptor Usage
por: Appah, Anna, et al.
Publicado: (2022)

Practical challenges to the clinical implementation of saliva for SARS-CoV-2 detection
por: Matic, Nancy, et al.
Publicado: (2020)

Correction: Evidence of Differential HLA Class I-Mediated Viral Evolution in Functional and Accessory/Regulatory Genes of HIV-1
por: Brumme, Zabrina L, et al.
Publicado: (2007)

Evidence of Differential HLA Class I-Mediated Viral Evolution in Functional and Accessory/Regulatory Genes of HIV-1
por: Brumme, Zabrina L, et al.
Publicado: (2007)

PP 1.37 – 00186 Investigating Short-Term Effects of COVID-19 mRNA Vaccination on Plasma Viremia and Intact HIV Reservoir Size in Individuals Receiving Antiretroviral Therapy (ART)
por: Duncan, M.C., et al.
Publicado: (2022)

Characteristics and Outcomes of Initial Virologic Suppressors during Analytic Treatment Interruption in a Therapeutic HIV-1 gag Vaccine Trial
por: Li, Jonathan Z., et al.
Publicado: (2012)

Differential Vpu-Mediated CD4 and Tetherin Downregulation Functions among Major HIV-1 Group M Subtypes
por: Umviligihozo, Gisele, et al.
Publicado: (2020)

Inhibition of the TRIM24 bromodomain reactivates latent HIV-1
por: Horvath, Riley M., et al.
Publicado: (2023)

CDK8 inhibitors antagonize HIV-1 reactivation and promote provirus latency in T cells
por: Horvath, Riley M., et al.
Publicado: (2023)

Older Adults Mount Less Durable Humoral Responses to Two Doses of COVID-19 mRNA Vaccine but Strong Initial Responses to a Third Dose( )
por: Mwimanzi, Francis, et al.
Publicado: (2022)

Antigen Load and Viral Sequence Diversification Determine the Functional Profile of HIV-1–Specific CD8(+) T Cells
por: Streeck, Hendrik, et al.
Publicado: (2008)

Humoral immune responses to COVID-19 vaccination in people living with HIV receiving suppressive antiretroviral therapy
por: Brumme, Zabrina L., et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_p7m76lnsajbj4k9rpvpemqhlbi