Protocol for constructing large size human antibody heavy chain variable domain (V(H)) library and selection of SARS-CoV-2 neutralizing antibody domains

This protocol is a comprehensive guide to phage display-based selection of virus neutralizing V(H) antibody domains. It details three optimized parts including (1) construction of a large-sized (theoretically > 10(11)) naïve human antibody heavy chain domain library, (2) SARS-CoV-2 antigen expres...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Chuan, Sun, Zehua, Liu, Xianglei, Li, Wei, Dimitrov, Dimiter S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164376/
https://www.ncbi.nlm.nih.gov/pubmed/34095859
http://dx.doi.org/10.1016/j.xpro.2021.100617
Descripción
Sumario:This protocol is a comprehensive guide to phage display-based selection of virus neutralizing V(H) antibody domains. It details three optimized parts including (1) construction of a large-sized (theoretically > 10(11)) naïve human antibody heavy chain domain library, (2) SARS-CoV-2 antigen expression and stable cell line construction, and (3) library panning for selection of SARS-CoV-2-specific antibody domains. Using this protocol, we identified a high-affinity neutralizing human V(H) antibody domain, V(H) ab8, which exhibits high prophylactic and therapeutic efficacy. For complete details on the use and execution of this protocol, please refer to Li et al. (2020)

Ejemplares similares