Protocol for constructing large size human antibody heavy chain variable domain (V(H)) library and selection of SARS-CoV-2 neutralizing antibody domains

This protocol is a comprehensive guide to phage display-based selection of virus neutralizing V(H) antibody domains. It details three optimized parts including (1) construction of a large-sized (theoretically > 10(11)) naïve human antibody heavy chain domain library, (2) SARS-CoV-2 antigen expres...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Chuan, Sun, Zehua, Liu, Xianglei, Li, Wei, Dimitrov, Dimiter S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164376/
https://www.ncbi.nlm.nih.gov/pubmed/34095859
http://dx.doi.org/10.1016/j.xpro.2021.100617
_version_ 1783701102922825728
author Chen, Chuan
Sun, Zehua
Liu, Xianglei
Li, Wei
Dimitrov, Dimiter S.
author_facet Chen, Chuan
Sun, Zehua
Liu, Xianglei
Li, Wei
Dimitrov, Dimiter S.
author_sort Chen, Chuan
collection PubMed
description This protocol is a comprehensive guide to phage display-based selection of virus neutralizing V(H) antibody domains. It details three optimized parts including (1) construction of a large-sized (theoretically > 10(11)) naïve human antibody heavy chain domain library, (2) SARS-CoV-2 antigen expression and stable cell line construction, and (3) library panning for selection of SARS-CoV-2-specific antibody domains. Using this protocol, we identified a high-affinity neutralizing human V(H) antibody domain, V(H) ab8, which exhibits high prophylactic and therapeutic efficacy. For complete details on the use and execution of this protocol, please refer to Li et al. (2020)
format Online
Article
Text
id pubmed-8164376
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-81643762021-06-01 Protocol for constructing large size human antibody heavy chain variable domain (V(H)) library and selection of SARS-CoV-2 neutralizing antibody domains Chen, Chuan Sun, Zehua Liu, Xianglei Li, Wei Dimitrov, Dimiter S. STAR Protoc Protocol This protocol is a comprehensive guide to phage display-based selection of virus neutralizing V(H) antibody domains. It details three optimized parts including (1) construction of a large-sized (theoretically > 10(11)) naïve human antibody heavy chain domain library, (2) SARS-CoV-2 antigen expression and stable cell line construction, and (3) library panning for selection of SARS-CoV-2-specific antibody domains. Using this protocol, we identified a high-affinity neutralizing human V(H) antibody domain, V(H) ab8, which exhibits high prophylactic and therapeutic efficacy. For complete details on the use and execution of this protocol, please refer to Li et al. (2020) Elsevier 2021-05-29 /pmc/articles/PMC8164376/ /pubmed/34095859 http://dx.doi.org/10.1016/j.xpro.2021.100617 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Chen, Chuan
Sun, Zehua
Liu, Xianglei
Li, Wei
Dimitrov, Dimiter S.
Protocol for constructing large size human antibody heavy chain variable domain (V(H)) library and selection of SARS-CoV-2 neutralizing antibody domains
title Protocol for constructing large size human antibody heavy chain variable domain (V(H)) library and selection of SARS-CoV-2 neutralizing antibody domains
title_full Protocol for constructing large size human antibody heavy chain variable domain (V(H)) library and selection of SARS-CoV-2 neutralizing antibody domains
title_fullStr Protocol for constructing large size human antibody heavy chain variable domain (V(H)) library and selection of SARS-CoV-2 neutralizing antibody domains
title_full_unstemmed Protocol for constructing large size human antibody heavy chain variable domain (V(H)) library and selection of SARS-CoV-2 neutralizing antibody domains
title_short Protocol for constructing large size human antibody heavy chain variable domain (V(H)) library and selection of SARS-CoV-2 neutralizing antibody domains
title_sort protocol for constructing large size human antibody heavy chain variable domain (v(h)) library and selection of sars-cov-2 neutralizing antibody domains
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164376/
https://www.ncbi.nlm.nih.gov/pubmed/34095859
http://dx.doi.org/10.1016/j.xpro.2021.100617
work_keys_str_mv AT chenchuan protocolforconstructinglargesizehumanantibodyheavychainvariabledomainvhlibraryandselectionofsarscov2neutralizingantibodydomains
AT sunzehua protocolforconstructinglargesizehumanantibodyheavychainvariabledomainvhlibraryandselectionofsarscov2neutralizingantibodydomains
AT liuxianglei protocolforconstructinglargesizehumanantibodyheavychainvariabledomainvhlibraryandselectionofsarscov2neutralizingantibodydomains
AT liwei protocolforconstructinglargesizehumanantibodyheavychainvariabledomainvhlibraryandselectionofsarscov2neutralizingantibodydomains
AT dimitrovdimiters protocolforconstructinglargesizehumanantibodyheavychainvariabledomainvhlibraryandselectionofsarscov2neutralizingantibodydomains

Ejemplares similares