Cargando…

Role of Lymphotoxin-α Gene Polymorphism in Hepatitis C Virus-Related Chronic Liver Disorders

BACKGROUND: Tumor necrosis factor (TNF) family includes lymphotoxin-alpha (LTA) which is a pro-inflammatory cytokine which plays a role in hepatic fibrogenesis. LTA gene polymorphism plays a role in different inflammatory and immunomodulatory diseases. This polymorphism is also suggested to affect c...

Descripción completa

Detalles Bibliográficos
Autores principales: Galal, Ghada, Tammam, Hammam, Abdel Aal, Amal, Fahmy, Nahed, Sheneef, Abeer, Ahmed, Nagwa, Zaghloul, Amr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164674/
https://www.ncbi.nlm.nih.gov/pubmed/34079302
http://dx.doi.org/10.2147/IDR.S306879
_version_ 1783701165105479680
author Galal, Ghada
Tammam, Hammam
Abdel Aal, Amal
Fahmy, Nahed
Sheneef, Abeer
Ahmed, Nagwa
Zaghloul, Amr
author_facet Galal, Ghada
Tammam, Hammam
Abdel Aal, Amal
Fahmy, Nahed
Sheneef, Abeer
Ahmed, Nagwa
Zaghloul, Amr
author_sort Galal, Ghada
collection PubMed
description BACKGROUND: Tumor necrosis factor (TNF) family includes lymphotoxin-alpha (LTA) which is a pro-inflammatory cytokine which plays a role in hepatic fibrogenesis. LTA gene polymorphism plays a role in different inflammatory and immunomodulatory diseases. This polymorphism is also suggested to affect chronic hepatitis C (CHC) infection course. AIM: To study the contribution of LTA gene polymorphism in different chronic hepatitis C stages and hepatocellular carcinoma risk. PATIENTS AND METHODS: Our study included 108 chronic HCV patients grouped according to the disease stage. Group (A): CHC, group (B): liver cirrhosis (LC), group (C): LC with HCC, and group (D): healthy controls. Routine laboratory investigations, polymerase chain reaction (PCR) for quantification of HCV, abdominal ultrasonography, and Liver stiffness measurement (LSM) were done. Child–Turcotte–Pugh, Model for end-stage liver disease (MELD), and Fibrosis index based on 4 (FIB-4) scores were calculated. We used the PCR-restriction fragment length polymorphism technique for lymphotoxin-α genotyping. RESULTS: The A/G genotype was predominant in all groups. In HCC patients, G/G genotype was more frequent (31.8%) than in the LC group (19.4%), CHC group (17.8%), and controls (4.17%). A significant association was found between LTA genotypes and the child classes in HCC (P<0.01) but not in LC patients (P>0.05). HCC patients carrying A/G genotype had higher MELD scores than other genotypes. Multivariate binary logistic regression analysis confirmed that LTA G/G genotype and low platelet count were independent predictors for HCC development in patients with HCV-related LC. CONCLUSION: Detection of LTA G/G genotype in chronic HCV patients could help to recognize high-risk patients for disease progression and HCC development.
format Online
Article
Text
id pubmed-8164674
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Dove
record_format MEDLINE/PubMed
spelling pubmed-81646742021-06-01 Role of Lymphotoxin-α Gene Polymorphism in Hepatitis C Virus-Related Chronic Liver Disorders Galal, Ghada Tammam, Hammam Abdel Aal, Amal Fahmy, Nahed Sheneef, Abeer Ahmed, Nagwa Zaghloul, Amr Infect Drug Resist Original Research BACKGROUND: Tumor necrosis factor (TNF) family includes lymphotoxin-alpha (LTA) which is a pro-inflammatory cytokine which plays a role in hepatic fibrogenesis. LTA gene polymorphism plays a role in different inflammatory and immunomodulatory diseases. This polymorphism is also suggested to affect chronic hepatitis C (CHC) infection course. AIM: To study the contribution of LTA gene polymorphism in different chronic hepatitis C stages and hepatocellular carcinoma risk. PATIENTS AND METHODS: Our study included 108 chronic HCV patients grouped according to the disease stage. Group (A): CHC, group (B): liver cirrhosis (LC), group (C): LC with HCC, and group (D): healthy controls. Routine laboratory investigations, polymerase chain reaction (PCR) for quantification of HCV, abdominal ultrasonography, and Liver stiffness measurement (LSM) were done. Child–Turcotte–Pugh, Model for end-stage liver disease (MELD), and Fibrosis index based on 4 (FIB-4) scores were calculated. We used the PCR-restriction fragment length polymorphism technique for lymphotoxin-α genotyping. RESULTS: The A/G genotype was predominant in all groups. In HCC patients, G/G genotype was more frequent (31.8%) than in the LC group (19.4%), CHC group (17.8%), and controls (4.17%). A significant association was found between LTA genotypes and the child classes in HCC (P<0.01) but not in LC patients (P>0.05). HCC patients carrying A/G genotype had higher MELD scores than other genotypes. Multivariate binary logistic regression analysis confirmed that LTA G/G genotype and low platelet count were independent predictors for HCC development in patients with HCV-related LC. CONCLUSION: Detection of LTA G/G genotype in chronic HCV patients could help to recognize high-risk patients for disease progression and HCC development. Dove 2021-05-25 /pmc/articles/PMC8164674/ /pubmed/34079302 http://dx.doi.org/10.2147/IDR.S306879 Text en © 2021 Galal et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Galal, Ghada
Tammam, Hammam
Abdel Aal, Amal
Fahmy, Nahed
Sheneef, Abeer
Ahmed, Nagwa
Zaghloul, Amr
Role of Lymphotoxin-α Gene Polymorphism in Hepatitis C Virus-Related Chronic Liver Disorders
title Role of Lymphotoxin-α Gene Polymorphism in Hepatitis C Virus-Related Chronic Liver Disorders
title_full Role of Lymphotoxin-α Gene Polymorphism in Hepatitis C Virus-Related Chronic Liver Disorders
title_fullStr Role of Lymphotoxin-α Gene Polymorphism in Hepatitis C Virus-Related Chronic Liver Disorders
title_full_unstemmed Role of Lymphotoxin-α Gene Polymorphism in Hepatitis C Virus-Related Chronic Liver Disorders
title_short Role of Lymphotoxin-α Gene Polymorphism in Hepatitis C Virus-Related Chronic Liver Disorders
title_sort role of lymphotoxin-α gene polymorphism in hepatitis c virus-related chronic liver disorders
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164674/
https://www.ncbi.nlm.nih.gov/pubmed/34079302
http://dx.doi.org/10.2147/IDR.S306879
work_keys_str_mv AT galalghada roleoflymphotoxinagenepolymorphisminhepatitiscvirusrelatedchronicliverdisorders
AT tammamhammam roleoflymphotoxinagenepolymorphisminhepatitiscvirusrelatedchronicliverdisorders
AT abdelaalamal roleoflymphotoxinagenepolymorphisminhepatitiscvirusrelatedchronicliverdisorders
AT fahmynahed roleoflymphotoxinagenepolymorphisminhepatitiscvirusrelatedchronicliverdisorders
AT sheneefabeer roleoflymphotoxinagenepolymorphisminhepatitiscvirusrelatedchronicliverdisorders
AT ahmednagwa roleoflymphotoxinagenepolymorphisminhepatitiscvirusrelatedchronicliverdisorders
AT zaghloulamr roleoflymphotoxinagenepolymorphisminhepatitiscvirusrelatedchronicliverdisorders