Applicability of human-specific STR systems, GlobalFiler™ PCR Amplification Kit, Investigator 24plex QS Kit, and PowerPlex® Fusion 6C in chimpanzee (Pan troglodytes)

OBJECTIVES: Human identification systems based on STRs are widely used in human population genetics and forensic analysis. This study aimed to validate the cross-reactivity of three widely known human-specific STR identification systems i.e. GlobalFiler™ PCR Amplification Kit, Investigator 24plex QS...

Descripción completa

Detalles Bibliográficos
Autores principales: Singh, Abhishek, Sahajpal, Vivek, Thakur, Mukesh, Sharma, Lalit Kumar, Chandra, Kailash, Bhandari, Deepika, Sharma, Arun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164790/
https://www.ncbi.nlm.nih.gov/pubmed/34051836
http://dx.doi.org/10.1186/s13104-021-05632-6
_version_ 1783701190679199744
author Singh, Abhishek
Sahajpal, Vivek
Thakur, Mukesh
Sharma, Lalit Kumar
Chandra, Kailash
Bhandari, Deepika
Sharma, Arun
author_facet Singh, Abhishek
Sahajpal, Vivek
Thakur, Mukesh
Sharma, Lalit Kumar
Chandra, Kailash
Bhandari, Deepika
Sharma, Arun
author_sort Singh, Abhishek
collection PubMed
description OBJECTIVES: Human identification systems based on STRs are widely used in human population genetics and forensic analysis. This study aimed to validate the cross-reactivity of three widely known human-specific STR identification systems i.e. GlobalFiler™ PCR Amplification Kit, Investigator 24plex QS Kit, and PowerPlex® Fusion 6C in chimpanzee. RESULTS: The present study revealed the successful amplification of 18 loci using GlobalFiler™ PCR Amplification Kit, 18 loci using Investigator 24plex QS Kit, and 20 loci using PowerPlex® Fusion 6C system. The marker Amelogenin (AMEL) showed differential allele size between male and female revealing the gender identity of chimpanzees and thus validates their application concerning forensic examination, population estimation, and genetic analysis. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13104-021-05632-6.
format Online
Article
Text
id pubmed-8164790
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-81647902021-06-01 Applicability of human-specific STR systems, GlobalFiler™ PCR Amplification Kit, Investigator 24plex QS Kit, and PowerPlex® Fusion 6C in chimpanzee (Pan troglodytes) Singh, Abhishek Sahajpal, Vivek Thakur, Mukesh Sharma, Lalit Kumar Chandra, Kailash Bhandari, Deepika Sharma, Arun BMC Res Notes Research Note OBJECTIVES: Human identification systems based on STRs are widely used in human population genetics and forensic analysis. This study aimed to validate the cross-reactivity of three widely known human-specific STR identification systems i.e. GlobalFiler™ PCR Amplification Kit, Investigator 24plex QS Kit, and PowerPlex® Fusion 6C in chimpanzee. RESULTS: The present study revealed the successful amplification of 18 loci using GlobalFiler™ PCR Amplification Kit, 18 loci using Investigator 24plex QS Kit, and 20 loci using PowerPlex® Fusion 6C system. The marker Amelogenin (AMEL) showed differential allele size between male and female revealing the gender identity of chimpanzees and thus validates their application concerning forensic examination, population estimation, and genetic analysis. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13104-021-05632-6. BioMed Central 2021-05-29 /pmc/articles/PMC8164790/ /pubmed/34051836 http://dx.doi.org/10.1186/s13104-021-05632-6 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Note
Singh, Abhishek
Sahajpal, Vivek
Thakur, Mukesh
Sharma, Lalit Kumar
Chandra, Kailash
Bhandari, Deepika
Sharma, Arun
Applicability of human-specific STR systems, GlobalFiler™ PCR Amplification Kit, Investigator 24plex QS Kit, and PowerPlex® Fusion 6C in chimpanzee (Pan troglodytes)
title Applicability of human-specific STR systems, GlobalFiler™ PCR Amplification Kit, Investigator 24plex QS Kit, and PowerPlex® Fusion 6C in chimpanzee (Pan troglodytes)
title_full Applicability of human-specific STR systems, GlobalFiler™ PCR Amplification Kit, Investigator 24plex QS Kit, and PowerPlex® Fusion 6C in chimpanzee (Pan troglodytes)
title_fullStr Applicability of human-specific STR systems, GlobalFiler™ PCR Amplification Kit, Investigator 24plex QS Kit, and PowerPlex® Fusion 6C in chimpanzee (Pan troglodytes)
title_full_unstemmed Applicability of human-specific STR systems, GlobalFiler™ PCR Amplification Kit, Investigator 24plex QS Kit, and PowerPlex® Fusion 6C in chimpanzee (Pan troglodytes)
title_short Applicability of human-specific STR systems, GlobalFiler™ PCR Amplification Kit, Investigator 24plex QS Kit, and PowerPlex® Fusion 6C in chimpanzee (Pan troglodytes)
title_sort applicability of human-specific str systems, globalfiler™ pcr amplification kit, investigator 24plex qs kit, and powerplex® fusion 6c in chimpanzee (pan troglodytes)
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164790/
https://www.ncbi.nlm.nih.gov/pubmed/34051836
http://dx.doi.org/10.1186/s13104-021-05632-6
work_keys_str_mv AT singhabhishek applicabilityofhumanspecificstrsystemsglobalfilerpcramplificationkitinvestigator24plexqskitandpowerplexfusion6cinchimpanzeepantroglodytes
AT sahajpalvivek applicabilityofhumanspecificstrsystemsglobalfilerpcramplificationkitinvestigator24plexqskitandpowerplexfusion6cinchimpanzeepantroglodytes
AT thakurmukesh applicabilityofhumanspecificstrsystemsglobalfilerpcramplificationkitinvestigator24plexqskitandpowerplexfusion6cinchimpanzeepantroglodytes
AT sharmalalitkumar applicabilityofhumanspecificstrsystemsglobalfilerpcramplificationkitinvestigator24plexqskitandpowerplexfusion6cinchimpanzeepantroglodytes
AT chandrakailash applicabilityofhumanspecificstrsystemsglobalfilerpcramplificationkitinvestigator24plexqskitandpowerplexfusion6cinchimpanzeepantroglodytes
AT bhandarideepika applicabilityofhumanspecificstrsystemsglobalfilerpcramplificationkitinvestigator24plexqskitandpowerplexfusion6cinchimpanzeepantroglodytes
AT sharmaarun applicabilityofhumanspecificstrsystemsglobalfilerpcramplificationkitinvestigator24plexqskitandpowerplexfusion6cinchimpanzeepantroglodytes

Ejemplares similares