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Mvda is required for zebrafish early development

BACKGROUND: The MVD gene mutations are identified in porokeratosis, which is considered a skin-specific autoinflammatory keratinization disease. However, the biological function of MVD gene remains largely unknown. Therefore, we analyzed the function of mvda gene, orthologous to the human MVD gene,...

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Autores principales: Wong, Wenghong, Huang, Yike, Wu, Zhuanbin, Kong, Yu, Luan, Jing, Zhang, Qiaoan, Pan, Jiewen, Yan, Kexiang, Zhang, Zhenghua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164810/
https://www.ncbi.nlm.nih.gov/pubmed/34051853
http://dx.doi.org/10.1186/s40659-021-00341-7
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author Wong, Wenghong
Huang, Yike
Wu, Zhuanbin
Kong, Yu
Luan, Jing
Zhang, Qiaoan
Pan, Jiewen
Yan, Kexiang
Zhang, Zhenghua
author_facet Wong, Wenghong
Huang, Yike
Wu, Zhuanbin
Kong, Yu
Luan, Jing
Zhang, Qiaoan
Pan, Jiewen
Yan, Kexiang
Zhang, Zhenghua
author_sort Wong, Wenghong
collection PubMed
description BACKGROUND: The MVD gene mutations are identified in porokeratosis, which is considered a skin-specific autoinflammatory keratinization disease. However, the biological function of MVD gene remains largely unknown. Therefore, we analyzed the function of mvda gene, orthologous to the human MVD gene, in developing zebrafish. METHODS: Morpholino antisense oligonucleotide technique was used to generate mvda loss-of-function phenotypes. Knockdown of mvda was confirmed by RT-PCR and Sanger sequencing. Scanning and transmission electron microscopy were performed to analyze the morphology of the epidermis. Angiogenesis study was presented using the Tg(fli1a:EGFP)(y1) transgenic strain. In addition, acridine orange staining was used to examine the apoptotic cells in vivo. RESULTS: As expected, the mvda morphants showed abnormal morphology of the epidermis. Moreover, we observed ectopic sprouts in trunk angiogenesis and impaired formation of the caudal vein plexus in the mvda-deficient zebrafish. Besides, increased apoptosis was found throughout the tail, heart, and eyes in mvda zebrafish morphants. CONCLUSIONS: These findings indicated the essential role of mvda in the early development of zebrafish. This was the first in vivo knockdown study of the zebrafish mvda gene, which might offer insight into the biological function of the human MVD gene. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40659-021-00341-7.
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spelling pubmed-81648102021-06-01 Mvda is required for zebrafish early development Wong, Wenghong Huang, Yike Wu, Zhuanbin Kong, Yu Luan, Jing Zhang, Qiaoan Pan, Jiewen Yan, Kexiang Zhang, Zhenghua Biol Res Research Article BACKGROUND: The MVD gene mutations are identified in porokeratosis, which is considered a skin-specific autoinflammatory keratinization disease. However, the biological function of MVD gene remains largely unknown. Therefore, we analyzed the function of mvda gene, orthologous to the human MVD gene, in developing zebrafish. METHODS: Morpholino antisense oligonucleotide technique was used to generate mvda loss-of-function phenotypes. Knockdown of mvda was confirmed by RT-PCR and Sanger sequencing. Scanning and transmission electron microscopy were performed to analyze the morphology of the epidermis. Angiogenesis study was presented using the Tg(fli1a:EGFP)(y1) transgenic strain. In addition, acridine orange staining was used to examine the apoptotic cells in vivo. RESULTS: As expected, the mvda morphants showed abnormal morphology of the epidermis. Moreover, we observed ectopic sprouts in trunk angiogenesis and impaired formation of the caudal vein plexus in the mvda-deficient zebrafish. Besides, increased apoptosis was found throughout the tail, heart, and eyes in mvda zebrafish morphants. CONCLUSIONS: These findings indicated the essential role of mvda in the early development of zebrafish. This was the first in vivo knockdown study of the zebrafish mvda gene, which might offer insight into the biological function of the human MVD gene. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40659-021-00341-7. BioMed Central 2021-05-29 /pmc/articles/PMC8164810/ /pubmed/34051853 http://dx.doi.org/10.1186/s40659-021-00341-7 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Wong, Wenghong
Huang, Yike
Wu, Zhuanbin
Kong, Yu
Luan, Jing
Zhang, Qiaoan
Pan, Jiewen
Yan, Kexiang
Zhang, Zhenghua
Mvda is required for zebrafish early development
title Mvda is required for zebrafish early development
title_full Mvda is required for zebrafish early development
title_fullStr Mvda is required for zebrafish early development
title_full_unstemmed Mvda is required for zebrafish early development
title_short Mvda is required for zebrafish early development
title_sort mvda is required for zebrafish early development
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164810/
https://www.ncbi.nlm.nih.gov/pubmed/34051853
http://dx.doi.org/10.1186/s40659-021-00341-7
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