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The Influence of Silver Nanoparticles Against Toxic Effects of Philodryas olfersii Venom

PURPOSE: A silver nanoparticle obtained by reducing salts with solid dispersion of curcumin (130 nm, 0.081 mg mL(−1)) was used to counteract against the toxic – edematogenic, myotoxic, and neurotoxic – effects of Philodryas olfersii venom. METHODS: The edematogenic effect was evaluated by plasma ext...

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Autores principales: Proença-Assunção, Jaqueline de Cássia, Farias-de-França, Anna Paula, Tribuiani, Natalia, Cogo, Jose Carlos, Collaço, Rita de Cássia, Randazzo-Moura, Priscila, Consonni, Sílvio Roberto, Chaud, Marco Vinicius, dos Santos, Carolina Alves, Oshima-Franco, Yoko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164871/
https://www.ncbi.nlm.nih.gov/pubmed/34079248
http://dx.doi.org/10.2147/IJN.S293366
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author Proença-Assunção, Jaqueline de Cássia
Farias-de-França, Anna Paula
Tribuiani, Natalia
Cogo, Jose Carlos
Collaço, Rita de Cássia
Randazzo-Moura, Priscila
Consonni, Sílvio Roberto
Chaud, Marco Vinicius
dos Santos, Carolina Alves
Oshima-Franco, Yoko
author_facet Proença-Assunção, Jaqueline de Cássia
Farias-de-França, Anna Paula
Tribuiani, Natalia
Cogo, Jose Carlos
Collaço, Rita de Cássia
Randazzo-Moura, Priscila
Consonni, Sílvio Roberto
Chaud, Marco Vinicius
dos Santos, Carolina Alves
Oshima-Franco, Yoko
author_sort Proença-Assunção, Jaqueline de Cássia
collection PubMed
description PURPOSE: A silver nanoparticle obtained by reducing salts with solid dispersion of curcumin (130 nm, 0.081 mg mL(−1)) was used to counteract against the toxic – edematogenic, myotoxic, and neurotoxic – effects of Philodryas olfersii venom. METHODS: The edematogenic effect was evaluated by plasma extravasation in rat dorsal skin after injection of 50 µg per site of venom alone or preincubated with 1, 10, and 100 µL of AgNPs; the myotoxicity was evaluated by measuring the creatine kinase released into the organ-bath before the treatment and at the end of each experiment; and neurotoxicity was evaluated in chick biventer cervicis using the conventional myographic technique, face to the exogenous acetylcholine (ACh) and potassium chloride (KCl) added into the bath before the treatment and after each experiment. Preliminarily, a concentration-response curve of AgNPs was carried out to select the concentration to be used for neutralizing assays, which consists of neutralizing the venom-induced neuromuscular paralysis and edema by preincubating AgNPs with venom for 30 min. RESULTS: The P. olfersii venom-induced edema (n=6) and a complete neuromuscular blockade (n=4) that includes the total and unrecovered block of ACh and KCl contractures. AgNPs produced a concentration-dependent decrease the venom-induced edema (n=6) from 223.3% to 134.4% and to 100.5% after 10 and 100 µL AgNPs-preincubation, respectively. The preincubation of venom with AgNPs (1 µL; n=6) was able to maintain 46.5 ± 10.9% of neuromuscular response under indirect stimuli, 39.2 ± 9.7% of extrinsic nicotinic receptors functioning in absence of electrical stimulus and 28.3 ± 8.1% of responsiveness to potassium on the sarcolemmal membrane. The CK release was not affected by any experimental protocol which was like control. CONCLUSION: AgNPs interact with constituents of P. olfersii venom responsible for the edema-forming activity and neuromuscular blockade, but not on the sarcolemma membrane-acting constituents. The protective effect of the studied AgNPs on avian preparation points out to molecular targets as intrinsic and extrinsic nicotinic receptors.
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spelling pubmed-81648712021-06-01 The Influence of Silver Nanoparticles Against Toxic Effects of Philodryas olfersii Venom Proença-Assunção, Jaqueline de Cássia Farias-de-França, Anna Paula Tribuiani, Natalia Cogo, Jose Carlos Collaço, Rita de Cássia Randazzo-Moura, Priscila Consonni, Sílvio Roberto Chaud, Marco Vinicius dos Santos, Carolina Alves Oshima-Franco, Yoko Int J Nanomedicine Original Research PURPOSE: A silver nanoparticle obtained by reducing salts with solid dispersion of curcumin (130 nm, 0.081 mg mL(−1)) was used to counteract against the toxic – edematogenic, myotoxic, and neurotoxic – effects of Philodryas olfersii venom. METHODS: The edematogenic effect was evaluated by plasma extravasation in rat dorsal skin after injection of 50 µg per site of venom alone or preincubated with 1, 10, and 100 µL of AgNPs; the myotoxicity was evaluated by measuring the creatine kinase released into the organ-bath before the treatment and at the end of each experiment; and neurotoxicity was evaluated in chick biventer cervicis using the conventional myographic technique, face to the exogenous acetylcholine (ACh) and potassium chloride (KCl) added into the bath before the treatment and after each experiment. Preliminarily, a concentration-response curve of AgNPs was carried out to select the concentration to be used for neutralizing assays, which consists of neutralizing the venom-induced neuromuscular paralysis and edema by preincubating AgNPs with venom for 30 min. RESULTS: The P. olfersii venom-induced edema (n=6) and a complete neuromuscular blockade (n=4) that includes the total and unrecovered block of ACh and KCl contractures. AgNPs produced a concentration-dependent decrease the venom-induced edema (n=6) from 223.3% to 134.4% and to 100.5% after 10 and 100 µL AgNPs-preincubation, respectively. The preincubation of venom with AgNPs (1 µL; n=6) was able to maintain 46.5 ± 10.9% of neuromuscular response under indirect stimuli, 39.2 ± 9.7% of extrinsic nicotinic receptors functioning in absence of electrical stimulus and 28.3 ± 8.1% of responsiveness to potassium on the sarcolemmal membrane. The CK release was not affected by any experimental protocol which was like control. CONCLUSION: AgNPs interact with constituents of P. olfersii venom responsible for the edema-forming activity and neuromuscular blockade, but not on the sarcolemma membrane-acting constituents. The protective effect of the studied AgNPs on avian preparation points out to molecular targets as intrinsic and extrinsic nicotinic receptors. Dove 2021-05-25 /pmc/articles/PMC8164871/ /pubmed/34079248 http://dx.doi.org/10.2147/IJN.S293366 Text en © 2021 Proença-Assunção et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Proença-Assunção, Jaqueline de Cássia
Farias-de-França, Anna Paula
Tribuiani, Natalia
Cogo, Jose Carlos
Collaço, Rita de Cássia
Randazzo-Moura, Priscila
Consonni, Sílvio Roberto
Chaud, Marco Vinicius
dos Santos, Carolina Alves
Oshima-Franco, Yoko
The Influence of Silver Nanoparticles Against Toxic Effects of Philodryas olfersii Venom
title The Influence of Silver Nanoparticles Against Toxic Effects of Philodryas olfersii Venom
title_full The Influence of Silver Nanoparticles Against Toxic Effects of Philodryas olfersii Venom
title_fullStr The Influence of Silver Nanoparticles Against Toxic Effects of Philodryas olfersii Venom
title_full_unstemmed The Influence of Silver Nanoparticles Against Toxic Effects of Philodryas olfersii Venom
title_short The Influence of Silver Nanoparticles Against Toxic Effects of Philodryas olfersii Venom
title_sort influence of silver nanoparticles against toxic effects of philodryas olfersii venom
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164871/
https://www.ncbi.nlm.nih.gov/pubmed/34079248
http://dx.doi.org/10.2147/IJN.S293366
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