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Viral RNA in City Wastewater as a Key Indicator of COVID-19 Recrudescence and Containment Measures Effectiveness

In recent years, and more specifically at the beginning of the COVID-19 crisis, wastewater surveillance has been proposed as a tool to monitor the epidemiology of human viral infections. In the present work, from July to December 2020, the number of copies of SARS-CoV-2 RNA in Marseille’s wastewater...

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Autores principales: Wurtz, Nathalie, Lacoste, Alexandre, Jardot, Priscilla, Delache, Alain, Fontaine, Xavier, Verlande, Maxime, Annessi, Alexandre, Giraud-Gatineau, Audrey, Chaudet, Hervé, Fournier, Pierre-Edouard, Augier, Patrick, La Scola, Bernard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8165276/
https://www.ncbi.nlm.nih.gov/pubmed/34079532
http://dx.doi.org/10.3389/fmicb.2021.664477
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author Wurtz, Nathalie
Lacoste, Alexandre
Jardot, Priscilla
Delache, Alain
Fontaine, Xavier
Verlande, Maxime
Annessi, Alexandre
Giraud-Gatineau, Audrey
Chaudet, Hervé
Fournier, Pierre-Edouard
Augier, Patrick
La Scola, Bernard
author_facet Wurtz, Nathalie
Lacoste, Alexandre
Jardot, Priscilla
Delache, Alain
Fontaine, Xavier
Verlande, Maxime
Annessi, Alexandre
Giraud-Gatineau, Audrey
Chaudet, Hervé
Fournier, Pierre-Edouard
Augier, Patrick
La Scola, Bernard
author_sort Wurtz, Nathalie
collection PubMed
description In recent years, and more specifically at the beginning of the COVID-19 crisis, wastewater surveillance has been proposed as a tool to monitor the epidemiology of human viral infections. In the present work, from July to December 2020, the number of copies of SARS-CoV-2 RNA in Marseille’s wastewater was correlated with the number of new positive cases diagnosed in our Institute of Infectious Disease, which tested about 20% of the city’s population. Number of positive cases and number of copies of SARS-CoV-2 RNA in wastewater were significantly correlated (p = 0.013). During the great epidemic peak, from October to December 2020, the curves of virus in the sewers and the curves of positive diagnoses were perfectly superposed. During the summer period, the superposition of curves was less evident as subject to many confounding factors that were discussed. We also tried to correlate the effect of viral circulation in wastewater with containment measures, probably the most unbiased correlation on their potential inflection effect of epidemic curves. Not only is this correlation not obvious, but it also clearly appears that the drop in cases as well as the drop in the viral load in the sewers occur before the containment measures. In fact, this suggests that there are factors that initiate the end of the epidemic peak independently of the containment measure. These factors will therefore need to be explored more deeply in the future.
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spelling pubmed-81652762021-06-01 Viral RNA in City Wastewater as a Key Indicator of COVID-19 Recrudescence and Containment Measures Effectiveness Wurtz, Nathalie Lacoste, Alexandre Jardot, Priscilla Delache, Alain Fontaine, Xavier Verlande, Maxime Annessi, Alexandre Giraud-Gatineau, Audrey Chaudet, Hervé Fournier, Pierre-Edouard Augier, Patrick La Scola, Bernard Front Microbiol Microbiology In recent years, and more specifically at the beginning of the COVID-19 crisis, wastewater surveillance has been proposed as a tool to monitor the epidemiology of human viral infections. In the present work, from July to December 2020, the number of copies of SARS-CoV-2 RNA in Marseille’s wastewater was correlated with the number of new positive cases diagnosed in our Institute of Infectious Disease, which tested about 20% of the city’s population. Number of positive cases and number of copies of SARS-CoV-2 RNA in wastewater were significantly correlated (p = 0.013). During the great epidemic peak, from October to December 2020, the curves of virus in the sewers and the curves of positive diagnoses were perfectly superposed. During the summer period, the superposition of curves was less evident as subject to many confounding factors that were discussed. We also tried to correlate the effect of viral circulation in wastewater with containment measures, probably the most unbiased correlation on their potential inflection effect of epidemic curves. Not only is this correlation not obvious, but it also clearly appears that the drop in cases as well as the drop in the viral load in the sewers occur before the containment measures. In fact, this suggests that there are factors that initiate the end of the epidemic peak independently of the containment measure. These factors will therefore need to be explored more deeply in the future. Frontiers Media S.A. 2021-05-17 /pmc/articles/PMC8165276/ /pubmed/34079532 http://dx.doi.org/10.3389/fmicb.2021.664477 Text en Copyright © 2021 Wurtz, Lacoste, Jardot, Delache, Fontaine, Verlande, Annessi, Giraud-Gatineau, Chaudet, Fournier, Augier and La Scola. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Wurtz, Nathalie
Lacoste, Alexandre
Jardot, Priscilla
Delache, Alain
Fontaine, Xavier
Verlande, Maxime
Annessi, Alexandre
Giraud-Gatineau, Audrey
Chaudet, Hervé
Fournier, Pierre-Edouard
Augier, Patrick
La Scola, Bernard
Viral RNA in City Wastewater as a Key Indicator of COVID-19 Recrudescence and Containment Measures Effectiveness
title Viral RNA in City Wastewater as a Key Indicator of COVID-19 Recrudescence and Containment Measures Effectiveness
title_full Viral RNA in City Wastewater as a Key Indicator of COVID-19 Recrudescence and Containment Measures Effectiveness
title_fullStr Viral RNA in City Wastewater as a Key Indicator of COVID-19 Recrudescence and Containment Measures Effectiveness
title_full_unstemmed Viral RNA in City Wastewater as a Key Indicator of COVID-19 Recrudescence and Containment Measures Effectiveness
title_short Viral RNA in City Wastewater as a Key Indicator of COVID-19 Recrudescence and Containment Measures Effectiveness
title_sort viral rna in city wastewater as a key indicator of covid-19 recrudescence and containment measures effectiveness
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8165276/
https://www.ncbi.nlm.nih.gov/pubmed/34079532
http://dx.doi.org/10.3389/fmicb.2021.664477
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