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He-Ne Laser Seed Treatment Improves the Nutraceutical Metabolic Pool of Sunflowers and Provides Better Tolerance Against Water Deficit
Water-scarce areas are continually increasing worldwide. This factor reduces the quantity and quality of crops produced in affected areas. Physical seed treatments are considered economical and ecofriendly solutions for such problems. It was hypothesized that a moderately drought-tolerant crop grown...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8165324/ https://www.ncbi.nlm.nih.gov/pubmed/34079562 http://dx.doi.org/10.3389/fpls.2021.579429 |
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author | Mahmood, Saqib Afzal, Beenish Perveen, Shagufta Wahid, Abdul Azeem, Muhammad Iqbal, Naeem |
author_facet | Mahmood, Saqib Afzal, Beenish Perveen, Shagufta Wahid, Abdul Azeem, Muhammad Iqbal, Naeem |
author_sort | Mahmood, Saqib |
collection | PubMed |
description | Water-scarce areas are continually increasing worldwide. This factor reduces the quantity and quality of crops produced in affected areas. Physical seed treatments are considered economical and ecofriendly solutions for such problems. It was hypothesized that a moderately drought-tolerant crop grown from seeds treated with a He-Ne laser utilizes water-limited conditions better than plants grown from untreated seeds. A field study was conducted, growing a moderately drought tolerant crop (sunflower) with supportive seed treatment (He-Ne laser treatment at 300 mJ) for 0, 1, 2, and 3 min. Thirty-day-old plants were subjected to two irrigation conditions: 100% (normal) and 50% (water stress). Harvesting was done at flowering (60-day-old plants) at full maturity. The sunflowers maintained growth and yield under water limitation with a reduced achene number. At 50%, irrigation, there was a reduction in chlorophyll a, a+b and a/b; catalase activity; soluble sugars; and anthocyanin, alongside elevated proline. The improved chlorophyll a, a+b and a/b; metabolisable energy; nutritional value; and yield in the plants grown from He-Ne-laser-treated seeds support our hypothesis. Seeds with 2-min exposure to a He-Ne laser performed best regarding leaf area; leaf number; leaf biomass; chlorophyll a, a+b and a/b; per cent oil yield; 50-achene weight; achene weight per plant; carotenoid content; and total soluble phenolic compound content. Thereafter, the leaves from the best performing level of treatment (2 min) were subjected to high-performance-liquid-chromatography-based phenolic profiling and gas-chromatography-based fatty acid profiling of the oil yield. The He-Ne laser treatment led to the accumulation of nutraceutical phenolic compounds and improved the unsaturated-to-saturated fatty acid ratio of the oil. In conclusion, 2-min He-Ne laser seed treatment could be the best strategy to improve the yield and nutritional value of sunflowers grown in water-limited areas. |
format | Online Article Text |
id | pubmed-8165324 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-81653242021-06-01 He-Ne Laser Seed Treatment Improves the Nutraceutical Metabolic Pool of Sunflowers and Provides Better Tolerance Against Water Deficit Mahmood, Saqib Afzal, Beenish Perveen, Shagufta Wahid, Abdul Azeem, Muhammad Iqbal, Naeem Front Plant Sci Plant Science Water-scarce areas are continually increasing worldwide. This factor reduces the quantity and quality of crops produced in affected areas. Physical seed treatments are considered economical and ecofriendly solutions for such problems. It was hypothesized that a moderately drought-tolerant crop grown from seeds treated with a He-Ne laser utilizes water-limited conditions better than plants grown from untreated seeds. A field study was conducted, growing a moderately drought tolerant crop (sunflower) with supportive seed treatment (He-Ne laser treatment at 300 mJ) for 0, 1, 2, and 3 min. Thirty-day-old plants were subjected to two irrigation conditions: 100% (normal) and 50% (water stress). Harvesting was done at flowering (60-day-old plants) at full maturity. The sunflowers maintained growth and yield under water limitation with a reduced achene number. At 50%, irrigation, there was a reduction in chlorophyll a, a+b and a/b; catalase activity; soluble sugars; and anthocyanin, alongside elevated proline. The improved chlorophyll a, a+b and a/b; metabolisable energy; nutritional value; and yield in the plants grown from He-Ne-laser-treated seeds support our hypothesis. Seeds with 2-min exposure to a He-Ne laser performed best regarding leaf area; leaf number; leaf biomass; chlorophyll a, a+b and a/b; per cent oil yield; 50-achene weight; achene weight per plant; carotenoid content; and total soluble phenolic compound content. Thereafter, the leaves from the best performing level of treatment (2 min) were subjected to high-performance-liquid-chromatography-based phenolic profiling and gas-chromatography-based fatty acid profiling of the oil yield. The He-Ne laser treatment led to the accumulation of nutraceutical phenolic compounds and improved the unsaturated-to-saturated fatty acid ratio of the oil. In conclusion, 2-min He-Ne laser seed treatment could be the best strategy to improve the yield and nutritional value of sunflowers grown in water-limited areas. Frontiers Media S.A. 2021-05-17 /pmc/articles/PMC8165324/ /pubmed/34079562 http://dx.doi.org/10.3389/fpls.2021.579429 Text en Copyright © 2021 Mahmood, Afzal, Perveen, Wahid, Azeem and Iqbal. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Mahmood, Saqib Afzal, Beenish Perveen, Shagufta Wahid, Abdul Azeem, Muhammad Iqbal, Naeem He-Ne Laser Seed Treatment Improves the Nutraceutical Metabolic Pool of Sunflowers and Provides Better Tolerance Against Water Deficit |
title | He-Ne Laser Seed Treatment Improves the Nutraceutical Metabolic Pool of Sunflowers and Provides Better Tolerance Against Water Deficit |
title_full | He-Ne Laser Seed Treatment Improves the Nutraceutical Metabolic Pool of Sunflowers and Provides Better Tolerance Against Water Deficit |
title_fullStr | He-Ne Laser Seed Treatment Improves the Nutraceutical Metabolic Pool of Sunflowers and Provides Better Tolerance Against Water Deficit |
title_full_unstemmed | He-Ne Laser Seed Treatment Improves the Nutraceutical Metabolic Pool of Sunflowers and Provides Better Tolerance Against Water Deficit |
title_short | He-Ne Laser Seed Treatment Improves the Nutraceutical Metabolic Pool of Sunflowers and Provides Better Tolerance Against Water Deficit |
title_sort | he-ne laser seed treatment improves the nutraceutical metabolic pool of sunflowers and provides better tolerance against water deficit |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8165324/ https://www.ncbi.nlm.nih.gov/pubmed/34079562 http://dx.doi.org/10.3389/fpls.2021.579429 |
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