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Brain HIV-1 latently-infected reservoirs targeted by the suicide gene strategy
Reducing the pool of HIV-1 reservoirs in patients is a must to achieve functional cure. The most prominent HIV-1 cell reservoirs are resting CD4 + T cells and brain derived microglial cells. Infected microglial cells are believed to be the source of peripheral tissues reseedings and the emergence of...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8166011/ https://www.ncbi.nlm.nih.gov/pubmed/34059075 http://dx.doi.org/10.1186/s12985-021-01584-2 |
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author | Saeb, Sepideh Ravanshad, Mehrdad Pourkarim, Mahmoud Reza Daouad, Fadoua Baesi, Kazem Rohr, Olivier Wallet, Clémentine Schwartz, Christian |
author_facet | Saeb, Sepideh Ravanshad, Mehrdad Pourkarim, Mahmoud Reza Daouad, Fadoua Baesi, Kazem Rohr, Olivier Wallet, Clémentine Schwartz, Christian |
author_sort | Saeb, Sepideh |
collection | PubMed |
description | Reducing the pool of HIV-1 reservoirs in patients is a must to achieve functional cure. The most prominent HIV-1 cell reservoirs are resting CD4 + T cells and brain derived microglial cells. Infected microglial cells are believed to be the source of peripheral tissues reseedings and the emergence of drug resistance. Clearing infected cells from the brain is therefore crucial. However, many characteristics of microglial cells and the central nervous system make extremely difficult their eradication from brain reservoirs. Current methods, such as the “shock and kill”, the “block and lock” and gene editing strategies cannot override these difficulties. Therefore, new strategies have to be designed when considering the elimination of brain reservoirs. We set up an original gene suicide strategy using latently infected microglial cells as model cells. In this paper we provide proof of concept of this strategy. |
format | Online Article Text |
id | pubmed-8166011 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-81660112021-06-02 Brain HIV-1 latently-infected reservoirs targeted by the suicide gene strategy Saeb, Sepideh Ravanshad, Mehrdad Pourkarim, Mahmoud Reza Daouad, Fadoua Baesi, Kazem Rohr, Olivier Wallet, Clémentine Schwartz, Christian Virol J Short Report Reducing the pool of HIV-1 reservoirs in patients is a must to achieve functional cure. The most prominent HIV-1 cell reservoirs are resting CD4 + T cells and brain derived microglial cells. Infected microglial cells are believed to be the source of peripheral tissues reseedings and the emergence of drug resistance. Clearing infected cells from the brain is therefore crucial. However, many characteristics of microglial cells and the central nervous system make extremely difficult their eradication from brain reservoirs. Current methods, such as the “shock and kill”, the “block and lock” and gene editing strategies cannot override these difficulties. Therefore, new strategies have to be designed when considering the elimination of brain reservoirs. We set up an original gene suicide strategy using latently infected microglial cells as model cells. In this paper we provide proof of concept of this strategy. BioMed Central 2021-05-31 /pmc/articles/PMC8166011/ /pubmed/34059075 http://dx.doi.org/10.1186/s12985-021-01584-2 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Short Report Saeb, Sepideh Ravanshad, Mehrdad Pourkarim, Mahmoud Reza Daouad, Fadoua Baesi, Kazem Rohr, Olivier Wallet, Clémentine Schwartz, Christian Brain HIV-1 latently-infected reservoirs targeted by the suicide gene strategy |
title | Brain HIV-1 latently-infected reservoirs targeted by the suicide gene strategy |
title_full | Brain HIV-1 latently-infected reservoirs targeted by the suicide gene strategy |
title_fullStr | Brain HIV-1 latently-infected reservoirs targeted by the suicide gene strategy |
title_full_unstemmed | Brain HIV-1 latently-infected reservoirs targeted by the suicide gene strategy |
title_short | Brain HIV-1 latently-infected reservoirs targeted by the suicide gene strategy |
title_sort | brain hiv-1 latently-infected reservoirs targeted by the suicide gene strategy |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8166011/ https://www.ncbi.nlm.nih.gov/pubmed/34059075 http://dx.doi.org/10.1186/s12985-021-01584-2 |
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