Cargando…
Co-transfection with BMP2 and FGF2 via chitosan nanoparticles potentiates osteogenesis in human adipose-derived stromal cells in vitro
OBJECTIVE: To investigate if co-transfection of human bone morphogenetic protein 2 (BMP-2, BMP2) and human fibroblast growth factor 2 (FGF2, FGF2) via chitosan nanoparticles promotes osteogenesis in human adipose tissue-derived stem cells (ADSCs) in vitro. MATERIALS AND METHODS: Recombinant BMP2 and...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8166400/ https://www.ncbi.nlm.nih.gov/pubmed/33769121 http://dx.doi.org/10.1177/0300060521997679 |
_version_ | 1783701499038138368 |
---|---|
author | Hu, Ying Zhao, Qing-Wei Wang, Zheng-Cai Fang, Qing-Qing Zhu, He Hong, Dong-Sheng Liang, Xing-Guang Lou, Dong Tan, Wei-Qiang |
author_facet | Hu, Ying Zhao, Qing-Wei Wang, Zheng-Cai Fang, Qing-Qing Zhu, He Hong, Dong-Sheng Liang, Xing-Guang Lou, Dong Tan, Wei-Qiang |
author_sort | Hu, Ying |
collection | PubMed |
description | OBJECTIVE: To investigate if co-transfection of human bone morphogenetic protein 2 (BMP-2, BMP2) and human fibroblast growth factor 2 (FGF2, FGF2) via chitosan nanoparticles promotes osteogenesis in human adipose tissue-derived stem cells (ADSCs) in vitro. MATERIALS AND METHODS: Recombinant BMP2 and/or FGF2 expression vectors were constructed and packaged into chitosan nanoparticles. The chitosan nanoparticles were characterized by atomic force microscopy. Gene and protein expression levels of BMP-2 and FGF2 in ADSCs in vitro were evaluated by real-time polymerase chain reaction (PCR), western blot, and enzyme-linked immunosorbent assay. Osteocalcin (OCN) and bone sialoprotein (BSP) gene expression were also evaluated by real-time PCR to assess osteogenesis. RESULTS: The prepared chitosan nanoparticles were spherical with a relatively homogenous size distribution. The BMP2 and FGF2 vectors were successfully transfected into ADSCs. BMP-2 and FGF2 mRNA and protein levels were significantly up-regulated in the co-transfection group compared with the control group. OCN and BSP mRNA levels were also significantly increased in the co-transfection group compared with cells transfected with BMP2 or FGF2 alone, suggesting that co-transfection significantly enhanced osteogenesis. CONCLUSIONS: Co-transfection of human ADSCs with BMP2/FGF2 via chitosan nanoparticles efficiently promotes the osteogenic properties of ADSCs in vitro. |
format | Online Article Text |
id | pubmed-8166400 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-81664002021-06-07 Co-transfection with BMP2 and FGF2 via chitosan nanoparticles potentiates osteogenesis in human adipose-derived stromal cells in vitro Hu, Ying Zhao, Qing-Wei Wang, Zheng-Cai Fang, Qing-Qing Zhu, He Hong, Dong-Sheng Liang, Xing-Guang Lou, Dong Tan, Wei-Qiang J Int Med Res Pre-Clinical Research Report OBJECTIVE: To investigate if co-transfection of human bone morphogenetic protein 2 (BMP-2, BMP2) and human fibroblast growth factor 2 (FGF2, FGF2) via chitosan nanoparticles promotes osteogenesis in human adipose tissue-derived stem cells (ADSCs) in vitro. MATERIALS AND METHODS: Recombinant BMP2 and/or FGF2 expression vectors were constructed and packaged into chitosan nanoparticles. The chitosan nanoparticles were characterized by atomic force microscopy. Gene and protein expression levels of BMP-2 and FGF2 in ADSCs in vitro were evaluated by real-time polymerase chain reaction (PCR), western blot, and enzyme-linked immunosorbent assay. Osteocalcin (OCN) and bone sialoprotein (BSP) gene expression were also evaluated by real-time PCR to assess osteogenesis. RESULTS: The prepared chitosan nanoparticles were spherical with a relatively homogenous size distribution. The BMP2 and FGF2 vectors were successfully transfected into ADSCs. BMP-2 and FGF2 mRNA and protein levels were significantly up-regulated in the co-transfection group compared with the control group. OCN and BSP mRNA levels were also significantly increased in the co-transfection group compared with cells transfected with BMP2 or FGF2 alone, suggesting that co-transfection significantly enhanced osteogenesis. CONCLUSIONS: Co-transfection of human ADSCs with BMP2/FGF2 via chitosan nanoparticles efficiently promotes the osteogenic properties of ADSCs in vitro. SAGE Publications 2021-03-26 /pmc/articles/PMC8166400/ /pubmed/33769121 http://dx.doi.org/10.1177/0300060521997679 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Pre-Clinical Research Report Hu, Ying Zhao, Qing-Wei Wang, Zheng-Cai Fang, Qing-Qing Zhu, He Hong, Dong-Sheng Liang, Xing-Guang Lou, Dong Tan, Wei-Qiang Co-transfection with BMP2 and FGF2 via chitosan nanoparticles potentiates osteogenesis in human adipose-derived stromal cells in vitro |
title | Co-transfection with BMP2 and FGF2 via chitosan nanoparticles potentiates osteogenesis in human adipose-derived stromal cells in vitro |
title_full | Co-transfection with BMP2 and FGF2 via chitosan nanoparticles potentiates osteogenesis in human adipose-derived stromal cells in vitro |
title_fullStr | Co-transfection with BMP2 and FGF2 via chitosan nanoparticles potentiates osteogenesis in human adipose-derived stromal cells in vitro |
title_full_unstemmed | Co-transfection with BMP2 and FGF2 via chitosan nanoparticles potentiates osteogenesis in human adipose-derived stromal cells in vitro |
title_short | Co-transfection with BMP2 and FGF2 via chitosan nanoparticles potentiates osteogenesis in human adipose-derived stromal cells in vitro |
title_sort | co-transfection with bmp2 and fgf2 via chitosan nanoparticles potentiates osteogenesis in human adipose-derived stromal cells in vitro |
topic | Pre-Clinical Research Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8166400/ https://www.ncbi.nlm.nih.gov/pubmed/33769121 http://dx.doi.org/10.1177/0300060521997679 |
work_keys_str_mv | AT huying cotransfectionwithbmp2andfgf2viachitosannanoparticlespotentiatesosteogenesisinhumanadiposederivedstromalcellsinvitro AT zhaoqingwei cotransfectionwithbmp2andfgf2viachitosannanoparticlespotentiatesosteogenesisinhumanadiposederivedstromalcellsinvitro AT wangzhengcai cotransfectionwithbmp2andfgf2viachitosannanoparticlespotentiatesosteogenesisinhumanadiposederivedstromalcellsinvitro AT fangqingqing cotransfectionwithbmp2andfgf2viachitosannanoparticlespotentiatesosteogenesisinhumanadiposederivedstromalcellsinvitro AT zhuhe cotransfectionwithbmp2andfgf2viachitosannanoparticlespotentiatesosteogenesisinhumanadiposederivedstromalcellsinvitro AT hongdongsheng cotransfectionwithbmp2andfgf2viachitosannanoparticlespotentiatesosteogenesisinhumanadiposederivedstromalcellsinvitro AT liangxingguang cotransfectionwithbmp2andfgf2viachitosannanoparticlespotentiatesosteogenesisinhumanadiposederivedstromalcellsinvitro AT loudong cotransfectionwithbmp2andfgf2viachitosannanoparticlespotentiatesosteogenesisinhumanadiposederivedstromalcellsinvitro AT tanweiqiang cotransfectionwithbmp2andfgf2viachitosannanoparticlespotentiatesosteogenesisinhumanadiposederivedstromalcellsinvitro |