Nasopharyngeal Microbiota in SARS-CoV-2 Positive and Negative Patients

We investigated nasopharyngeal microbial community structure in COVID-19-positive and -negative patients. High-throughput 16S ribosomal RNA gene amplicon sequencing revealed significant microbial community structure differences between COVID-19-positive and -negative patients. This proof-of-concept...

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Autores principales: Engen, Phillip A., Naqib, Ankur, Jennings, Cheryl, Green, Stefan J., Landay, Alan, Keshavarzian, Ali, Voigt, Robin M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8166531/
https://www.ncbi.nlm.nih.gov/pubmed/34058978
http://dx.doi.org/10.1186/s12575-021-00148-6
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author Engen, Phillip A.
Naqib, Ankur
Jennings, Cheryl
Green, Stefan J.
Landay, Alan
Keshavarzian, Ali
Voigt, Robin M.
author_facet Engen, Phillip A.
Naqib, Ankur
Jennings, Cheryl
Green, Stefan J.
Landay, Alan
Keshavarzian, Ali
Voigt, Robin M.
author_sort Engen, Phillip A.
collection PubMed
description We investigated nasopharyngeal microbial community structure in COVID-19-positive and -negative patients. High-throughput 16S ribosomal RNA gene amplicon sequencing revealed significant microbial community structure differences between COVID-19-positive and -negative patients. This proof-of-concept study demonstrates that: (1) nasopharyngeal microbiome communities can be assessed using collection samples already collected for SARS-CoV-2 testing (viral transport media) and (2) SARS-CoV-2 infection is associated with altered dysbiotic microbial profiles which could be a biomarker for disease progression and prognosis in SARS-CoV-2. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12575-021-00148-6.
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spelling pubmed-81665312021-06-01 Nasopharyngeal Microbiota in SARS-CoV-2 Positive and Negative Patients Engen, Phillip A. Naqib, Ankur Jennings, Cheryl Green, Stefan J. Landay, Alan Keshavarzian, Ali Voigt, Robin M. Biol Proced Online Short Report We investigated nasopharyngeal microbial community structure in COVID-19-positive and -negative patients. High-throughput 16S ribosomal RNA gene amplicon sequencing revealed significant microbial community structure differences between COVID-19-positive and -negative patients. This proof-of-concept study demonstrates that: (1) nasopharyngeal microbiome communities can be assessed using collection samples already collected for SARS-CoV-2 testing (viral transport media) and (2) SARS-CoV-2 infection is associated with altered dysbiotic microbial profiles which could be a biomarker for disease progression and prognosis in SARS-CoV-2. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12575-021-00148-6. BioMed Central 2021-06-01 /pmc/articles/PMC8166531/ /pubmed/34058978 http://dx.doi.org/10.1186/s12575-021-00148-6 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Short Report
Engen, Phillip A.
Naqib, Ankur
Jennings, Cheryl
Green, Stefan J.
Landay, Alan
Keshavarzian, Ali
Voigt, Robin M.
Nasopharyngeal Microbiota in SARS-CoV-2 Positive and Negative Patients
title Nasopharyngeal Microbiota in SARS-CoV-2 Positive and Negative Patients
title_full Nasopharyngeal Microbiota in SARS-CoV-2 Positive and Negative Patients
title_fullStr Nasopharyngeal Microbiota in SARS-CoV-2 Positive and Negative Patients
title_full_unstemmed Nasopharyngeal Microbiota in SARS-CoV-2 Positive and Negative Patients
title_short Nasopharyngeal Microbiota in SARS-CoV-2 Positive and Negative Patients
title_sort nasopharyngeal microbiota in sars-cov-2 positive and negative patients
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8166531/
https://www.ncbi.nlm.nih.gov/pubmed/34058978
http://dx.doi.org/10.1186/s12575-021-00148-6
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