Cargando…

Comprehensive Proteomic Analysis of Lysine Acetylation in Nicotiana benthamiana After Sensing CWMV Infection

Protein lysine acetylation (Kac) is an important post-translational modification mechanism in eukaryotes that is involved in cellular regulation. To investigate the role of Kac in virus-infected plants, we characterized the lysine acetylome of Nicotiana benthamiana plants with or without a Chinese w...

Descripción completa

Detalles Bibliográficos
Autores principales: Yuan, Bowen, Liu, Tingting, Cheng, Ye, Gao, Shiqi, Li, Linzhi, Cai, Linna, Yang, Jian, Chen, Jianping, Zhong, Kaili
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8166574/
https://www.ncbi.nlm.nih.gov/pubmed/34084157
http://dx.doi.org/10.3389/fmicb.2021.672559
_version_ 1783701526723690496
author Yuan, Bowen
Liu, Tingting
Cheng, Ye
Gao, Shiqi
Li, Linzhi
Cai, Linna
Yang, Jian
Chen, Jianping
Zhong, Kaili
author_facet Yuan, Bowen
Liu, Tingting
Cheng, Ye
Gao, Shiqi
Li, Linzhi
Cai, Linna
Yang, Jian
Chen, Jianping
Zhong, Kaili
author_sort Yuan, Bowen
collection PubMed
description Protein lysine acetylation (Kac) is an important post-translational modification mechanism in eukaryotes that is involved in cellular regulation. To investigate the role of Kac in virus-infected plants, we characterized the lysine acetylome of Nicotiana benthamiana plants with or without a Chinese wheat mosaic virus (CWMV) infection. We identified 4,803 acetylated lysine sites on 1,964 proteins. A comparison of the acetylation levels of the CWMV-infected group with those of the uninfected group revealed that 747 sites were upregulated on 422 proteins, including chloroplast localization proteins and histone H3, and 150 sites were downregulated on 102 proteins. Nineteen conserved motifs were extracted and 51 percent of the acetylated proteins located on chloroplast. Nineteen Kac sites were located on histone proteins, including 10 Kac sites on histone 3. Bioinformatics analysis results indicated that lysine acetylation occurs on a large number of proteins involved in biological processes, especially photosynthesis. Furthermore, we found that the acetylation level of chloroplast proteins, histone 3 and some metabolic pathway-related proteins were significantly higher in CWMV-infected plants than in uninfected plants. In summary, our results reveal the regulatory roles of Kac in response to CWMV infection.
format Online
Article
Text
id pubmed-8166574
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-81665742021-06-02 Comprehensive Proteomic Analysis of Lysine Acetylation in Nicotiana benthamiana After Sensing CWMV Infection Yuan, Bowen Liu, Tingting Cheng, Ye Gao, Shiqi Li, Linzhi Cai, Linna Yang, Jian Chen, Jianping Zhong, Kaili Front Microbiol Microbiology Protein lysine acetylation (Kac) is an important post-translational modification mechanism in eukaryotes that is involved in cellular regulation. To investigate the role of Kac in virus-infected plants, we characterized the lysine acetylome of Nicotiana benthamiana plants with or without a Chinese wheat mosaic virus (CWMV) infection. We identified 4,803 acetylated lysine sites on 1,964 proteins. A comparison of the acetylation levels of the CWMV-infected group with those of the uninfected group revealed that 747 sites were upregulated on 422 proteins, including chloroplast localization proteins and histone H3, and 150 sites were downregulated on 102 proteins. Nineteen conserved motifs were extracted and 51 percent of the acetylated proteins located on chloroplast. Nineteen Kac sites were located on histone proteins, including 10 Kac sites on histone 3. Bioinformatics analysis results indicated that lysine acetylation occurs on a large number of proteins involved in biological processes, especially photosynthesis. Furthermore, we found that the acetylation level of chloroplast proteins, histone 3 and some metabolic pathway-related proteins were significantly higher in CWMV-infected plants than in uninfected plants. In summary, our results reveal the regulatory roles of Kac in response to CWMV infection. Frontiers Media S.A. 2021-05-17 /pmc/articles/PMC8166574/ /pubmed/34084157 http://dx.doi.org/10.3389/fmicb.2021.672559 Text en Copyright © 2021 Yuan, Liu, Cheng, Gao, Li, Cai, Yang, Chen and Zhong. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Yuan, Bowen
Liu, Tingting
Cheng, Ye
Gao, Shiqi
Li, Linzhi
Cai, Linna
Yang, Jian
Chen, Jianping
Zhong, Kaili
Comprehensive Proteomic Analysis of Lysine Acetylation in Nicotiana benthamiana After Sensing CWMV Infection
title Comprehensive Proteomic Analysis of Lysine Acetylation in Nicotiana benthamiana After Sensing CWMV Infection
title_full Comprehensive Proteomic Analysis of Lysine Acetylation in Nicotiana benthamiana After Sensing CWMV Infection
title_fullStr Comprehensive Proteomic Analysis of Lysine Acetylation in Nicotiana benthamiana After Sensing CWMV Infection
title_full_unstemmed Comprehensive Proteomic Analysis of Lysine Acetylation in Nicotiana benthamiana After Sensing CWMV Infection
title_short Comprehensive Proteomic Analysis of Lysine Acetylation in Nicotiana benthamiana After Sensing CWMV Infection
title_sort comprehensive proteomic analysis of lysine acetylation in nicotiana benthamiana after sensing cwmv infection
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8166574/
https://www.ncbi.nlm.nih.gov/pubmed/34084157
http://dx.doi.org/10.3389/fmicb.2021.672559
work_keys_str_mv AT yuanbowen comprehensiveproteomicanalysisoflysineacetylationinnicotianabenthamianaaftersensingcwmvinfection
AT liutingting comprehensiveproteomicanalysisoflysineacetylationinnicotianabenthamianaaftersensingcwmvinfection
AT chengye comprehensiveproteomicanalysisoflysineacetylationinnicotianabenthamianaaftersensingcwmvinfection
AT gaoshiqi comprehensiveproteomicanalysisoflysineacetylationinnicotianabenthamianaaftersensingcwmvinfection
AT lilinzhi comprehensiveproteomicanalysisoflysineacetylationinnicotianabenthamianaaftersensingcwmvinfection
AT cailinna comprehensiveproteomicanalysisoflysineacetylationinnicotianabenthamianaaftersensingcwmvinfection
AT yangjian comprehensiveproteomicanalysisoflysineacetylationinnicotianabenthamianaaftersensingcwmvinfection
AT chenjianping comprehensiveproteomicanalysisoflysineacetylationinnicotianabenthamianaaftersensingcwmvinfection
AT zhongkaili comprehensiveproteomicanalysisoflysineacetylationinnicotianabenthamianaaftersensingcwmvinfection