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The SOC1-like gene BoMADS50 is associated with the flowering of Bambusa oldhamii

Bamboo is known for its edible shoots and beautiful texture and has considerable economic and ornamental value. Unique among traditional flowering plants, many bamboo plants undergo extensive synchronized flowering followed by large-scale death, seriously affecting the productivity and application o...

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Autores principales: Hou, Dan, Li, Ling, Ma, Tengfei, Pei, Jialong, Zhao, Zhongyu, Lu, Mengzhu, Wu, Aimin, Lin, Xinchun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8166863/
https://www.ncbi.nlm.nih.gov/pubmed/34059654
http://dx.doi.org/10.1038/s41438-021-00557-4
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author Hou, Dan
Li, Ling
Ma, Tengfei
Pei, Jialong
Zhao, Zhongyu
Lu, Mengzhu
Wu, Aimin
Lin, Xinchun
author_facet Hou, Dan
Li, Ling
Ma, Tengfei
Pei, Jialong
Zhao, Zhongyu
Lu, Mengzhu
Wu, Aimin
Lin, Xinchun
author_sort Hou, Dan
collection PubMed
description Bamboo is known for its edible shoots and beautiful texture and has considerable economic and ornamental value. Unique among traditional flowering plants, many bamboo plants undergo extensive synchronized flowering followed by large-scale death, seriously affecting the productivity and application of bamboo forests. To date, the molecular mechanism of bamboo flowering characteristics has remained unknown. In this study, a SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1)-like gene, BoMADS50, was identified from Bambusa oldhamii. BoMADS50 was highly expressed in mature leaves and the floral primordium formation period during B. oldhamii flowering and overexpression of BoMADS50 caused early flowering in transgenic rice. Moreover, BoMADS50 could interact with APETALA1/FRUITFULL (AP1/FUL)-like proteins (BoMADS14-1/2, BoMADS15-1/2) in vivo, and the expression of BoMADS50 was significantly promoted by BoMADS14-1, further indicating a synergistic effect between BoMADS50 and BoAP1/FUL-like proteins in regulating B. oldhamii flowering. We also identified four additional transcripts of BoMADS50 (BoMADS50-1/2/3/4) with different nucleotide variations. Although the protein-CDS were polymorphic, they had flowering activation functions similar to those of BoMADS50. Yeast one-hybrid and transient expression assays subsequently showed that both BoMADS50 and BoMADS50-1 bind to the promoter fragment of itself and the SHORT VEGETATIVE PHASE (SVP)-like gene BoSVP, but only BoMADS50-1 can positively induce their transcription. Therefore, nucleotide variations likely endow BoMADS50-1 with strong regulatory activity. Thus, BoMADS50 and BoMADS50-1/2/3/4 are probably important positive flowering regulators in B. oldhamii. Moreover, the functional conservatism and specificity of BoMADS50 and BoMADS50-1 might be related to the synchronized and sporadic flowering characteristics of B. oldhamii.
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spelling pubmed-81668632021-06-15 The SOC1-like gene BoMADS50 is associated with the flowering of Bambusa oldhamii Hou, Dan Li, Ling Ma, Tengfei Pei, Jialong Zhao, Zhongyu Lu, Mengzhu Wu, Aimin Lin, Xinchun Hortic Res Article Bamboo is known for its edible shoots and beautiful texture and has considerable economic and ornamental value. Unique among traditional flowering plants, many bamboo plants undergo extensive synchronized flowering followed by large-scale death, seriously affecting the productivity and application of bamboo forests. To date, the molecular mechanism of bamboo flowering characteristics has remained unknown. In this study, a SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1)-like gene, BoMADS50, was identified from Bambusa oldhamii. BoMADS50 was highly expressed in mature leaves and the floral primordium formation period during B. oldhamii flowering and overexpression of BoMADS50 caused early flowering in transgenic rice. Moreover, BoMADS50 could interact with APETALA1/FRUITFULL (AP1/FUL)-like proteins (BoMADS14-1/2, BoMADS15-1/2) in vivo, and the expression of BoMADS50 was significantly promoted by BoMADS14-1, further indicating a synergistic effect between BoMADS50 and BoAP1/FUL-like proteins in regulating B. oldhamii flowering. We also identified four additional transcripts of BoMADS50 (BoMADS50-1/2/3/4) with different nucleotide variations. Although the protein-CDS were polymorphic, they had flowering activation functions similar to those of BoMADS50. Yeast one-hybrid and transient expression assays subsequently showed that both BoMADS50 and BoMADS50-1 bind to the promoter fragment of itself and the SHORT VEGETATIVE PHASE (SVP)-like gene BoSVP, but only BoMADS50-1 can positively induce their transcription. Therefore, nucleotide variations likely endow BoMADS50-1 with strong regulatory activity. Thus, BoMADS50 and BoMADS50-1/2/3/4 are probably important positive flowering regulators in B. oldhamii. Moreover, the functional conservatism and specificity of BoMADS50 and BoMADS50-1 might be related to the synchronized and sporadic flowering characteristics of B. oldhamii. Nature Publishing Group UK 2021-06-01 /pmc/articles/PMC8166863/ /pubmed/34059654 http://dx.doi.org/10.1038/s41438-021-00557-4 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Hou, Dan
Li, Ling
Ma, Tengfei
Pei, Jialong
Zhao, Zhongyu
Lu, Mengzhu
Wu, Aimin
Lin, Xinchun
The SOC1-like gene BoMADS50 is associated with the flowering of Bambusa oldhamii
title The SOC1-like gene BoMADS50 is associated with the flowering of Bambusa oldhamii
title_full The SOC1-like gene BoMADS50 is associated with the flowering of Bambusa oldhamii
title_fullStr The SOC1-like gene BoMADS50 is associated with the flowering of Bambusa oldhamii
title_full_unstemmed The SOC1-like gene BoMADS50 is associated with the flowering of Bambusa oldhamii
title_short The SOC1-like gene BoMADS50 is associated with the flowering of Bambusa oldhamii
title_sort soc1-like gene bomads50 is associated with the flowering of bambusa oldhamii
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8166863/
https://www.ncbi.nlm.nih.gov/pubmed/34059654
http://dx.doi.org/10.1038/s41438-021-00557-4
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