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SlRCM1, which encodes tomato Lutescent1, is required for chlorophyll synthesis and chloroplast development in fruits

In plants, chloroplasts are the sites at which photosynthesis occurs, and an increased abundance of chloroplasts increases the nutritional quality of plants and the resultant color of fruits. However, the molecular mechanisms underlying chlorophyll synthesis and chloroplast development in tomato fru...

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Autores principales: Liu, Genzhong, Yu, Huiyang, Yuan, Lei, Li, Changxing, Ye, Jie, Chen, Weifang, Wang, Ying, Ge, Pingfei, Zhang, Junhong, Ye, Zhibiao, Zhang, Yuyang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8166902/
https://www.ncbi.nlm.nih.gov/pubmed/34059638
http://dx.doi.org/10.1038/s41438-021-00563-6
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author Liu, Genzhong
Yu, Huiyang
Yuan, Lei
Li, Changxing
Ye, Jie
Chen, Weifang
Wang, Ying
Ge, Pingfei
Zhang, Junhong
Ye, Zhibiao
Zhang, Yuyang
author_facet Liu, Genzhong
Yu, Huiyang
Yuan, Lei
Li, Changxing
Ye, Jie
Chen, Weifang
Wang, Ying
Ge, Pingfei
Zhang, Junhong
Ye, Zhibiao
Zhang, Yuyang
author_sort Liu, Genzhong
collection PubMed
description In plants, chloroplasts are the sites at which photosynthesis occurs, and an increased abundance of chloroplasts increases the nutritional quality of plants and the resultant color of fruits. However, the molecular mechanisms underlying chlorophyll synthesis and chloroplast development in tomato fruits remain unknown. In this study, we isolated a chlorophyll-deficient mutant, reduced chlorophyll mutant 1 (rcm1), by ethylmethanesulfonate mutagenesis; this mutant produced yellowish fruits with altered chloroplast development. MutMap revealed that Solyc08g005010 is the causal gene underlying the rcm1 mutant phenotype. A single-nucleotide base substitution in the second exon of SlRCM1 results in premature termination of its translated protein. SlRCM1 encodes a chloroplast-targeted metalloendopeptidase that is orthologous to the BCM1 protein of Arabidopsis and the stay-green G protein of soybean (Glycine max L. Merr.). Notably, the yellowish phenotype of the lutescent1 mutant can be restored with the allele of SlRCM1 from wild-type tomato. In contrast, knockout of SlRCM1 by the CRISPR/Cas9 system in Alisa Craig yielded yellowish fruits at the mature green stage, as was the case for lutescent1. Amino acid sequence alignment and functional complementation assays showed that SlRCM1 is indeed Lutescent1. These findings provide new insights into the regulation of chloroplast development in tomato fruits.
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spelling pubmed-81669022021-06-07 SlRCM1, which encodes tomato Lutescent1, is required for chlorophyll synthesis and chloroplast development in fruits Liu, Genzhong Yu, Huiyang Yuan, Lei Li, Changxing Ye, Jie Chen, Weifang Wang, Ying Ge, Pingfei Zhang, Junhong Ye, Zhibiao Zhang, Yuyang Hortic Res Article In plants, chloroplasts are the sites at which photosynthesis occurs, and an increased abundance of chloroplasts increases the nutritional quality of plants and the resultant color of fruits. However, the molecular mechanisms underlying chlorophyll synthesis and chloroplast development in tomato fruits remain unknown. In this study, we isolated a chlorophyll-deficient mutant, reduced chlorophyll mutant 1 (rcm1), by ethylmethanesulfonate mutagenesis; this mutant produced yellowish fruits with altered chloroplast development. MutMap revealed that Solyc08g005010 is the causal gene underlying the rcm1 mutant phenotype. A single-nucleotide base substitution in the second exon of SlRCM1 results in premature termination of its translated protein. SlRCM1 encodes a chloroplast-targeted metalloendopeptidase that is orthologous to the BCM1 protein of Arabidopsis and the stay-green G protein of soybean (Glycine max L. Merr.). Notably, the yellowish phenotype of the lutescent1 mutant can be restored with the allele of SlRCM1 from wild-type tomato. In contrast, knockout of SlRCM1 by the CRISPR/Cas9 system in Alisa Craig yielded yellowish fruits at the mature green stage, as was the case for lutescent1. Amino acid sequence alignment and functional complementation assays showed that SlRCM1 is indeed Lutescent1. These findings provide new insights into the regulation of chloroplast development in tomato fruits. Nature Publishing Group UK 2021-06-01 /pmc/articles/PMC8166902/ /pubmed/34059638 http://dx.doi.org/10.1038/s41438-021-00563-6 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Liu, Genzhong
Yu, Huiyang
Yuan, Lei
Li, Changxing
Ye, Jie
Chen, Weifang
Wang, Ying
Ge, Pingfei
Zhang, Junhong
Ye, Zhibiao
Zhang, Yuyang
SlRCM1, which encodes tomato Lutescent1, is required for chlorophyll synthesis and chloroplast development in fruits
title SlRCM1, which encodes tomato Lutescent1, is required for chlorophyll synthesis and chloroplast development in fruits
title_full SlRCM1, which encodes tomato Lutescent1, is required for chlorophyll synthesis and chloroplast development in fruits
title_fullStr SlRCM1, which encodes tomato Lutescent1, is required for chlorophyll synthesis and chloroplast development in fruits
title_full_unstemmed SlRCM1, which encodes tomato Lutescent1, is required for chlorophyll synthesis and chloroplast development in fruits
title_short SlRCM1, which encodes tomato Lutescent1, is required for chlorophyll synthesis and chloroplast development in fruits
title_sort slrcm1, which encodes tomato lutescent1, is required for chlorophyll synthesis and chloroplast development in fruits
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8166902/
https://www.ncbi.nlm.nih.gov/pubmed/34059638
http://dx.doi.org/10.1038/s41438-021-00563-6
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