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Multi-species and multi-tissue methylation clocks for age estimation in toothed whales and dolphins
The development of a precise blood or skin tissue DNA Epigenetic Aging Clock for Odontocete (OEAC) would solve current age estimation inaccuracies for wild odontocetes. Therefore, we determined genome-wide DNA methylation profiles using a custom array (HorvathMammalMethyl40) across skin and blood sa...
| Autores principales: | , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Nature Publishing Group UK
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167141/ https://www.ncbi.nlm.nih.gov/pubmed/34059764 http://dx.doi.org/10.1038/s42003-021-02179-x |
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| author | Robeck, Todd R. Fei, Zhe Lu, Ake T. Haghani, Amin Jourdain, Eve Zoller, Joseph A. Li, Caesar Z. Steinman, Karen J. DiRocco, Stacy Schmitt, Todd Osborn, Steve Van Bonn, Bill Katsumata, Etsuko Mergl, June Almunia, Javier Rodriguez, Magdalena Haulena, Martin Dold, Christopher Horvath, Steve |
| author_facet | Robeck, Todd R. Fei, Zhe Lu, Ake T. Haghani, Amin Jourdain, Eve Zoller, Joseph A. Li, Caesar Z. Steinman, Karen J. DiRocco, Stacy Schmitt, Todd Osborn, Steve Van Bonn, Bill Katsumata, Etsuko Mergl, June Almunia, Javier Rodriguez, Magdalena Haulena, Martin Dold, Christopher Horvath, Steve |
| author_sort | Robeck, Todd R. |
| collection | PubMed |
| description | The development of a precise blood or skin tissue DNA Epigenetic Aging Clock for Odontocete (OEAC) would solve current age estimation inaccuracies for wild odontocetes. Therefore, we determined genome-wide DNA methylation profiles using a custom array (HorvathMammalMethyl40) across skin and blood samples (n = 446) from known age animals representing nine odontocete species within 4 phylogenetic families to identify age associated CG dinucleotides (CpGs). The top CpGs were used to create a cross-validated OEAC clock which was highly correlated for individuals (r = 0.94) and for unique species (median r = 0.93). Finally, we applied the OEAC for estimating the age and sex of 22 wild Norwegian killer whales. DNA methylation patterns of age associated CpGs are highly conserved across odontocetes. These similarities allowed us to develop an odontocete epigenetic aging clock (OEAC) which can be used for species conservation efforts by provide a mechanism for estimating the age of free ranging odontocetes from either blood or skin samples. |
| format | Online Article Text |
| id | pubmed-8167141 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2021 |
| publisher | Nature Publishing Group UK |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-81671412021-06-07 Multi-species and multi-tissue methylation clocks for age estimation in toothed whales and dolphins Robeck, Todd R. Fei, Zhe Lu, Ake T. Haghani, Amin Jourdain, Eve Zoller, Joseph A. Li, Caesar Z. Steinman, Karen J. DiRocco, Stacy Schmitt, Todd Osborn, Steve Van Bonn, Bill Katsumata, Etsuko Mergl, June Almunia, Javier Rodriguez, Magdalena Haulena, Martin Dold, Christopher Horvath, Steve Commun Biol Article The development of a precise blood or skin tissue DNA Epigenetic Aging Clock for Odontocete (OEAC) would solve current age estimation inaccuracies for wild odontocetes. Therefore, we determined genome-wide DNA methylation profiles using a custom array (HorvathMammalMethyl40) across skin and blood samples (n = 446) from known age animals representing nine odontocete species within 4 phylogenetic families to identify age associated CG dinucleotides (CpGs). The top CpGs were used to create a cross-validated OEAC clock which was highly correlated for individuals (r = 0.94) and for unique species (median r = 0.93). Finally, we applied the OEAC for estimating the age and sex of 22 wild Norwegian killer whales. DNA methylation patterns of age associated CpGs are highly conserved across odontocetes. These similarities allowed us to develop an odontocete epigenetic aging clock (OEAC) which can be used for species conservation efforts by provide a mechanism for estimating the age of free ranging odontocetes from either blood or skin samples. Nature Publishing Group UK 2021-05-31 /pmc/articles/PMC8167141/ /pubmed/34059764 http://dx.doi.org/10.1038/s42003-021-02179-x Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
| spellingShingle | Article Robeck, Todd R. Fei, Zhe Lu, Ake T. Haghani, Amin Jourdain, Eve Zoller, Joseph A. Li, Caesar Z. Steinman, Karen J. DiRocco, Stacy Schmitt, Todd Osborn, Steve Van Bonn, Bill Katsumata, Etsuko Mergl, June Almunia, Javier Rodriguez, Magdalena Haulena, Martin Dold, Christopher Horvath, Steve Multi-species and multi-tissue methylation clocks for age estimation in toothed whales and dolphins |
| title | Multi-species and multi-tissue methylation clocks for age estimation in toothed whales and dolphins |
| title_full | Multi-species and multi-tissue methylation clocks for age estimation in toothed whales and dolphins |
| title_fullStr | Multi-species and multi-tissue methylation clocks for age estimation in toothed whales and dolphins |
| title_full_unstemmed | Multi-species and multi-tissue methylation clocks for age estimation in toothed whales and dolphins |
| title_short | Multi-species and multi-tissue methylation clocks for age estimation in toothed whales and dolphins |
| title_sort | multi-species and multi-tissue methylation clocks for age estimation in toothed whales and dolphins |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167141/ https://www.ncbi.nlm.nih.gov/pubmed/34059764 http://dx.doi.org/10.1038/s42003-021-02179-x |
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