Cargando…

Neuroproteomics of the Synapse: Subcellular Quantification of Protein Networks and Signaling Dynamics

One of the most fascinating features of the brain is its ability to adapt to its surroundings. Synaptic plasticity, the dynamic mechanism of functional and structural alterations in synaptic strength, is essential for brain functioning and underlies a variety of processes such as learning and memory...

Descripción completa

Detalles Bibliográficos
Autores principales: van Gelder, Charlotte A.G.H., Altelaar, Maarten
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167277/
https://www.ncbi.nlm.nih.gov/pubmed/33933679
http://dx.doi.org/10.1016/j.mcpro.2021.100087
_version_ 1783701661724704768
author van Gelder, Charlotte A.G.H.
Altelaar, Maarten
author_facet van Gelder, Charlotte A.G.H.
Altelaar, Maarten
author_sort van Gelder, Charlotte A.G.H.
collection PubMed
description One of the most fascinating features of the brain is its ability to adapt to its surroundings. Synaptic plasticity, the dynamic mechanism of functional and structural alterations in synaptic strength, is essential for brain functioning and underlies a variety of processes such as learning and memory. Although the molecular mechanisms underlying such rapid plasticity are not fully understood, a consensus exists on the important role of proteins. The study of these neuronal proteins using neuroproteomics has increased rapidly in the last decades, and advancements in MS-based proteomics have broadened our understanding of neuroplasticity exponentially. In this review, we discuss the trends in MS-based neuroproteomics for the study of synaptic protein–protein interactions and protein signaling dynamics, with a focus on sample types, different labeling and enrichment approaches, and data analysis and interpretation. We highlight studies from the last 5 years, with a focus on synapse structure, composition, functioning, or signaling and finally discuss some recent developments that could further advance the field of neuroproteomics.
format Online
Article
Text
id pubmed-8167277
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher American Society for Biochemistry and Molecular Biology
record_format MEDLINE/PubMed
spelling pubmed-81672772021-06-05 Neuroproteomics of the Synapse: Subcellular Quantification of Protein Networks and Signaling Dynamics van Gelder, Charlotte A.G.H. Altelaar, Maarten Mol Cell Proteomics Review One of the most fascinating features of the brain is its ability to adapt to its surroundings. Synaptic plasticity, the dynamic mechanism of functional and structural alterations in synaptic strength, is essential for brain functioning and underlies a variety of processes such as learning and memory. Although the molecular mechanisms underlying such rapid plasticity are not fully understood, a consensus exists on the important role of proteins. The study of these neuronal proteins using neuroproteomics has increased rapidly in the last decades, and advancements in MS-based proteomics have broadened our understanding of neuroplasticity exponentially. In this review, we discuss the trends in MS-based neuroproteomics for the study of synaptic protein–protein interactions and protein signaling dynamics, with a focus on sample types, different labeling and enrichment approaches, and data analysis and interpretation. We highlight studies from the last 5 years, with a focus on synapse structure, composition, functioning, or signaling and finally discuss some recent developments that could further advance the field of neuroproteomics. American Society for Biochemistry and Molecular Biology 2021-04-29 /pmc/articles/PMC8167277/ /pubmed/33933679 http://dx.doi.org/10.1016/j.mcpro.2021.100087 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
van Gelder, Charlotte A.G.H.
Altelaar, Maarten
Neuroproteomics of the Synapse: Subcellular Quantification of Protein Networks and Signaling Dynamics
title Neuroproteomics of the Synapse: Subcellular Quantification of Protein Networks and Signaling Dynamics
title_full Neuroproteomics of the Synapse: Subcellular Quantification of Protein Networks and Signaling Dynamics
title_fullStr Neuroproteomics of the Synapse: Subcellular Quantification of Protein Networks and Signaling Dynamics
title_full_unstemmed Neuroproteomics of the Synapse: Subcellular Quantification of Protein Networks and Signaling Dynamics
title_short Neuroproteomics of the Synapse: Subcellular Quantification of Protein Networks and Signaling Dynamics
title_sort neuroproteomics of the synapse: subcellular quantification of protein networks and signaling dynamics
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167277/
https://www.ncbi.nlm.nih.gov/pubmed/33933679
http://dx.doi.org/10.1016/j.mcpro.2021.100087
work_keys_str_mv AT vangeldercharlotteagh neuroproteomicsofthesynapsesubcellularquantificationofproteinnetworksandsignalingdynamics
AT altelaarmaarten neuroproteomicsofthesynapsesubcellularquantificationofproteinnetworksandsignalingdynamics