The quorum sensing regulator OpaR is a repressor of polar flagellum genes in Vibrio parahaemolyticus

Vibrio parahaemolyticus possesses two types of flagella: a single polar flagellum (Pof) for swimming and the peritrichous lateral flagella (Laf) for swarming. Expression of Laf genes has previously been reported to be regulated by the quorum sensing (QS) regulators AphA and OpaR. In the present stud...

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Autores principales: Lu, Renfei, Sun, Junfang, Qiu, Yue, Zhang, Miaomiao, Xue, Xingfan, Li, Xue, Yang, Wenhui, Zhou, Dongsheng, Hu, Lingfei, Zhang, Yiquan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Microbiological Society of Korea 2021
Materias:
Microbial Genetics, Genomics and Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167305/
https://www.ncbi.nlm.nih.gov/pubmed/34061340
http://dx.doi.org/10.1007/s12275-021-0629-3
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author Lu, Renfei
Sun, Junfang
Qiu, Yue
Zhang, Miaomiao
Xue, Xingfan
Li, Xue
Yang, Wenhui
Zhou, Dongsheng
Hu, Lingfei
Zhang, Yiquan
author_facet Lu, Renfei
Sun, Junfang
Qiu, Yue
Zhang, Miaomiao
Xue, Xingfan
Li, Xue
Yang, Wenhui
Zhou, Dongsheng
Hu, Lingfei
Zhang, Yiquan
author_sort Lu, Renfei
collection PubMed
description Vibrio parahaemolyticus possesses two types of flagella: a single polar flagellum (Pof) for swimming and the peritrichous lateral flagella (Laf) for swarming. Expression of Laf genes has previously been reported to be regulated by the quorum sensing (QS) regulators AphA and OpaR. In the present study, we showed that OpaR, the QS regulator at high cell density (HCD), acted as a negative regulator of swimming motility and the transcription of Pof genes in V. parahaemolyticus. OpaR bound to the promoter-proximal DNA regions of flgAMN, flgMN, and flgBCDEFGHIJ within the Pof gene loci to repress their transcription, whereas it negatively regulates the transcription of flgKL-flaC in an indirect manner. Thus, this work investigated how QS regulated the swimming motility via direct action of its master regulator OpaR on the transcription of Pof genes in V. parahaemolyticus.
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spelling pubmed-81673052021-06-01 The quorum sensing regulator OpaR is a repressor of polar flagellum genes in Vibrio parahaemolyticus Lu, Renfei Sun, Junfang Qiu, Yue Zhang, Miaomiao Xue, Xingfan Li, Xue Yang, Wenhui Zhou, Dongsheng Hu, Lingfei Zhang, Yiquan J Microbiol Microbial Genetics, Genomics and Molecular Biology Vibrio parahaemolyticus possesses two types of flagella: a single polar flagellum (Pof) for swimming and the peritrichous lateral flagella (Laf) for swarming. Expression of Laf genes has previously been reported to be regulated by the quorum sensing (QS) regulators AphA and OpaR. In the present study, we showed that OpaR, the QS regulator at high cell density (HCD), acted as a negative regulator of swimming motility and the transcription of Pof genes in V. parahaemolyticus. OpaR bound to the promoter-proximal DNA regions of flgAMN, flgMN, and flgBCDEFGHIJ within the Pof gene loci to repress their transcription, whereas it negatively regulates the transcription of flgKL-flaC in an indirect manner. Thus, this work investigated how QS regulated the swimming motility via direct action of its master regulator OpaR on the transcription of Pof genes in V. parahaemolyticus. The Microbiological Society of Korea 2021-06-01 2021 /pmc/articles/PMC8167305/ /pubmed/34061340 http://dx.doi.org/10.1007/s12275-021-0629-3 Text en © The Microbiological Society of Korea 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Microbial Genetics, Genomics and Molecular Biology
Lu, Renfei
Sun, Junfang
Qiu, Yue
Zhang, Miaomiao
Xue, Xingfan
Li, Xue
Yang, Wenhui
Zhou, Dongsheng
Hu, Lingfei
Zhang, Yiquan
The quorum sensing regulator OpaR is a repressor of polar flagellum genes in Vibrio parahaemolyticus
title The quorum sensing regulator OpaR is a repressor of polar flagellum genes in Vibrio parahaemolyticus
title_full The quorum sensing regulator OpaR is a repressor of polar flagellum genes in Vibrio parahaemolyticus
title_fullStr The quorum sensing regulator OpaR is a repressor of polar flagellum genes in Vibrio parahaemolyticus
title_full_unstemmed The quorum sensing regulator OpaR is a repressor of polar flagellum genes in Vibrio parahaemolyticus
title_short The quorum sensing regulator OpaR is a repressor of polar flagellum genes in Vibrio parahaemolyticus
title_sort quorum sensing regulator opar is a repressor of polar flagellum genes in vibrio parahaemolyticus
topic Microbial Genetics, Genomics and Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167305/
https://www.ncbi.nlm.nih.gov/pubmed/34061340
http://dx.doi.org/10.1007/s12275-021-0629-3
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