Cargando…

Double-Stranded RNA Targeting Dicer-Like Genes Compromises the Pathogenicity of Plasmopara viticola on Grapevine

Downy mildew caused by Plasmopara viticola is one of the most devastating diseases of grapevine, attacking all green parts of the plant. The damage is severe when the infection at flowering stage is left uncontrolled. P. viticola management consumes a significant amount of classical pesticides appli...

Descripción completa

Detalles Bibliográficos
Autores principales: Haile, Zeraye Mehari, Gebremichael, Daniel Endale, Capriotti, Luca, Molesini, Barbara, Negrini, Francesca, Collina, Marina, Sabbadini, Silvia, Mezzetti, Bruno, Baraldi, Elena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167485/
https://www.ncbi.nlm.nih.gov/pubmed/34084177
http://dx.doi.org/10.3389/fpls.2021.667539
_version_ 1783701701815959552
author Haile, Zeraye Mehari
Gebremichael, Daniel Endale
Capriotti, Luca
Molesini, Barbara
Negrini, Francesca
Collina, Marina
Sabbadini, Silvia
Mezzetti, Bruno
Baraldi, Elena
author_facet Haile, Zeraye Mehari
Gebremichael, Daniel Endale
Capriotti, Luca
Molesini, Barbara
Negrini, Francesca
Collina, Marina
Sabbadini, Silvia
Mezzetti, Bruno
Baraldi, Elena
author_sort Haile, Zeraye Mehari
collection PubMed
description Downy mildew caused by Plasmopara viticola is one of the most devastating diseases of grapevine, attacking all green parts of the plant. The damage is severe when the infection at flowering stage is left uncontrolled. P. viticola management consumes a significant amount of classical pesticides applied in vineyards, requiring efficient and environmentally safe disease management options. Spray-induced gene silencing (SIGS), through the application of exogenous double-stranded RNA (dsRNA), has shown promising results for the management of diseases in crops. Here, we developed and tested the potential of dsRNA targeting P. viticola Dicer-like (DCL) genes for SIGS-based crop protection strategy. The exogenous application of PvDCL1/2 dsRNA, a chimera of PvDCL1 and PvDCL2, highly affected the virulence of P. viticola. The reduced expression level of PvDCL1 and PvDCL2 transcripts in infected leaves, treated with PvDCL1/2 dsRNA, was an indication of an active RNA interference mechanism inside the pathogen to compromise its virulence. Besides the protective property, the PvDCL1/2 dsRNA also exhibited a curative role by reducing the disease progress rate of already established infection. Our data provide a promising future for PvDCL1/2 dsRNA as a new generation of RNA-based resistant plants or RNA-based agrochemical for the management of downy mildew disease in grapevine.
format Online
Article
Text
id pubmed-8167485
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-81674852021-06-02 Double-Stranded RNA Targeting Dicer-Like Genes Compromises the Pathogenicity of Plasmopara viticola on Grapevine Haile, Zeraye Mehari Gebremichael, Daniel Endale Capriotti, Luca Molesini, Barbara Negrini, Francesca Collina, Marina Sabbadini, Silvia Mezzetti, Bruno Baraldi, Elena Front Plant Sci Plant Science Downy mildew caused by Plasmopara viticola is one of the most devastating diseases of grapevine, attacking all green parts of the plant. The damage is severe when the infection at flowering stage is left uncontrolled. P. viticola management consumes a significant amount of classical pesticides applied in vineyards, requiring efficient and environmentally safe disease management options. Spray-induced gene silencing (SIGS), through the application of exogenous double-stranded RNA (dsRNA), has shown promising results for the management of diseases in crops. Here, we developed and tested the potential of dsRNA targeting P. viticola Dicer-like (DCL) genes for SIGS-based crop protection strategy. The exogenous application of PvDCL1/2 dsRNA, a chimera of PvDCL1 and PvDCL2, highly affected the virulence of P. viticola. The reduced expression level of PvDCL1 and PvDCL2 transcripts in infected leaves, treated with PvDCL1/2 dsRNA, was an indication of an active RNA interference mechanism inside the pathogen to compromise its virulence. Besides the protective property, the PvDCL1/2 dsRNA also exhibited a curative role by reducing the disease progress rate of already established infection. Our data provide a promising future for PvDCL1/2 dsRNA as a new generation of RNA-based resistant plants or RNA-based agrochemical for the management of downy mildew disease in grapevine. Frontiers Media S.A. 2021-05-18 /pmc/articles/PMC8167485/ /pubmed/34084177 http://dx.doi.org/10.3389/fpls.2021.667539 Text en Copyright © 2021 Haile, Gebremichael, Capriotti, Molesini, Negrini, Collina, Sabbadini, Mezzetti and Baraldi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Haile, Zeraye Mehari
Gebremichael, Daniel Endale
Capriotti, Luca
Molesini, Barbara
Negrini, Francesca
Collina, Marina
Sabbadini, Silvia
Mezzetti, Bruno
Baraldi, Elena
Double-Stranded RNA Targeting Dicer-Like Genes Compromises the Pathogenicity of Plasmopara viticola on Grapevine
title Double-Stranded RNA Targeting Dicer-Like Genes Compromises the Pathogenicity of Plasmopara viticola on Grapevine
title_full Double-Stranded RNA Targeting Dicer-Like Genes Compromises the Pathogenicity of Plasmopara viticola on Grapevine
title_fullStr Double-Stranded RNA Targeting Dicer-Like Genes Compromises the Pathogenicity of Plasmopara viticola on Grapevine
title_full_unstemmed Double-Stranded RNA Targeting Dicer-Like Genes Compromises the Pathogenicity of Plasmopara viticola on Grapevine
title_short Double-Stranded RNA Targeting Dicer-Like Genes Compromises the Pathogenicity of Plasmopara viticola on Grapevine
title_sort double-stranded rna targeting dicer-like genes compromises the pathogenicity of plasmopara viticola on grapevine
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167485/
https://www.ncbi.nlm.nih.gov/pubmed/34084177
http://dx.doi.org/10.3389/fpls.2021.667539
work_keys_str_mv AT hailezerayemehari doublestrandedrnatargetingdicerlikegenescompromisesthepathogenicityofplasmoparaviticolaongrapevine
AT gebremichaeldanielendale doublestrandedrnatargetingdicerlikegenescompromisesthepathogenicityofplasmoparaviticolaongrapevine
AT capriottiluca doublestrandedrnatargetingdicerlikegenescompromisesthepathogenicityofplasmoparaviticolaongrapevine
AT molesinibarbara doublestrandedrnatargetingdicerlikegenescompromisesthepathogenicityofplasmoparaviticolaongrapevine
AT negrinifrancesca doublestrandedrnatargetingdicerlikegenescompromisesthepathogenicityofplasmoparaviticolaongrapevine
AT collinamarina doublestrandedrnatargetingdicerlikegenescompromisesthepathogenicityofplasmoparaviticolaongrapevine
AT sabbadinisilvia doublestrandedrnatargetingdicerlikegenescompromisesthepathogenicityofplasmoparaviticolaongrapevine
AT mezzettibruno doublestrandedrnatargetingdicerlikegenescompromisesthepathogenicityofplasmoparaviticolaongrapevine
AT baraldielena doublestrandedrnatargetingdicerlikegenescompromisesthepathogenicityofplasmoparaviticolaongrapevine