Cargando…

Detection of Pestivirus in small ruminants in Central Java, Indonesia

BACKGROUND AND AIM: Globally, pestiviruses are among the most economically important viral pathogens of livestock. The genus Pestivirus comprises four species, including bovine viral diarrhea virus type 1 and 2 (BVDV-1 and BVDV-2), which infect cattle, border disease virus and classical swine fever...

Descripción completa

Detalles Bibliográficos
Autores principales: Hidayat, W., Wuryastuty, H., Wasito, R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167512/
https://www.ncbi.nlm.nih.gov/pubmed/34083951
http://dx.doi.org/10.14202/vetworld.2021.996-1001
_version_ 1783701704911355904
author Hidayat, W.
Wuryastuty, H.
Wasito, R.
author_facet Hidayat, W.
Wuryastuty, H.
Wasito, R.
author_sort Hidayat, W.
collection PubMed
description BACKGROUND AND AIM: Globally, pestiviruses are among the most economically important viral pathogens of livestock. The genus Pestivirus comprises four species, including bovine viral diarrhea virus type 1 and 2 (BVDV-1 and BVDV-2), which infect cattle, border disease virus and classical swine fever virus which infect small ruminants and pigs, respectively. Accumulating evidence suggests that pestiviruses are no longer species-specific, creating new challenges for disease control. In Indonesia, investigations related to pestiviruses remain focused on cattle as the primary host and no research has been conducted on small ruminants (sheep and goats). Therefore, the present study aimed to study the possible occurrence of pestivirus (BVDV or BVD) infections in small ruminants in Indonesia, particularly in Central Java. MATERIALS AND METHODS: We used 46 blood samples consisting of 26 sheep’s blood and 20 goat’s blood. Samples were selected from 247 small ruminant blood collected between July and October 2020 in Central Java, Indonesia, which met the following criteria: Female, local species, approximately 1-2 years old, never been pregnant, raised in the backyard, and had no close contact with cattle in either shelter or grazing area. We tested plasma samples from sheep and goats using competitive antibody enzyme-linked immunosorbent assay to detect specific antibodies against pestivirus followed by reverse transcription-polymerase chain reaction (RT-PCR) analysis for all positive samples to differentiate the species of pestivirus. RESULTS: Two of the 20 samples collected from goats were positive for pestivirus at the serological and molecular levels, whereas 2 of 26 samples collected from sheep were doubtful but tested negative by RT-PCR. The genotyping test results obtained using nested PCR revealed that the positive samples collected from goats had a BVDV-1 genotype. CONCLUSION: The results of the present study demonstrated that BVDV-1 can infect species other than bovines, in Central Java, Indonesia. Further studies involving a larger number of samples are required to: (1) Determine the actual seroprevalence of pestiviruses in small ruminants and (2) Determine the potency of small ruminants as reservoirs for pestiviruses, both of which are important for the identification of the appropriate control program for pestiviruses in Indonesia.
format Online
Article
Text
id pubmed-8167512
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Veterinary World
record_format MEDLINE/PubMed
spelling pubmed-81675122021-06-02 Detection of Pestivirus in small ruminants in Central Java, Indonesia Hidayat, W. Wuryastuty, H. Wasito, R. Vet World Research Article BACKGROUND AND AIM: Globally, pestiviruses are among the most economically important viral pathogens of livestock. The genus Pestivirus comprises four species, including bovine viral diarrhea virus type 1 and 2 (BVDV-1 and BVDV-2), which infect cattle, border disease virus and classical swine fever virus which infect small ruminants and pigs, respectively. Accumulating evidence suggests that pestiviruses are no longer species-specific, creating new challenges for disease control. In Indonesia, investigations related to pestiviruses remain focused on cattle as the primary host and no research has been conducted on small ruminants (sheep and goats). Therefore, the present study aimed to study the possible occurrence of pestivirus (BVDV or BVD) infections in small ruminants in Indonesia, particularly in Central Java. MATERIALS AND METHODS: We used 46 blood samples consisting of 26 sheep’s blood and 20 goat’s blood. Samples were selected from 247 small ruminant blood collected between July and October 2020 in Central Java, Indonesia, which met the following criteria: Female, local species, approximately 1-2 years old, never been pregnant, raised in the backyard, and had no close contact with cattle in either shelter or grazing area. We tested plasma samples from sheep and goats using competitive antibody enzyme-linked immunosorbent assay to detect specific antibodies against pestivirus followed by reverse transcription-polymerase chain reaction (RT-PCR) analysis for all positive samples to differentiate the species of pestivirus. RESULTS: Two of the 20 samples collected from goats were positive for pestivirus at the serological and molecular levels, whereas 2 of 26 samples collected from sheep were doubtful but tested negative by RT-PCR. The genotyping test results obtained using nested PCR revealed that the positive samples collected from goats had a BVDV-1 genotype. CONCLUSION: The results of the present study demonstrated that BVDV-1 can infect species other than bovines, in Central Java, Indonesia. Further studies involving a larger number of samples are required to: (1) Determine the actual seroprevalence of pestiviruses in small ruminants and (2) Determine the potency of small ruminants as reservoirs for pestiviruses, both of which are important for the identification of the appropriate control program for pestiviruses in Indonesia. Veterinary World 2021-04 2021-04-24 /pmc/articles/PMC8167512/ /pubmed/34083951 http://dx.doi.org/10.14202/vetworld.2021.996-1001 Text en Copyright: © Hidayat, et al. https://creativecommons.org/licenses/by/4.0/Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Hidayat, W.
Wuryastuty, H.
Wasito, R.
Detection of Pestivirus in small ruminants in Central Java, Indonesia
title Detection of Pestivirus in small ruminants in Central Java, Indonesia
title_full Detection of Pestivirus in small ruminants in Central Java, Indonesia
title_fullStr Detection of Pestivirus in small ruminants in Central Java, Indonesia
title_full_unstemmed Detection of Pestivirus in small ruminants in Central Java, Indonesia
title_short Detection of Pestivirus in small ruminants in Central Java, Indonesia
title_sort detection of pestivirus in small ruminants in central java, indonesia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167512/
https://www.ncbi.nlm.nih.gov/pubmed/34083951
http://dx.doi.org/10.14202/vetworld.2021.996-1001
work_keys_str_mv AT hidayatw detectionofpestivirusinsmallruminantsincentraljavaindonesia
AT wuryastutyh detectionofpestivirusinsmallruminantsincentraljavaindonesia
AT wasitor detectionofpestivirusinsmallruminantsincentraljavaindonesia