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Electroporation of Mouse Follicles, Oocytes and Embryos without Manipulating Zona Pellucida

Electroporation is an effective technique of transfection, but its efficiency depends on the optimization of various parameters. In this study, a simplified and efficient method of gene manipulation was standardized through electroporation to introduce a recombinant green fluorescent protein (GFP) c...

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Detalles Bibliográficos
Autores principales: Hakim, Bilal Ahmad, Tyagi, Vaishali, Agnihotri, Saurabh Kumar, Nath, Amar, Agrawal, Ankit Kumar, Jain, Ankita, Singh, Deependra, Konwar, Rituraj, Sachdev, Monika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167572/
https://www.ncbi.nlm.nih.gov/pubmed/33915920
http://dx.doi.org/10.3390/jdb9020013