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Electroporation of Mouse Follicles, Oocytes and Embryos without Manipulating Zona Pellucida
Electroporation is an effective technique of transfection, but its efficiency depends on the optimization of various parameters. In this study, a simplified and efficient method of gene manipulation was standardized through electroporation to introduce a recombinant green fluorescent protein (GFP) c...
| Autores principales: | , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
MDPI
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167572/ https://www.ncbi.nlm.nih.gov/pubmed/33915920 http://dx.doi.org/10.3390/jdb9020013 |