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CDX2 regulates interleukin‐33 gene expression in intestinal epithelial cells (LS174T)
Dysregulation of interleukin‐33 (IL‐33) has been implicated in the pathogenesis of several autoimmune and inflammatory diseases, but few studies have examined transcriptional regulation of the IL33 gene. In the intestines, gene regulation is controlled by a transcription factor network of which the...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167865/ https://www.ncbi.nlm.nih.gov/pubmed/33838073 http://dx.doi.org/10.1002/2211-5463.13161 |
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author | Larsen, Sylvester Seidelin, Jakob Benedict Davidsen, Johanne Dahlgaard, Katja Nielsen, Claus Henrik Bennett, Eric Paul Pedersen, Ole Birger Coskun, Mehmet Troelsen, Jesper Thorvald |
author_facet | Larsen, Sylvester Seidelin, Jakob Benedict Davidsen, Johanne Dahlgaard, Katja Nielsen, Claus Henrik Bennett, Eric Paul Pedersen, Ole Birger Coskun, Mehmet Troelsen, Jesper Thorvald |
author_sort | Larsen, Sylvester |
collection | PubMed |
description | Dysregulation of interleukin‐33 (IL‐33) has been implicated in the pathogenesis of several autoimmune and inflammatory diseases, but few studies have examined transcriptional regulation of the IL33 gene. In the intestines, gene regulation is controlled by a transcription factor network of which the intestinal‐specific transcription factor CDX2 is a key component. In this study, we investigated whether CDX2 regulates IL33 mRNA expression. We examined IL33 mRNA expression in primary colonic epithelial cells from healthy humans and epithelial cell lines, revealing high expression levels in primary colonic and LS174T cells. Combining genomics data (ChIP‐seq, RNA‐seq) and IL33 promoter analyses in LS174T cells revealed intronic enhancer activity in the IL33 gene that is dependent on CDX2 expression. Western blotting and qRT‐PCR confirmed that IL33 expression is upregulated in a CDX2 concentration‐dependent manner, thereby providing the first evidence that CDX2 regulates the expression of IL33. |
format | Online Article Text |
id | pubmed-8167865 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-81678652021-06-05 CDX2 regulates interleukin‐33 gene expression in intestinal epithelial cells (LS174T) Larsen, Sylvester Seidelin, Jakob Benedict Davidsen, Johanne Dahlgaard, Katja Nielsen, Claus Henrik Bennett, Eric Paul Pedersen, Ole Birger Coskun, Mehmet Troelsen, Jesper Thorvald FEBS Open Bio Research Articles Dysregulation of interleukin‐33 (IL‐33) has been implicated in the pathogenesis of several autoimmune and inflammatory diseases, but few studies have examined transcriptional regulation of the IL33 gene. In the intestines, gene regulation is controlled by a transcription factor network of which the intestinal‐specific transcription factor CDX2 is a key component. In this study, we investigated whether CDX2 regulates IL33 mRNA expression. We examined IL33 mRNA expression in primary colonic epithelial cells from healthy humans and epithelial cell lines, revealing high expression levels in primary colonic and LS174T cells. Combining genomics data (ChIP‐seq, RNA‐seq) and IL33 promoter analyses in LS174T cells revealed intronic enhancer activity in the IL33 gene that is dependent on CDX2 expression. Western blotting and qRT‐PCR confirmed that IL33 expression is upregulated in a CDX2 concentration‐dependent manner, thereby providing the first evidence that CDX2 regulates the expression of IL33. John Wiley and Sons Inc. 2021-05-02 /pmc/articles/PMC8167865/ /pubmed/33838073 http://dx.doi.org/10.1002/2211-5463.13161 Text en © 2021 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Larsen, Sylvester Seidelin, Jakob Benedict Davidsen, Johanne Dahlgaard, Katja Nielsen, Claus Henrik Bennett, Eric Paul Pedersen, Ole Birger Coskun, Mehmet Troelsen, Jesper Thorvald CDX2 regulates interleukin‐33 gene expression in intestinal epithelial cells (LS174T) |
title | CDX2 regulates interleukin‐33 gene expression in intestinal epithelial cells (LS174T) |
title_full | CDX2 regulates interleukin‐33 gene expression in intestinal epithelial cells (LS174T) |
title_fullStr | CDX2 regulates interleukin‐33 gene expression in intestinal epithelial cells (LS174T) |
title_full_unstemmed | CDX2 regulates interleukin‐33 gene expression in intestinal epithelial cells (LS174T) |
title_short | CDX2 regulates interleukin‐33 gene expression in intestinal epithelial cells (LS174T) |
title_sort | cdx2 regulates interleukin‐33 gene expression in intestinal epithelial cells (ls174t) |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167865/ https://www.ncbi.nlm.nih.gov/pubmed/33838073 http://dx.doi.org/10.1002/2211-5463.13161 |
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