Cargando…
Myeloid-derived growth factor regulates neutrophil motility in interstitial tissue damage
Neutrophil recruitment to tissue damage is essential for host defense but can also impede tissue repair. The cues that differentially regulate neutrophil responses to tissue damage and infection remain unclear. Here, we report that the paracrine factor myeloid-derived growth factor (MYDGF) is induce...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Rockefeller University Press
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167897/ https://www.ncbi.nlm.nih.gov/pubmed/34047769 http://dx.doi.org/10.1083/jcb.202103054 |
_version_ | 1783701782005809152 |
---|---|
author | Houseright, Ruth A. Miskolci, Veronika Mulvaney, Oscar Bortnov, Valeriu Mosher, Deane F. Rindy, Julie Bennin, David A. Huttenlocher, Anna |
author_facet | Houseright, Ruth A. Miskolci, Veronika Mulvaney, Oscar Bortnov, Valeriu Mosher, Deane F. Rindy, Julie Bennin, David A. Huttenlocher, Anna |
author_sort | Houseright, Ruth A. |
collection | PubMed |
description | Neutrophil recruitment to tissue damage is essential for host defense but can also impede tissue repair. The cues that differentially regulate neutrophil responses to tissue damage and infection remain unclear. Here, we report that the paracrine factor myeloid-derived growth factor (MYDGF) is induced by tissue damage and regulates neutrophil motility to damaged, but not infected, tissues in zebrafish larvae. Depletion of MYDGF impairs wound healing, and this phenotype is rescued by depleting neutrophils. Live imaging and photoconversion reveal impaired neutrophil reverse migration and inflammation resolution in mydgf mutants. We found that persistent neutrophil inflammation in tissues of mydgf mutants was dependent on the HIF-1α pathway. Taken together, our data suggest that MYDGF is a damage signal that regulates neutrophil interstitial motility and inflammation through a HIF-1α pathway in response to tissue damage. |
format | Online Article Text |
id | pubmed-8167897 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-81678972022-02-02 Myeloid-derived growth factor regulates neutrophil motility in interstitial tissue damage Houseright, Ruth A. Miskolci, Veronika Mulvaney, Oscar Bortnov, Valeriu Mosher, Deane F. Rindy, Julie Bennin, David A. Huttenlocher, Anna J Cell Biol Report Neutrophil recruitment to tissue damage is essential for host defense but can also impede tissue repair. The cues that differentially regulate neutrophil responses to tissue damage and infection remain unclear. Here, we report that the paracrine factor myeloid-derived growth factor (MYDGF) is induced by tissue damage and regulates neutrophil motility to damaged, but not infected, tissues in zebrafish larvae. Depletion of MYDGF impairs wound healing, and this phenotype is rescued by depleting neutrophils. Live imaging and photoconversion reveal impaired neutrophil reverse migration and inflammation resolution in mydgf mutants. We found that persistent neutrophil inflammation in tissues of mydgf mutants was dependent on the HIF-1α pathway. Taken together, our data suggest that MYDGF is a damage signal that regulates neutrophil interstitial motility and inflammation through a HIF-1α pathway in response to tissue damage. Rockefeller University Press 2021-05-28 /pmc/articles/PMC8167897/ /pubmed/34047769 http://dx.doi.org/10.1083/jcb.202103054 Text en © 2021 Houseright et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Report Houseright, Ruth A. Miskolci, Veronika Mulvaney, Oscar Bortnov, Valeriu Mosher, Deane F. Rindy, Julie Bennin, David A. Huttenlocher, Anna Myeloid-derived growth factor regulates neutrophil motility in interstitial tissue damage |
title | Myeloid-derived growth factor regulates neutrophil motility in interstitial tissue damage |
title_full | Myeloid-derived growth factor regulates neutrophil motility in interstitial tissue damage |
title_fullStr | Myeloid-derived growth factor regulates neutrophil motility in interstitial tissue damage |
title_full_unstemmed | Myeloid-derived growth factor regulates neutrophil motility in interstitial tissue damage |
title_short | Myeloid-derived growth factor regulates neutrophil motility in interstitial tissue damage |
title_sort | myeloid-derived growth factor regulates neutrophil motility in interstitial tissue damage |
topic | Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167897/ https://www.ncbi.nlm.nih.gov/pubmed/34047769 http://dx.doi.org/10.1083/jcb.202103054 |
work_keys_str_mv | AT houserightrutha myeloidderivedgrowthfactorregulatesneutrophilmotilityininterstitialtissuedamage AT miskolciveronika myeloidderivedgrowthfactorregulatesneutrophilmotilityininterstitialtissuedamage AT mulvaneyoscar myeloidderivedgrowthfactorregulatesneutrophilmotilityininterstitialtissuedamage AT bortnovvaleriu myeloidderivedgrowthfactorregulatesneutrophilmotilityininterstitialtissuedamage AT mosherdeanef myeloidderivedgrowthfactorregulatesneutrophilmotilityininterstitialtissuedamage AT rindyjulie myeloidderivedgrowthfactorregulatesneutrophilmotilityininterstitialtissuedamage AT bennindavida myeloidderivedgrowthfactorregulatesneutrophilmotilityininterstitialtissuedamage AT huttenlocheranna myeloidderivedgrowthfactorregulatesneutrophilmotilityininterstitialtissuedamage |