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Electrophysiological properties of maxillary trigeminal Aβ-afferent neurons of rats

Aβ-afferents in maxillary or V2 trigeminal ganglion (TG) neurons are somatosensory neurons that may be involved in both non-nociceptive and nociceptive functions in orofacial regions. However, electrophysiological properties of these V2 trigeminal Aβ-afferent neurons have not been well characterized...

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Autores principales: Okutsu, Yuya, Yamada, Akihiro, Tonomura, Sotatsu, Vaden, Ryan J, Gu, Jianguo G
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8168172/
https://www.ncbi.nlm.nih.gov/pubmed/34056968
http://dx.doi.org/10.1177/17448069211021271
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author Okutsu, Yuya
Yamada, Akihiro
Tonomura, Sotatsu
Vaden, Ryan J
Gu, Jianguo G
author_facet Okutsu, Yuya
Yamada, Akihiro
Tonomura, Sotatsu
Vaden, Ryan J
Gu, Jianguo G
author_sort Okutsu, Yuya
collection PubMed
description Aβ-afferents in maxillary or V2 trigeminal ganglion (TG) neurons are somatosensory neurons that may be involved in both non-nociceptive and nociceptive functions in orofacial regions. However, electrophysiological properties of these V2 trigeminal Aβ-afferent neurons have not been well characterized so far. Here, we used rat ex vivo trigeminal nerve preparations and applied patch-clamp recordings to large-sized V2 TG neurons to characterize their electrophysiological properties. All the cells recorded had afferent conduction velocities in the range of Aβ-afferent conduction speeds. However, these V2 trigeminal Aβ-afferent neurons displayed different action potential (AP) properties. APs showed fast kinetics in some cells but slow kinetics with shoulders in repolarization phases in other cells. Based on the derivatives of voltages in AP repolarization with time (dV/dt), we classified V2 trigeminal Aβ-afferent neurons into four types: type I, type II, type IIIa and type IIIb. Type I V2 trigeminal Aβ-afferent neurons had the largest dV/dt of repolarization, the fastest AP conduction velocities, the shortest AP and afterhyperpolarization (AHP) durations, and the highest AP success rates. In contrast, type IIIb V2 trigeminal Aβ-afferent neurons had the smallest dV/dt of AP repolarization, the slowest AP conduction velocities, the longest AP and AHP durations, and the lowest AP success rates. The type IIIb cells also had significantly lower voltage-activated K(+) currents. For type II and type IIIa V2 trigeminal Aβ-afferent neurons, AP parameters were in the range between those of type I and type IIIb V2 trigeminal Aβ-afferent neurons. Our electrophysiological classification of V2 trigeminal Aβ-afferent neurons may be useful in future to study their non-nociceptive and nociceptive functions in orofacial regions.
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spelling pubmed-81681722021-06-07 Electrophysiological properties of maxillary trigeminal Aβ-afferent neurons of rats Okutsu, Yuya Yamada, Akihiro Tonomura, Sotatsu Vaden, Ryan J Gu, Jianguo G Mol Pain Research Article Aβ-afferents in maxillary or V2 trigeminal ganglion (TG) neurons are somatosensory neurons that may be involved in both non-nociceptive and nociceptive functions in orofacial regions. However, electrophysiological properties of these V2 trigeminal Aβ-afferent neurons have not been well characterized so far. Here, we used rat ex vivo trigeminal nerve preparations and applied patch-clamp recordings to large-sized V2 TG neurons to characterize their electrophysiological properties. All the cells recorded had afferent conduction velocities in the range of Aβ-afferent conduction speeds. However, these V2 trigeminal Aβ-afferent neurons displayed different action potential (AP) properties. APs showed fast kinetics in some cells but slow kinetics with shoulders in repolarization phases in other cells. Based on the derivatives of voltages in AP repolarization with time (dV/dt), we classified V2 trigeminal Aβ-afferent neurons into four types: type I, type II, type IIIa and type IIIb. Type I V2 trigeminal Aβ-afferent neurons had the largest dV/dt of repolarization, the fastest AP conduction velocities, the shortest AP and afterhyperpolarization (AHP) durations, and the highest AP success rates. In contrast, type IIIb V2 trigeminal Aβ-afferent neurons had the smallest dV/dt of AP repolarization, the slowest AP conduction velocities, the longest AP and AHP durations, and the lowest AP success rates. The type IIIb cells also had significantly lower voltage-activated K(+) currents. For type II and type IIIa V2 trigeminal Aβ-afferent neurons, AP parameters were in the range between those of type I and type IIIb V2 trigeminal Aβ-afferent neurons. Our electrophysiological classification of V2 trigeminal Aβ-afferent neurons may be useful in future to study their non-nociceptive and nociceptive functions in orofacial regions. SAGE Publications 2021-05-30 /pmc/articles/PMC8168172/ /pubmed/34056968 http://dx.doi.org/10.1177/17448069211021271 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Research Article
Okutsu, Yuya
Yamada, Akihiro
Tonomura, Sotatsu
Vaden, Ryan J
Gu, Jianguo G
Electrophysiological properties of maxillary trigeminal Aβ-afferent neurons of rats
title Electrophysiological properties of maxillary trigeminal Aβ-afferent neurons of rats
title_full Electrophysiological properties of maxillary trigeminal Aβ-afferent neurons of rats
title_fullStr Electrophysiological properties of maxillary trigeminal Aβ-afferent neurons of rats
title_full_unstemmed Electrophysiological properties of maxillary trigeminal Aβ-afferent neurons of rats
title_short Electrophysiological properties of maxillary trigeminal Aβ-afferent neurons of rats
title_sort electrophysiological properties of maxillary trigeminal aβ-afferent neurons of rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8168172/
https://www.ncbi.nlm.nih.gov/pubmed/34056968
http://dx.doi.org/10.1177/17448069211021271
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