Unified platform for genetic and serological detection of COVID-19 with single-molecule technology

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The COVID-19 pandemic raises the need for diverse diagnostic approaches to rapidly detect different stages of viral infection. The flexible and quantitative nature of single-molecule imaging technology renders it optimal for development of new diagnostic tools. Here we present a proof-of-concept for...

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Autores principales: Furth, Noa, Shilo, Shay, Cohen, Niv, Erez, Nir, Fedyuk, Vadim, Schrager, Alexander M., Weinberger, Adina, Dror, Amiel A., Zigron, Asaf, Shehadeh, Mona, Sela, Eyal, Srouji, Samer, Amit, Sharon, Levy, Itzchak, Segal, Eran, Dahan, Rony, Jones, Dan, Douek, Daniel C., Shema, Efrat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8168389/
https://www.ncbi.nlm.nih.gov/pubmed/34075385
http://dx.doi.org/10.1101/2021.05.25.21257501
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author Furth, Noa
Shilo, Shay
Cohen, Niv
Erez, Nir
Fedyuk, Vadim
Schrager, Alexander M.
Weinberger, Adina
Dror, Amiel A.
Zigron, Asaf
Shehadeh, Mona
Sela, Eyal
Srouji, Samer
Amit, Sharon
Levy, Itzchak
Segal, Eran
Dahan, Rony
Jones, Dan
Douek, Daniel C.
Shema, Efrat
author_facet Furth, Noa
Shilo, Shay
Cohen, Niv
Erez, Nir
Fedyuk, Vadim
Schrager, Alexander M.
Weinberger, Adina
Dror, Amiel A.
Zigron, Asaf
Shehadeh, Mona
Sela, Eyal
Srouji, Samer
Amit, Sharon
Levy, Itzchak
Segal, Eran
Dahan, Rony
Jones, Dan
Douek, Daniel C.
Shema, Efrat
author_sort Furth, Noa
collection PubMed
description The COVID-19 pandemic raises the need for diverse diagnostic approaches to rapidly detect different stages of viral infection. The flexible and quantitative nature of single-molecule imaging technology renders it optimal for development of new diagnostic tools. Here we present a proof-of-concept for a single-molecule based, enzyme-free assay for detection of SARS-CoV-2. The unified platform we developed allows direct detection of the viral genetic material from patients’ samples, as well as their immune response consisting of IgG and IgM antibodies. Thus, it establishes a platform for diagnostics of COVID-19, which could also be adjusted to diagnose additional pathogens.
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spelling pubmed-81683892021-06-02 Unified platform for genetic and serological detection of COVID-19 with single-molecule technology Furth, Noa Shilo, Shay Cohen, Niv Erez, Nir Fedyuk, Vadim Schrager, Alexander M. Weinberger, Adina Dror, Amiel A. Zigron, Asaf Shehadeh, Mona Sela, Eyal Srouji, Samer Amit, Sharon Levy, Itzchak Segal, Eran Dahan, Rony Jones, Dan Douek, Daniel C. Shema, Efrat medRxiv Article The COVID-19 pandemic raises the need for diverse diagnostic approaches to rapidly detect different stages of viral infection. The flexible and quantitative nature of single-molecule imaging technology renders it optimal for development of new diagnostic tools. Here we present a proof-of-concept for a single-molecule based, enzyme-free assay for detection of SARS-CoV-2. The unified platform we developed allows direct detection of the viral genetic material from patients’ samples, as well as their immune response consisting of IgG and IgM antibodies. Thus, it establishes a platform for diagnostics of COVID-19, which could also be adjusted to diagnose additional pathogens. Cold Spring Harbor Laboratory 2021-07-27 /pmc/articles/PMC8168389/ /pubmed/34075385 http://dx.doi.org/10.1101/2021.05.25.21257501 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator.
spellingShingle Article
Furth, Noa
Shilo, Shay
Cohen, Niv
Erez, Nir
Fedyuk, Vadim
Schrager, Alexander M.
Weinberger, Adina
Dror, Amiel A.
Zigron, Asaf
Shehadeh, Mona
Sela, Eyal
Srouji, Samer
Amit, Sharon
Levy, Itzchak
Segal, Eran
Dahan, Rony
Jones, Dan
Douek, Daniel C.
Shema, Efrat
Unified platform for genetic and serological detection of COVID-19 with single-molecule technology
title Unified platform for genetic and serological detection of COVID-19 with single-molecule technology
title_full Unified platform for genetic and serological detection of COVID-19 with single-molecule technology
title_fullStr Unified platform for genetic and serological detection of COVID-19 with single-molecule technology
title_full_unstemmed Unified platform for genetic and serological detection of COVID-19 with single-molecule technology
title_short Unified platform for genetic and serological detection of COVID-19 with single-molecule technology
title_sort unified platform for genetic and serological detection of covid-19 with single-molecule technology
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8168389/
https://www.ncbi.nlm.nih.gov/pubmed/34075385
http://dx.doi.org/10.1101/2021.05.25.21257501
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