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Mutual chemical effect of autograft and octacalcium phosphate implantation on enhancing intramembranous bone regeneration

This study examined the effect of a mixture of octacalcium phosphate (OCP) and autologous bone on bone regeneration in rat calvaria critical-sized defect (CSD). Mechanically mixed OCP and autologous bone granules (OCP+Auto), approximately 500 to 1000 μm in diameter, and each individual material were...

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Autores principales: Ozaki, Hisashi, Hamai, Ryo, Shiwaku, Yukari, Sakai, Susumu, Tsuchiya, Kaori, Suzuki, Osamu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8168741/
https://www.ncbi.nlm.nih.gov/pubmed/34104115
http://dx.doi.org/10.1080/14686996.2021.1916378
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author Ozaki, Hisashi
Hamai, Ryo
Shiwaku, Yukari
Sakai, Susumu
Tsuchiya, Kaori
Suzuki, Osamu
author_facet Ozaki, Hisashi
Hamai, Ryo
Shiwaku, Yukari
Sakai, Susumu
Tsuchiya, Kaori
Suzuki, Osamu
author_sort Ozaki, Hisashi
collection PubMed
description This study examined the effect of a mixture of octacalcium phosphate (OCP) and autologous bone on bone regeneration in rat calvaria critical-sized defect (CSD). Mechanically mixed OCP and autologous bone granules (OCP+Auto), approximately 500 to 1000 μm in diameter, and each individual material were implanted in rat CSD for 8 weeks, and subjected to X-ray micro-computed tomography (micro-CT), histology, tartrate-resistant acid phosphatase (TRAP) staining, and histomorphometry for bone regeneration. Osteoblastic differentiation from mesenchymal stem cells (D1 cells) was examined in the presence of non-contacting materials by alkaline phosphatase (ALP) activity for 21 days. The material properties and medium composition before and after the incubation were determined by selected area electron diffraction (SAED) under transmission electron microscopy (TEM), Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), and chemical analysis. The results showed that while bone formation coupled with TRAP-positive osteoclastic resorption and cellular ALP activity were the highest in the Auto group, a positive effect per OCP weight or per autologous bone weight on ALP activity was found. Although the OCP structure was maintained even after the incubation (SAED), micro-deposits were grown on OCP surfaces (TEM). Fibrous tissue was also exposed on the autologous bone surfaces (SEM). Through FT-IR absorption, it was determined that bone mineral-like characteristics of the phosphate group increased in the OCP + Auto group. These findings were interpreted as a structural change from OCP to the apatitic phase, a conclusion supported by the medium degree of saturation changes. The results demonstrate the mutual chemical effect of mixing OCP with autologous bone as an active bone substitute material.
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spelling pubmed-81687412021-06-07 Mutual chemical effect of autograft and octacalcium phosphate implantation on enhancing intramembranous bone regeneration Ozaki, Hisashi Hamai, Ryo Shiwaku, Yukari Sakai, Susumu Tsuchiya, Kaori Suzuki, Osamu Sci Technol Adv Mater Focus on Trends in Biomaterials in Japan This study examined the effect of a mixture of octacalcium phosphate (OCP) and autologous bone on bone regeneration in rat calvaria critical-sized defect (CSD). Mechanically mixed OCP and autologous bone granules (OCP+Auto), approximately 500 to 1000 μm in diameter, and each individual material were implanted in rat CSD for 8 weeks, and subjected to X-ray micro-computed tomography (micro-CT), histology, tartrate-resistant acid phosphatase (TRAP) staining, and histomorphometry for bone regeneration. Osteoblastic differentiation from mesenchymal stem cells (D1 cells) was examined in the presence of non-contacting materials by alkaline phosphatase (ALP) activity for 21 days. The material properties and medium composition before and after the incubation were determined by selected area electron diffraction (SAED) under transmission electron microscopy (TEM), Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), and chemical analysis. The results showed that while bone formation coupled with TRAP-positive osteoclastic resorption and cellular ALP activity were the highest in the Auto group, a positive effect per OCP weight or per autologous bone weight on ALP activity was found. Although the OCP structure was maintained even after the incubation (SAED), micro-deposits were grown on OCP surfaces (TEM). Fibrous tissue was also exposed on the autologous bone surfaces (SEM). Through FT-IR absorption, it was determined that bone mineral-like characteristics of the phosphate group increased in the OCP + Auto group. These findings were interpreted as a structural change from OCP to the apatitic phase, a conclusion supported by the medium degree of saturation changes. The results demonstrate the mutual chemical effect of mixing OCP with autologous bone as an active bone substitute material. Taylor & Francis 2021-05-28 /pmc/articles/PMC8168741/ /pubmed/34104115 http://dx.doi.org/10.1080/14686996.2021.1916378 Text en © 2021 The Author(s). Published by National Institute for Materials Science in partnership with Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Focus on Trends in Biomaterials in Japan
Ozaki, Hisashi
Hamai, Ryo
Shiwaku, Yukari
Sakai, Susumu
Tsuchiya, Kaori
Suzuki, Osamu
Mutual chemical effect of autograft and octacalcium phosphate implantation on enhancing intramembranous bone regeneration
title Mutual chemical effect of autograft and octacalcium phosphate implantation on enhancing intramembranous bone regeneration
title_full Mutual chemical effect of autograft and octacalcium phosphate implantation on enhancing intramembranous bone regeneration
title_fullStr Mutual chemical effect of autograft and octacalcium phosphate implantation on enhancing intramembranous bone regeneration
title_full_unstemmed Mutual chemical effect of autograft and octacalcium phosphate implantation on enhancing intramembranous bone regeneration
title_short Mutual chemical effect of autograft and octacalcium phosphate implantation on enhancing intramembranous bone regeneration
title_sort mutual chemical effect of autograft and octacalcium phosphate implantation on enhancing intramembranous bone regeneration
topic Focus on Trends in Biomaterials in Japan
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8168741/
https://www.ncbi.nlm.nih.gov/pubmed/34104115
http://dx.doi.org/10.1080/14686996.2021.1916378
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