Responses of canine periodontal ligament cells to bubaline blood derived platelet rich fibrin in vitro

Platelet-rich fibrin (PRF) promotes wound healing by providing the release of growth factors. Here, the influence of Thai and Murrah bubaline blood derived PRF on canine periodontal ligament cells (cPDLs) was investigated. PRF was prepared from Thai and Murrah buffaloes with single centrifugation. R...

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Autores principales: Banyatworakul, Poranee, Osathanon, Thanaphum, Chumprasert, Sujin, Pavasant, Prasit, Pirarat, Nopadon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8169705/
https://www.ncbi.nlm.nih.gov/pubmed/34075114
http://dx.doi.org/10.1038/s41598-021-90906-z
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author Banyatworakul, Poranee
Osathanon, Thanaphum
Chumprasert, Sujin
Pavasant, Prasit
Pirarat, Nopadon
author_facet Banyatworakul, Poranee
Osathanon, Thanaphum
Chumprasert, Sujin
Pavasant, Prasit
Pirarat, Nopadon
author_sort Banyatworakul, Poranee
collection PubMed
description Platelet-rich fibrin (PRF) promotes wound healing by providing the release of growth factors. Here, the influence of Thai and Murrah bubaline blood derived PRF on canine periodontal ligament cells (cPDLs) was investigated. PRF was prepared from Thai and Murrah buffaloes with single centrifugation. Results demonstrated that Thai bubaline blood derived PRF exhibited fiber-mesh like morphology and contained more platelet entrapment than Murrah bubaline blood derived PRF. Both bubaline PRFs were able to degrade in vitro under condition with trypsin. Thai but not Murrah bubaline blood derived PRF promoted cPDLs proliferation in serum free and 2% serum culture conditions. Correspondingly, the significant upregulation of KI67 mRNA expression was observed in those cells treated with Thai bubaline blood derived PRF. However, both Thai and Murrah bubaline blood derived PRF accelerated cell migration in an in vitro wound healing assay and facilitated cell spreading. Further, cPDLs cultured in osteogenic induction medium supplemented with Thai bubaline blood derived PRF exhibited the increased mineral deposition in vitro. Frozen Thai bubaline blood derived PRF also promoted cell proliferation, KI67 mRNA expression, cell migration, and cell spreading in cPDLs. Taken these evidence together, bubaline blood derived PRF could provide potential benefits for canine periodontal tissue healing.
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spelling pubmed-81697052021-06-02 Responses of canine periodontal ligament cells to bubaline blood derived platelet rich fibrin in vitro Banyatworakul, Poranee Osathanon, Thanaphum Chumprasert, Sujin Pavasant, Prasit Pirarat, Nopadon Sci Rep Article Platelet-rich fibrin (PRF) promotes wound healing by providing the release of growth factors. Here, the influence of Thai and Murrah bubaline blood derived PRF on canine periodontal ligament cells (cPDLs) was investigated. PRF was prepared from Thai and Murrah buffaloes with single centrifugation. Results demonstrated that Thai bubaline blood derived PRF exhibited fiber-mesh like morphology and contained more platelet entrapment than Murrah bubaline blood derived PRF. Both bubaline PRFs were able to degrade in vitro under condition with trypsin. Thai but not Murrah bubaline blood derived PRF promoted cPDLs proliferation in serum free and 2% serum culture conditions. Correspondingly, the significant upregulation of KI67 mRNA expression was observed in those cells treated with Thai bubaline blood derived PRF. However, both Thai and Murrah bubaline blood derived PRF accelerated cell migration in an in vitro wound healing assay and facilitated cell spreading. Further, cPDLs cultured in osteogenic induction medium supplemented with Thai bubaline blood derived PRF exhibited the increased mineral deposition in vitro. Frozen Thai bubaline blood derived PRF also promoted cell proliferation, KI67 mRNA expression, cell migration, and cell spreading in cPDLs. Taken these evidence together, bubaline blood derived PRF could provide potential benefits for canine periodontal tissue healing. Nature Publishing Group UK 2021-06-01 /pmc/articles/PMC8169705/ /pubmed/34075114 http://dx.doi.org/10.1038/s41598-021-90906-z Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Banyatworakul, Poranee
Osathanon, Thanaphum
Chumprasert, Sujin
Pavasant, Prasit
Pirarat, Nopadon
Responses of canine periodontal ligament cells to bubaline blood derived platelet rich fibrin in vitro
title Responses of canine periodontal ligament cells to bubaline blood derived platelet rich fibrin in vitro
title_full Responses of canine periodontal ligament cells to bubaline blood derived platelet rich fibrin in vitro
title_fullStr Responses of canine periodontal ligament cells to bubaline blood derived platelet rich fibrin in vitro
title_full_unstemmed Responses of canine periodontal ligament cells to bubaline blood derived platelet rich fibrin in vitro
title_short Responses of canine periodontal ligament cells to bubaline blood derived platelet rich fibrin in vitro
title_sort responses of canine periodontal ligament cells to bubaline blood derived platelet rich fibrin in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8169705/
https://www.ncbi.nlm.nih.gov/pubmed/34075114
http://dx.doi.org/10.1038/s41598-021-90906-z
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