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In vitro plant regeneration system for date palm (Phoenix dactylifera L.): effect of chelated iron sources

BACKGROUND: Iron chelate sources and their concentrations are important factors in in vitro propagation of date palm. This study’s objective was to investigate the effect of the iron chelated form on the growth and development of tissue cultures of Barhee cultivar. RESULTS: The addition of FeEDDHA t...

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Autor principal: Al-Mayahi, Ahmed Madi Waheed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8169728/
https://www.ncbi.nlm.nih.gov/pubmed/34061279
http://dx.doi.org/10.1186/s43141-021-00177-4
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author Al-Mayahi, Ahmed Madi Waheed
author_facet Al-Mayahi, Ahmed Madi Waheed
author_sort Al-Mayahi, Ahmed Madi Waheed
collection PubMed
description BACKGROUND: Iron chelate sources and their concentrations are important factors in in vitro propagation of date palm. This study’s objective was to investigate the effect of the iron chelated form on the growth and development of tissue cultures of Barhee cultivar. RESULTS: The addition of FeEDDHA to the culture medium was more effective than FeEDTA on callus growth, shoot regeneration, and the number of shoots per jar, where the best result (220.8mg callus, 86.67% and 17.2 shoots per jar, respectively) was obtained by using 93.5 mg L(−1) FeEDDHA (5.6 mg L(−1) Fe), compared with other treatments. The results also indicate that using 93.5 mg L(−1) FeEDDHA (5.6 mg L(−1) Fe) as a supplement can decrease antioxidant enzymes CAT and POD activity compared to the rest of the treatments. Medium equipped with 187.0 mg L(−1) FeEDDHA (11.2 mg L(−1)Fe) had the highest rooting percentage and number of roots per shoot than other treatments. The biochemical analysis results showed that treatments with FeEDDHA of 280.5 mg L(−1) (16.8 mg L(−1) Fe) and 187.0 mg L(−1) (11.2 mg L(−1)Fe) significantly increased the iron content. The results showed that shoot maximum chlorophyll and endogenous IAA level content were recorded in a medium supplemented with 187.0 mg L(−1) FeEDDHA (11.2 mg L(−1)Fe) as Fe source. CONCLUSION: FeEDDHA used in the present study was proven to be a promising iron chelate source in comparison with the FeEDTA sources.
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spelling pubmed-81697282021-06-17 In vitro plant regeneration system for date palm (Phoenix dactylifera L.): effect of chelated iron sources Al-Mayahi, Ahmed Madi Waheed J Genet Eng Biotechnol Research BACKGROUND: Iron chelate sources and their concentrations are important factors in in vitro propagation of date palm. This study’s objective was to investigate the effect of the iron chelated form on the growth and development of tissue cultures of Barhee cultivar. RESULTS: The addition of FeEDDHA to the culture medium was more effective than FeEDTA on callus growth, shoot regeneration, and the number of shoots per jar, where the best result (220.8mg callus, 86.67% and 17.2 shoots per jar, respectively) was obtained by using 93.5 mg L(−1) FeEDDHA (5.6 mg L(−1) Fe), compared with other treatments. The results also indicate that using 93.5 mg L(−1) FeEDDHA (5.6 mg L(−1) Fe) as a supplement can decrease antioxidant enzymes CAT and POD activity compared to the rest of the treatments. Medium equipped with 187.0 mg L(−1) FeEDDHA (11.2 mg L(−1)Fe) had the highest rooting percentage and number of roots per shoot than other treatments. The biochemical analysis results showed that treatments with FeEDDHA of 280.5 mg L(−1) (16.8 mg L(−1) Fe) and 187.0 mg L(−1) (11.2 mg L(−1)Fe) significantly increased the iron content. The results showed that shoot maximum chlorophyll and endogenous IAA level content were recorded in a medium supplemented with 187.0 mg L(−1) FeEDDHA (11.2 mg L(−1)Fe) as Fe source. CONCLUSION: FeEDDHA used in the present study was proven to be a promising iron chelate source in comparison with the FeEDTA sources. Springer Berlin Heidelberg 2021-06-01 /pmc/articles/PMC8169728/ /pubmed/34061279 http://dx.doi.org/10.1186/s43141-021-00177-4 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research
Al-Mayahi, Ahmed Madi Waheed
In vitro plant regeneration system for date palm (Phoenix dactylifera L.): effect of chelated iron sources
title In vitro plant regeneration system for date palm (Phoenix dactylifera L.): effect of chelated iron sources
title_full In vitro plant regeneration system for date palm (Phoenix dactylifera L.): effect of chelated iron sources
title_fullStr In vitro plant regeneration system for date palm (Phoenix dactylifera L.): effect of chelated iron sources
title_full_unstemmed In vitro plant regeneration system for date palm (Phoenix dactylifera L.): effect of chelated iron sources
title_short In vitro plant regeneration system for date palm (Phoenix dactylifera L.): effect of chelated iron sources
title_sort in vitro plant regeneration system for date palm (phoenix dactylifera l.): effect of chelated iron sources
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8169728/
https://www.ncbi.nlm.nih.gov/pubmed/34061279
http://dx.doi.org/10.1186/s43141-021-00177-4
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