Cargando…
Genomic analysis and phylogenetic position of the complex IncC plasmid found in the Spanish monophasic clone of Salmonella enterica serovar Typhimurium
pUO-STmRV1 is an IncC plasmid discovered in the Spanish clone of the emergent monophasic variant of Salmonella enterica serovar Typhimurium, which has probably contributed to its epidemiological success. The sequence of the entire plasmid determined herein revealed a largely degenerated backbone wit...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8169936/ https://www.ncbi.nlm.nih.gov/pubmed/34075064 http://dx.doi.org/10.1038/s41598-021-90299-z |
_version_ | 1783702127740190720 |
---|---|
author | Vázquez, Xenia García, Patricia García, Vanesa de Toro, María Ladero, Víctor Heinisch, Jürgen J. Fernández, Javier Rodicio, Rosaura Rodicio, M. Rosario |
author_facet | Vázquez, Xenia García, Patricia García, Vanesa de Toro, María Ladero, Víctor Heinisch, Jürgen J. Fernández, Javier Rodicio, Rosaura Rodicio, M. Rosario |
author_sort | Vázquez, Xenia |
collection | PubMed |
description | pUO-STmRV1 is an IncC plasmid discovered in the Spanish clone of the emergent monophasic variant of Salmonella enterica serovar Typhimurium, which has probably contributed to its epidemiological success. The sequence of the entire plasmid determined herein revealed a largely degenerated backbone with accessory DNA incorporated at four different locations. The acquired DNA constitutes more than two-thirds of the pUO-STmRV1 genome and originates from plasmids of different incompatibility groups, including IncF (such as R100 and pSLT, the virulence plasmid specific of S. Typhimurium), IncN and IncI, from the integrative element GIsul2, or from yet unknown sources. In addition to pSLT virulence genes, the plasmid carries genes conferring resistance to widely-used antibiotics and heavy metals, together with a wealth of genetic elements involved in DNA mobility. The latter comprise class 1 integrons, transposons, pseudo-transposons, and insertion sequences, strikingly with 14 copies of IS26, which could have played a crucial role in the assembly of the complex plasmid. Typing of pUO-STmRV1 revealed backbone features characteristically associated with type 1 and type 2 IncC plasmids and could therefore be regarded as a hybrid plasmid. However, a rooted phylogenetic tree based on core genes indicates that it rather belongs to an ancient lineage which diverged at an early stage from the branch leading to most extant IncC plasmids detected so far. pUO-STmRV1 may have evolved at a time when uncontrolled use of antibiotics and biocides favored the accumulation of multiple resistance genes within an IncC backbone. The resulting plasmid thus allowed the Spanish clone to withstand a wide variety of adverse conditions, while simultaneously promoting its own propagation through vertical transmission. |
format | Online Article Text |
id | pubmed-8169936 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-81699362021-06-03 Genomic analysis and phylogenetic position of the complex IncC plasmid found in the Spanish monophasic clone of Salmonella enterica serovar Typhimurium Vázquez, Xenia García, Patricia García, Vanesa de Toro, María Ladero, Víctor Heinisch, Jürgen J. Fernández, Javier Rodicio, Rosaura Rodicio, M. Rosario Sci Rep Article pUO-STmRV1 is an IncC plasmid discovered in the Spanish clone of the emergent monophasic variant of Salmonella enterica serovar Typhimurium, which has probably contributed to its epidemiological success. The sequence of the entire plasmid determined herein revealed a largely degenerated backbone with accessory DNA incorporated at four different locations. The acquired DNA constitutes more than two-thirds of the pUO-STmRV1 genome and originates from plasmids of different incompatibility groups, including IncF (such as R100 and pSLT, the virulence plasmid specific of S. Typhimurium), IncN and IncI, from the integrative element GIsul2, or from yet unknown sources. In addition to pSLT virulence genes, the plasmid carries genes conferring resistance to widely-used antibiotics and heavy metals, together with a wealth of genetic elements involved in DNA mobility. The latter comprise class 1 integrons, transposons, pseudo-transposons, and insertion sequences, strikingly with 14 copies of IS26, which could have played a crucial role in the assembly of the complex plasmid. Typing of pUO-STmRV1 revealed backbone features characteristically associated with type 1 and type 2 IncC plasmids and could therefore be regarded as a hybrid plasmid. However, a rooted phylogenetic tree based on core genes indicates that it rather belongs to an ancient lineage which diverged at an early stage from the branch leading to most extant IncC plasmids detected so far. pUO-STmRV1 may have evolved at a time when uncontrolled use of antibiotics and biocides favored the accumulation of multiple resistance genes within an IncC backbone. The resulting plasmid thus allowed the Spanish clone to withstand a wide variety of adverse conditions, while simultaneously promoting its own propagation through vertical transmission. Nature Publishing Group UK 2021-06-01 /pmc/articles/PMC8169936/ /pubmed/34075064 http://dx.doi.org/10.1038/s41598-021-90299-z Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Vázquez, Xenia García, Patricia García, Vanesa de Toro, María Ladero, Víctor Heinisch, Jürgen J. Fernández, Javier Rodicio, Rosaura Rodicio, M. Rosario Genomic analysis and phylogenetic position of the complex IncC plasmid found in the Spanish monophasic clone of Salmonella enterica serovar Typhimurium |
title | Genomic analysis and phylogenetic position of the complex IncC plasmid found in the Spanish monophasic clone of Salmonella enterica serovar Typhimurium |
title_full | Genomic analysis and phylogenetic position of the complex IncC plasmid found in the Spanish monophasic clone of Salmonella enterica serovar Typhimurium |
title_fullStr | Genomic analysis and phylogenetic position of the complex IncC plasmid found in the Spanish monophasic clone of Salmonella enterica serovar Typhimurium |
title_full_unstemmed | Genomic analysis and phylogenetic position of the complex IncC plasmid found in the Spanish monophasic clone of Salmonella enterica serovar Typhimurium |
title_short | Genomic analysis and phylogenetic position of the complex IncC plasmid found in the Spanish monophasic clone of Salmonella enterica serovar Typhimurium |
title_sort | genomic analysis and phylogenetic position of the complex incc plasmid found in the spanish monophasic clone of salmonella enterica serovar typhimurium |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8169936/ https://www.ncbi.nlm.nih.gov/pubmed/34075064 http://dx.doi.org/10.1038/s41598-021-90299-z |
work_keys_str_mv | AT vazquezxenia genomicanalysisandphylogeneticpositionofthecomplexinccplasmidfoundinthespanishmonophasiccloneofsalmonellaentericaserovartyphimurium AT garciapatricia genomicanalysisandphylogeneticpositionofthecomplexinccplasmidfoundinthespanishmonophasiccloneofsalmonellaentericaserovartyphimurium AT garciavanesa genomicanalysisandphylogeneticpositionofthecomplexinccplasmidfoundinthespanishmonophasiccloneofsalmonellaentericaserovartyphimurium AT detoromaria genomicanalysisandphylogeneticpositionofthecomplexinccplasmidfoundinthespanishmonophasiccloneofsalmonellaentericaserovartyphimurium AT laderovictor genomicanalysisandphylogeneticpositionofthecomplexinccplasmidfoundinthespanishmonophasiccloneofsalmonellaentericaserovartyphimurium AT heinischjurgenj genomicanalysisandphylogeneticpositionofthecomplexinccplasmidfoundinthespanishmonophasiccloneofsalmonellaentericaserovartyphimurium AT fernandezjavier genomicanalysisandphylogeneticpositionofthecomplexinccplasmidfoundinthespanishmonophasiccloneofsalmonellaentericaserovartyphimurium AT rodiciorosaura genomicanalysisandphylogeneticpositionofthecomplexinccplasmidfoundinthespanishmonophasiccloneofsalmonellaentericaserovartyphimurium AT rodiciomrosario genomicanalysisandphylogeneticpositionofthecomplexinccplasmidfoundinthespanishmonophasiccloneofsalmonellaentericaserovartyphimurium |