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Biological assessment of a new ready-to-use hydraulic sealer

OBJECTIVES: This study compared the cytotoxicity, biocompatibility, and tenascin immunolabeling of a new ready-to-use hydraulic sealer (Bio-C Sealer) with MTA-Fillapex and white MTA-Angelus. MATERIALS AND METHODS: L929 fibroblasts were cultivated and exposed to undiluted and diluted material extract...

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Autores principales: Benetti, Francine, Gomes-Filho, João Eduardo, de Azevedo-Queiroz, India Olinta, Carminatti, Marina, Conti, Letícia Citelli, dos Reis-Prado, Alexandre Henrique, de Oliveira, Sandra Helena Penha, Ervolino, Edilson, Dezan-Júnior, Elói, Cintra, Luciano Tavares Angelo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Academy of Conservative Dentistry 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170373/
https://www.ncbi.nlm.nih.gov/pubmed/34123757
http://dx.doi.org/10.5395/rde.2021.46.e21
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author Benetti, Francine
Gomes-Filho, João Eduardo
de Azevedo-Queiroz, India Olinta
Carminatti, Marina
Conti, Letícia Citelli
dos Reis-Prado, Alexandre Henrique
de Oliveira, Sandra Helena Penha
Ervolino, Edilson
Dezan-Júnior, Elói
Cintra, Luciano Tavares Angelo
author_facet Benetti, Francine
Gomes-Filho, João Eduardo
de Azevedo-Queiroz, India Olinta
Carminatti, Marina
Conti, Letícia Citelli
dos Reis-Prado, Alexandre Henrique
de Oliveira, Sandra Helena Penha
Ervolino, Edilson
Dezan-Júnior, Elói
Cintra, Luciano Tavares Angelo
author_sort Benetti, Francine
collection PubMed
description OBJECTIVES: This study compared the cytotoxicity, biocompatibility, and tenascin immunolabeling of a new ready-to-use hydraulic sealer (Bio-C Sealer) with MTA-Fillapex and white MTA-Angelus. MATERIALS AND METHODS: L929 fibroblasts were cultivated and exposed to undiluted and diluted material extracts. Polyethylene tubes with or without (the control) the materials were implanted into the dorsa of rats. At 7 days and 30 days, the rats were euthanized, and the specimens were prepared for analysis; inflammation and immunolabeling were measured, and statistical analysis was performed (p < 0.05). RESULTS: MTA-Fillapex exhibited greater cytotoxicity than the other materials at all time points (p < 0.05). The undiluted Bio-C Sealer exhibited greater cytocompatibility at 6 and 48 hours than white MTA-Angelus, with higher cell viability than in the control (p < 0.05). White MTA-Angelus displayed higher cell viability than the control at 24 hours, and the one-half dilution displayed similar results at both 6 and 48 hours (p < 0.05). At 7 days and 30 days, the groups exhibited moderate inflammation with thick fibrous capsules and mild inflammation with thin fibrous capsules, respectively (p > 0.05). At 7 days, moderate to strong immunolabeling was observed (p > 0.05). After 30 days, the control and MTA-Fillapex groups exhibited strong immunolabeling, the white MTA-Angelus group exhibited moderate immunolabeling (p > 0.05), and the Bio-C Sealer group exhibited low-to-moderate immunolabeling, differing significantly from the control (p < 0.05). CONCLUSIONS: Bio-C Sealer and white MTA-Angelus exhibited greater cytocompatibility than MTA-Fillapex; all materials displayed adequate biocompatibility and induced tenascin immunolabeling.
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spelling pubmed-81703732021-06-11 Biological assessment of a new ready-to-use hydraulic sealer Benetti, Francine Gomes-Filho, João Eduardo de Azevedo-Queiroz, India Olinta Carminatti, Marina Conti, Letícia Citelli dos Reis-Prado, Alexandre Henrique de Oliveira, Sandra Helena Penha Ervolino, Edilson Dezan-Júnior, Elói Cintra, Luciano Tavares Angelo Restor Dent Endod Research Article OBJECTIVES: This study compared the cytotoxicity, biocompatibility, and tenascin immunolabeling of a new ready-to-use hydraulic sealer (Bio-C Sealer) with MTA-Fillapex and white MTA-Angelus. MATERIALS AND METHODS: L929 fibroblasts were cultivated and exposed to undiluted and diluted material extracts. Polyethylene tubes with or without (the control) the materials were implanted into the dorsa of rats. At 7 days and 30 days, the rats were euthanized, and the specimens were prepared for analysis; inflammation and immunolabeling were measured, and statistical analysis was performed (p < 0.05). RESULTS: MTA-Fillapex exhibited greater cytotoxicity than the other materials at all time points (p < 0.05). The undiluted Bio-C Sealer exhibited greater cytocompatibility at 6 and 48 hours than white MTA-Angelus, with higher cell viability than in the control (p < 0.05). White MTA-Angelus displayed higher cell viability than the control at 24 hours, and the one-half dilution displayed similar results at both 6 and 48 hours (p < 0.05). At 7 days and 30 days, the groups exhibited moderate inflammation with thick fibrous capsules and mild inflammation with thin fibrous capsules, respectively (p > 0.05). At 7 days, moderate to strong immunolabeling was observed (p > 0.05). After 30 days, the control and MTA-Fillapex groups exhibited strong immunolabeling, the white MTA-Angelus group exhibited moderate immunolabeling (p > 0.05), and the Bio-C Sealer group exhibited low-to-moderate immunolabeling, differing significantly from the control (p < 0.05). CONCLUSIONS: Bio-C Sealer and white MTA-Angelus exhibited greater cytocompatibility than MTA-Fillapex; all materials displayed adequate biocompatibility and induced tenascin immunolabeling. The Korean Academy of Conservative Dentistry 2021-03-24 /pmc/articles/PMC8170373/ /pubmed/34123757 http://dx.doi.org/10.5395/rde.2021.46.e21 Text en Copyright © 2021. The Korean Academy of Conservative Dentistry https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Benetti, Francine
Gomes-Filho, João Eduardo
de Azevedo-Queiroz, India Olinta
Carminatti, Marina
Conti, Letícia Citelli
dos Reis-Prado, Alexandre Henrique
de Oliveira, Sandra Helena Penha
Ervolino, Edilson
Dezan-Júnior, Elói
Cintra, Luciano Tavares Angelo
Biological assessment of a new ready-to-use hydraulic sealer
title Biological assessment of a new ready-to-use hydraulic sealer
title_full Biological assessment of a new ready-to-use hydraulic sealer
title_fullStr Biological assessment of a new ready-to-use hydraulic sealer
title_full_unstemmed Biological assessment of a new ready-to-use hydraulic sealer
title_short Biological assessment of a new ready-to-use hydraulic sealer
title_sort biological assessment of a new ready-to-use hydraulic sealer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170373/
https://www.ncbi.nlm.nih.gov/pubmed/34123757
http://dx.doi.org/10.5395/rde.2021.46.e21
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