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SDHB Suppresses the Tumorigenesis and Development of ccRCC by Inhibiting Glycolysis

Metabolic reprogramming is the prominent feature of clear cell renal cell carcinoma (ccRCC). Succinate dehydrogenase subunit B (SDHB) is one of subunits of mitochondrial respiratory chain complex II. The loss of SDHB function is closely related with metabolic changes in kidney cancer cells. However,...

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Detalles Bibliográficos
Autores principales: Fang, Zhiyu, Sun, Qiang, Yang, Huihui, Zheng, Junfang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170479/
https://www.ncbi.nlm.nih.gov/pubmed/34094922
http://dx.doi.org/10.3389/fonc.2021.639408
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author Fang, Zhiyu
Sun, Qiang
Yang, Huihui
Zheng, Junfang
author_facet Fang, Zhiyu
Sun, Qiang
Yang, Huihui
Zheng, Junfang
author_sort Fang, Zhiyu
collection PubMed
description Metabolic reprogramming is the prominent feature of clear cell renal cell carcinoma (ccRCC). Succinate dehydrogenase subunit B (SDHB) is one of subunits of mitochondrial respiratory chain complex II. The loss of SDHB function is closely related with metabolic changes in kidney cancer cells. However, the role and molecular mechanism of SDHB in ccRCC occurrence and progression are still unclear. In this study, the results of bioinformatics analyses on GEO, TCGA and oncomine databases and immunohistochemistry showed that the expression level of SDHB was downregulated in ccRCC tissues. SDHB level was gradually downregulated as ccRCC stage and grade progressed. The low level of SDHB was associated with poor prognosis of ccRCC patients, especially for advanced ccRCC patients. Increased methylation levels in SDHB gene promoter led to the downregulation of SDHB level in ccRCC tissues. SDHB was correlated with many metabolism related genes and its interacting proteins were enriched in metabolic pathways. SDHB overexpression suppressed the proliferation, colony formation and migration of ccRCC cells by inhibiting aerobic glycolysis. SDHB may be a potential prognostic marker and therapeutic target for ccRCC.
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spelling pubmed-81704792021-06-03 SDHB Suppresses the Tumorigenesis and Development of ccRCC by Inhibiting Glycolysis Fang, Zhiyu Sun, Qiang Yang, Huihui Zheng, Junfang Front Oncol Oncology Metabolic reprogramming is the prominent feature of clear cell renal cell carcinoma (ccRCC). Succinate dehydrogenase subunit B (SDHB) is one of subunits of mitochondrial respiratory chain complex II. The loss of SDHB function is closely related with metabolic changes in kidney cancer cells. However, the role and molecular mechanism of SDHB in ccRCC occurrence and progression are still unclear. In this study, the results of bioinformatics analyses on GEO, TCGA and oncomine databases and immunohistochemistry showed that the expression level of SDHB was downregulated in ccRCC tissues. SDHB level was gradually downregulated as ccRCC stage and grade progressed. The low level of SDHB was associated with poor prognosis of ccRCC patients, especially for advanced ccRCC patients. Increased methylation levels in SDHB gene promoter led to the downregulation of SDHB level in ccRCC tissues. SDHB was correlated with many metabolism related genes and its interacting proteins were enriched in metabolic pathways. SDHB overexpression suppressed the proliferation, colony formation and migration of ccRCC cells by inhibiting aerobic glycolysis. SDHB may be a potential prognostic marker and therapeutic target for ccRCC. Frontiers Media S.A. 2021-05-19 /pmc/articles/PMC8170479/ /pubmed/34094922 http://dx.doi.org/10.3389/fonc.2021.639408 Text en Copyright © 2021 Fang, Sun, Yang and Zheng https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Fang, Zhiyu
Sun, Qiang
Yang, Huihui
Zheng, Junfang
SDHB Suppresses the Tumorigenesis and Development of ccRCC by Inhibiting Glycolysis
title SDHB Suppresses the Tumorigenesis and Development of ccRCC by Inhibiting Glycolysis
title_full SDHB Suppresses the Tumorigenesis and Development of ccRCC by Inhibiting Glycolysis
title_fullStr SDHB Suppresses the Tumorigenesis and Development of ccRCC by Inhibiting Glycolysis
title_full_unstemmed SDHB Suppresses the Tumorigenesis and Development of ccRCC by Inhibiting Glycolysis
title_short SDHB Suppresses the Tumorigenesis and Development of ccRCC by Inhibiting Glycolysis
title_sort sdhb suppresses the tumorigenesis and development of ccrcc by inhibiting glycolysis
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170479/
https://www.ncbi.nlm.nih.gov/pubmed/34094922
http://dx.doi.org/10.3389/fonc.2021.639408
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