Endogenous localization of TOP-2 in C. elegans using a C-terminal GFP-tag

To investigate the dynamic localization of Topoisomerase II in live C. elegans we have generated a C-terminally GFP-tagged version of TOP-2 at the endogenous locus. We found that TOP-2::GFP localizes in a similar pattern to the previously published TOP-2::3XFLAG strain and does not disrupt the meiot...

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Detalles Bibliográficos
Autores principales: Rourke, Christine K., Murat, Darline, Hansen, Tyler J., Jaramillo-Lambert, Aimee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2021
Materias:
New Finding
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170510/
https://www.ncbi.nlm.nih.gov/pubmed/34095779
http://dx.doi.org/10.17912/micropub.biology.000402
Descripción
Sumario:To investigate the dynamic localization of Topoisomerase II in live C. elegans we have generated a C-terminally GFP-tagged version of TOP-2 at the endogenous locus. We found that TOP-2::GFP localizes in a similar pattern to the previously published TOP-2::3XFLAG strain and does not disrupt the meiotic chromosome segregation functions of this enzyme.

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