Endogenous localization of TOP-2 in C. elegans using a C-terminal GFP-tag

To investigate the dynamic localization of Topoisomerase II in live C. elegans we have generated a C-terminally GFP-tagged version of TOP-2 at the endogenous locus. We found that TOP-2::GFP localizes in a similar pattern to the previously published TOP-2::3XFLAG strain and does not disrupt the meiot...

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Detalles Bibliográficos
Autores principales: Rourke, Christine K., Murat, Darline, Hansen, Tyler J., Jaramillo-Lambert, Aimee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2021
Materias:
New Finding
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170510/
https://www.ncbi.nlm.nih.gov/pubmed/34095779
http://dx.doi.org/10.17912/micropub.biology.000402
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author Rourke, Christine K.
Murat, Darline
Hansen, Tyler J.
Jaramillo-Lambert, Aimee
author_facet Rourke, Christine K.
Murat, Darline
Hansen, Tyler J.
Jaramillo-Lambert, Aimee
author_sort Rourke, Christine K.
collection PubMed
description To investigate the dynamic localization of Topoisomerase II in live C. elegans we have generated a C-terminally GFP-tagged version of TOP-2 at the endogenous locus. We found that TOP-2::GFP localizes in a similar pattern to the previously published TOP-2::3XFLAG strain and does not disrupt the meiotic chromosome segregation functions of this enzyme.
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spelling pubmed-81705102021-06-04 Endogenous localization of TOP-2 in C. elegans using a C-terminal GFP-tag Rourke, Christine K. Murat, Darline Hansen, Tyler J. Jaramillo-Lambert, Aimee MicroPubl Biol New Finding To investigate the dynamic localization of Topoisomerase II in live C. elegans we have generated a C-terminally GFP-tagged version of TOP-2 at the endogenous locus. We found that TOP-2::GFP localizes in a similar pattern to the previously published TOP-2::3XFLAG strain and does not disrupt the meiotic chromosome segregation functions of this enzyme. Caltech Library 2021-05-28 /pmc/articles/PMC8170510/ /pubmed/34095779 http://dx.doi.org/10.17912/micropub.biology.000402 Text en Copyright: © 2021 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Finding
Rourke, Christine K.
Murat, Darline
Hansen, Tyler J.
Jaramillo-Lambert, Aimee
Endogenous localization of TOP-2 in C. elegans using a C-terminal GFP-tag
title Endogenous localization of TOP-2 in C. elegans using a C-terminal GFP-tag
title_full Endogenous localization of TOP-2 in C. elegans using a C-terminal GFP-tag
title_fullStr Endogenous localization of TOP-2 in C. elegans using a C-terminal GFP-tag
title_full_unstemmed Endogenous localization of TOP-2 in C. elegans using a C-terminal GFP-tag
title_short Endogenous localization of TOP-2 in C. elegans using a C-terminal GFP-tag
title_sort endogenous localization of top-2 in c. elegans using a c-terminal gfp-tag
topic New Finding
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170510/
https://www.ncbi.nlm.nih.gov/pubmed/34095779
http://dx.doi.org/10.17912/micropub.biology.000402
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