SARS-CoV-2 detection by a clinical diagnostic RT-LAMP assay

The ongoing pandemic of SARS-CoV-2 calls for rapid and cost-effective methods to accurately identify infected individuals. The vast majority of patient samples is assessed for viral RNA presence by RT-qPCR. Our biomedical research institute, in collaboration between partner hospitals and an accredit...

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Detalles Bibliográficos
Autores principales: Buck, Michael D., Poirier, Enzo Z., Cardoso, Ana, Frederico, Bruno, Canton, Johnathan, Barrell, Sam, Beale, Rupert, Byrne, Richard, Caidan, Simon, Crawford, Margaret, Cubitt, Laura, Gandhi, Sonia, Goldstone, Robert, Grant, Paul R., Gulati, Kiran, Hindmarsh, Steve, Howell, Michael, Hubank, Michael, Instrell, Rachael, Jiang, Ming, Kassiotis, George, Lu, Wei-Ting, MacRae, James I., Martini, Iana, Miller, Davin, Moore, David, Nastouli, Eleni, Nicod, Jerome, Nightingale, Luke, Olsen, Jessica, Oomatia, Amin, O'Reilly, Nicola, Rideg, Anett, Song, Ok-Ryul, Strange, Amy, Swanton, Charles, Turajlic, Samra, Wu, Mary, Reis e Sousa, Caetano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000 Research Limited 2021
Materias:
Method Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170534/
https://www.ncbi.nlm.nih.gov/pubmed/34095506
http://dx.doi.org/10.12688/wellcomeopenres.16517.2
Descripción
Sumario:The ongoing pandemic of SARS-CoV-2 calls for rapid and cost-effective methods to accurately identify infected individuals. The vast majority of patient samples is assessed for viral RNA presence by RT-qPCR. Our biomedical research institute, in collaboration between partner hospitals and an accredited clinical diagnostic laboratory, established a diagnostic testing pipeline that has reported on more than 252,000 RT-qPCR results since its commencement at the beginning of April 2020. However, due to ongoing demand and competition for critical resources, alternative testing strategies were sought. In this work, we present a clinically-validated procedure for high-throughput SARS-CoV-2 detection by RT-LAMP that is robust, reliable, repeatable, specific, and inexpensive.

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