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The effect of splicing MST1R in gastric cancer was enhanced by lncRNA FENDRR
Gastric cancer (GC) poses a serious threat to human health worldwide. Serine/arginine rich splicing factor 1 (SRSF1) has been reported to serve regulatory roles during the tumorigenesis of GC. In addition, the macrophage stimulating 1 receptor (MST1R) signaling pathway was found to participate in th...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170639/ https://www.ncbi.nlm.nih.gov/pubmed/34093754 http://dx.doi.org/10.3892/etm.2021.10230 |
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author | Zhou, Donghui Zhu, Xiaohua Wu, Xuan Zheng, Jingjing Tou, Laizhen Zhou, Yong |
author_facet | Zhou, Donghui Zhu, Xiaohua Wu, Xuan Zheng, Jingjing Tou, Laizhen Zhou, Yong |
author_sort | Zhou, Donghui |
collection | PubMed |
description | Gastric cancer (GC) poses a serious threat to human health worldwide. Serine/arginine rich splicing factor 1 (SRSF1) has been reported to serve regulatory roles during the tumorigenesis of GC. In addition, the macrophage stimulating 1 receptor (MST1R) signaling pathway was found to participate in the progression of GC. However, the association between MST1R and SRSF1 in the tumorigenesis of GC remains unclear. The expression levels of MST1R and the recepteur d'origine nantais (RON) Δ160 splicing variant were analyzed in cells using western blotting and immunofluorescence staining. Co-immunoprecipitation assays were used to investigate the interaction between SRSF1 and MST1R. A Cell Counting Kit-8 assay was performed to analyze cell viability. Flow cytometry and Transwell assays were used to determine cell apoptosis and invasiveness levels. The potential interaction between SFSR1 and long non-coding RNAs (lncRNAs) was investigated with an online bioinformatics tool. The findings of the present study revealed that the expression levels of MST1R and RON Δ160 were significantly upregulated in GC Kato III cells. SRSF1 was found to be regulated by the lncRNA FOXF1 adjacent non-coding developmental regulatory RNA (FENDRR). The knockdown of SRSF1 or FENDRR downregulated the expression levels of MST1R in Kato III cells. In addition, the expression levels of RON Δ160 were markedly downregulated in Kato III cells following the knockdown of FENDRR. Meanwhile, SRSF1 directly bound to MST1R, while this phenomenon was partially reversed by FENDRR short interfering RNA. FENDRR could interact with SRSF1 in Kato III cells and the knockdown of FENDRR also induced the apoptosis of GC cells. In conclusion, the findings of the present study suggested that the lncRNA FENDRR may function as an oncogene during the progression of GC by regulating alternative splicing of MST1R and SRSF1 expression levels. lncRNA FENDRR may serve as a potential marker for the diagnosis or target for the treatment of GC. |
format | Online Article Text |
id | pubmed-8170639 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-81706392021-06-04 The effect of splicing MST1R in gastric cancer was enhanced by lncRNA FENDRR Zhou, Donghui Zhu, Xiaohua Wu, Xuan Zheng, Jingjing Tou, Laizhen Zhou, Yong Exp Ther Med Articles Gastric cancer (GC) poses a serious threat to human health worldwide. Serine/arginine rich splicing factor 1 (SRSF1) has been reported to serve regulatory roles during the tumorigenesis of GC. In addition, the macrophage stimulating 1 receptor (MST1R) signaling pathway was found to participate in the progression of GC. However, the association between MST1R and SRSF1 in the tumorigenesis of GC remains unclear. The expression levels of MST1R and the recepteur d'origine nantais (RON) Δ160 splicing variant were analyzed in cells using western blotting and immunofluorescence staining. Co-immunoprecipitation assays were used to investigate the interaction between SRSF1 and MST1R. A Cell Counting Kit-8 assay was performed to analyze cell viability. Flow cytometry and Transwell assays were used to determine cell apoptosis and invasiveness levels. The potential interaction between SFSR1 and long non-coding RNAs (lncRNAs) was investigated with an online bioinformatics tool. The findings of the present study revealed that the expression levels of MST1R and RON Δ160 were significantly upregulated in GC Kato III cells. SRSF1 was found to be regulated by the lncRNA FOXF1 adjacent non-coding developmental regulatory RNA (FENDRR). The knockdown of SRSF1 or FENDRR downregulated the expression levels of MST1R in Kato III cells. In addition, the expression levels of RON Δ160 were markedly downregulated in Kato III cells following the knockdown of FENDRR. Meanwhile, SRSF1 directly bound to MST1R, while this phenomenon was partially reversed by FENDRR short interfering RNA. FENDRR could interact with SRSF1 in Kato III cells and the knockdown of FENDRR also induced the apoptosis of GC cells. In conclusion, the findings of the present study suggested that the lncRNA FENDRR may function as an oncogene during the progression of GC by regulating alternative splicing of MST1R and SRSF1 expression levels. lncRNA FENDRR may serve as a potential marker for the diagnosis or target for the treatment of GC. D.A. Spandidos 2021-08 2021-05-25 /pmc/articles/PMC8170639/ /pubmed/34093754 http://dx.doi.org/10.3892/etm.2021.10230 Text en Copyright: © Zhou et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Zhou, Donghui Zhu, Xiaohua Wu, Xuan Zheng, Jingjing Tou, Laizhen Zhou, Yong The effect of splicing MST1R in gastric cancer was enhanced by lncRNA FENDRR |
title | The effect of splicing MST1R in gastric cancer was enhanced by lncRNA FENDRR |
title_full | The effect of splicing MST1R in gastric cancer was enhanced by lncRNA FENDRR |
title_fullStr | The effect of splicing MST1R in gastric cancer was enhanced by lncRNA FENDRR |
title_full_unstemmed | The effect of splicing MST1R in gastric cancer was enhanced by lncRNA FENDRR |
title_short | The effect of splicing MST1R in gastric cancer was enhanced by lncRNA FENDRR |
title_sort | effect of splicing mst1r in gastric cancer was enhanced by lncrna fendrr |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170639/ https://www.ncbi.nlm.nih.gov/pubmed/34093754 http://dx.doi.org/10.3892/etm.2021.10230 |
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