Cargando…

The effect of splicing MST1R in gastric cancer was enhanced by lncRNA FENDRR

Gastric cancer (GC) poses a serious threat to human health worldwide. Serine/arginine rich splicing factor 1 (SRSF1) has been reported to serve regulatory roles during the tumorigenesis of GC. In addition, the macrophage stimulating 1 receptor (MST1R) signaling pathway was found to participate in th...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhou, Donghui, Zhu, Xiaohua, Wu, Xuan, Zheng, Jingjing, Tou, Laizhen, Zhou, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170639/
https://www.ncbi.nlm.nih.gov/pubmed/34093754
http://dx.doi.org/10.3892/etm.2021.10230
_version_ 1783702286333116416
author Zhou, Donghui
Zhu, Xiaohua
Wu, Xuan
Zheng, Jingjing
Tou, Laizhen
Zhou, Yong
author_facet Zhou, Donghui
Zhu, Xiaohua
Wu, Xuan
Zheng, Jingjing
Tou, Laizhen
Zhou, Yong
author_sort Zhou, Donghui
collection PubMed
description Gastric cancer (GC) poses a serious threat to human health worldwide. Serine/arginine rich splicing factor 1 (SRSF1) has been reported to serve regulatory roles during the tumorigenesis of GC. In addition, the macrophage stimulating 1 receptor (MST1R) signaling pathway was found to participate in the progression of GC. However, the association between MST1R and SRSF1 in the tumorigenesis of GC remains unclear. The expression levels of MST1R and the recepteur d'origine nantais (RON) Δ160 splicing variant were analyzed in cells using western blotting and immunofluorescence staining. Co-immunoprecipitation assays were used to investigate the interaction between SRSF1 and MST1R. A Cell Counting Kit-8 assay was performed to analyze cell viability. Flow cytometry and Transwell assays were used to determine cell apoptosis and invasiveness levels. The potential interaction between SFSR1 and long non-coding RNAs (lncRNAs) was investigated with an online bioinformatics tool. The findings of the present study revealed that the expression levels of MST1R and RON Δ160 were significantly upregulated in GC Kato III cells. SRSF1 was found to be regulated by the lncRNA FOXF1 adjacent non-coding developmental regulatory RNA (FENDRR). The knockdown of SRSF1 or FENDRR downregulated the expression levels of MST1R in Kato III cells. In addition, the expression levels of RON Δ160 were markedly downregulated in Kato III cells following the knockdown of FENDRR. Meanwhile, SRSF1 directly bound to MST1R, while this phenomenon was partially reversed by FENDRR short interfering RNA. FENDRR could interact with SRSF1 in Kato III cells and the knockdown of FENDRR also induced the apoptosis of GC cells. In conclusion, the findings of the present study suggested that the lncRNA FENDRR may function as an oncogene during the progression of GC by regulating alternative splicing of MST1R and SRSF1 expression levels. lncRNA FENDRR may serve as a potential marker for the diagnosis or target for the treatment of GC.
format Online
Article
Text
id pubmed-8170639
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-81706392021-06-04 The effect of splicing MST1R in gastric cancer was enhanced by lncRNA FENDRR Zhou, Donghui Zhu, Xiaohua Wu, Xuan Zheng, Jingjing Tou, Laizhen Zhou, Yong Exp Ther Med Articles Gastric cancer (GC) poses a serious threat to human health worldwide. Serine/arginine rich splicing factor 1 (SRSF1) has been reported to serve regulatory roles during the tumorigenesis of GC. In addition, the macrophage stimulating 1 receptor (MST1R) signaling pathway was found to participate in the progression of GC. However, the association between MST1R and SRSF1 in the tumorigenesis of GC remains unclear. The expression levels of MST1R and the recepteur d'origine nantais (RON) Δ160 splicing variant were analyzed in cells using western blotting and immunofluorescence staining. Co-immunoprecipitation assays were used to investigate the interaction between SRSF1 and MST1R. A Cell Counting Kit-8 assay was performed to analyze cell viability. Flow cytometry and Transwell assays were used to determine cell apoptosis and invasiveness levels. The potential interaction between SFSR1 and long non-coding RNAs (lncRNAs) was investigated with an online bioinformatics tool. The findings of the present study revealed that the expression levels of MST1R and RON Δ160 were significantly upregulated in GC Kato III cells. SRSF1 was found to be regulated by the lncRNA FOXF1 adjacent non-coding developmental regulatory RNA (FENDRR). The knockdown of SRSF1 or FENDRR downregulated the expression levels of MST1R in Kato III cells. In addition, the expression levels of RON Δ160 were markedly downregulated in Kato III cells following the knockdown of FENDRR. Meanwhile, SRSF1 directly bound to MST1R, while this phenomenon was partially reversed by FENDRR short interfering RNA. FENDRR could interact with SRSF1 in Kato III cells and the knockdown of FENDRR also induced the apoptosis of GC cells. In conclusion, the findings of the present study suggested that the lncRNA FENDRR may function as an oncogene during the progression of GC by regulating alternative splicing of MST1R and SRSF1 expression levels. lncRNA FENDRR may serve as a potential marker for the diagnosis or target for the treatment of GC. D.A. Spandidos 2021-08 2021-05-25 /pmc/articles/PMC8170639/ /pubmed/34093754 http://dx.doi.org/10.3892/etm.2021.10230 Text en Copyright: © Zhou et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Zhou, Donghui
Zhu, Xiaohua
Wu, Xuan
Zheng, Jingjing
Tou, Laizhen
Zhou, Yong
The effect of splicing MST1R in gastric cancer was enhanced by lncRNA FENDRR
title The effect of splicing MST1R in gastric cancer was enhanced by lncRNA FENDRR
title_full The effect of splicing MST1R in gastric cancer was enhanced by lncRNA FENDRR
title_fullStr The effect of splicing MST1R in gastric cancer was enhanced by lncRNA FENDRR
title_full_unstemmed The effect of splicing MST1R in gastric cancer was enhanced by lncRNA FENDRR
title_short The effect of splicing MST1R in gastric cancer was enhanced by lncRNA FENDRR
title_sort effect of splicing mst1r in gastric cancer was enhanced by lncrna fendrr
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170639/
https://www.ncbi.nlm.nih.gov/pubmed/34093754
http://dx.doi.org/10.3892/etm.2021.10230
work_keys_str_mv AT zhoudonghui theeffectofsplicingmst1ringastriccancerwasenhancedbylncrnafendrr
AT zhuxiaohua theeffectofsplicingmst1ringastriccancerwasenhancedbylncrnafendrr
AT wuxuan theeffectofsplicingmst1ringastriccancerwasenhancedbylncrnafendrr
AT zhengjingjing theeffectofsplicingmst1ringastriccancerwasenhancedbylncrnafendrr
AT toulaizhen theeffectofsplicingmst1ringastriccancerwasenhancedbylncrnafendrr
AT zhouyong theeffectofsplicingmst1ringastriccancerwasenhancedbylncrnafendrr
AT zhoudonghui effectofsplicingmst1ringastriccancerwasenhancedbylncrnafendrr
AT zhuxiaohua effectofsplicingmst1ringastriccancerwasenhancedbylncrnafendrr
AT wuxuan effectofsplicingmst1ringastriccancerwasenhancedbylncrnafendrr
AT zhengjingjing effectofsplicingmst1ringastriccancerwasenhancedbylncrnafendrr
AT toulaizhen effectofsplicingmst1ringastriccancerwasenhancedbylncrnafendrr
AT zhouyong effectofsplicingmst1ringastriccancerwasenhancedbylncrnafendrr