Principal component analysis is limited to low-resolution analysis in cryoEM

Principal component analysis (PCA) has been widely proposed to analyze flexibility and heterogeneity in cryo-electron microscopy (cryoEM). In this paper, it is argued that (i) PCA is an excellent technique to describe continuous flexibility at low resolution (but not so much at high resolution) and...

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Detalles Bibliográficos
Autores principales: Sorzano, Carlos Oscar S., Carazo, Jose Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2021
Materias:
Research Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8171071/
https://www.ncbi.nlm.nih.gov/pubmed/34076596
http://dx.doi.org/10.1107/S2059798321002291
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author Sorzano, Carlos Oscar S.
Carazo, Jose Maria
author_facet Sorzano, Carlos Oscar S.
Carazo, Jose Maria
author_sort Sorzano, Carlos Oscar S.
collection PubMed
description Principal component analysis (PCA) has been widely proposed to analyze flexibility and heterogeneity in cryo-electron microscopy (cryoEM). In this paper, it is argued that (i) PCA is an excellent technique to describe continuous flexibility at low resolution (but not so much at high resolution) and (ii) PCA components should be analyzed in a concerted manner (and not independently).
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spelling pubmed-81710712021-06-14 Principal component analysis is limited to low-resolution analysis in cryoEM Sorzano, Carlos Oscar S. Carazo, Jose Maria Acta Crystallogr D Struct Biol Research Papers Principal component analysis (PCA) has been widely proposed to analyze flexibility and heterogeneity in cryo-electron microscopy (cryoEM). In this paper, it is argued that (i) PCA is an excellent technique to describe continuous flexibility at low resolution (but not so much at high resolution) and (ii) PCA components should be analyzed in a concerted manner (and not independently). International Union of Crystallography 2021-05-19 /pmc/articles/PMC8171071/ /pubmed/34076596 http://dx.doi.org/10.1107/S2059798321002291 Text en © Sorzano & Carazo 2021 https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Research Papers
Sorzano, Carlos Oscar S.
Carazo, Jose Maria
Principal component analysis is limited to low-resolution analysis in cryoEM
title Principal component analysis is limited to low-resolution analysis in cryoEM
title_full Principal component analysis is limited to low-resolution analysis in cryoEM
title_fullStr Principal component analysis is limited to low-resolution analysis in cryoEM
title_full_unstemmed Principal component analysis is limited to low-resolution analysis in cryoEM
title_short Principal component analysis is limited to low-resolution analysis in cryoEM
title_sort principal component analysis is limited to low-resolution analysis in cryoem
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8171071/
https://www.ncbi.nlm.nih.gov/pubmed/34076596
http://dx.doi.org/10.1107/S2059798321002291
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